The student-centered “new three centers” education concept is the direction of teaching reform in colleges and universities. Medical laboratory animal science is an important foundation subject of medical education. To fully motivate student subjective initiative, develop their experimental skills and scientific research thinking, the teaching model of medical laboratory animal science gradually changed from traditional concept of teaching knowledge to centered on students, such as a flipped classroom, online and offline mixed teaching, MOOC, and other new modes. This article examines the knowledge framework and teaching important difficult points on the basis of the curriculum features, explores the advent of the era of the new three centers education concept on the basis of curriculum features, achieves improving teaching quality in course teaching and the scientific literacy in medical students by teaching method innovations and an intelligent teaching environment. “New three center” education promotes a combination of college ideological and political education with professional theory courses for a good synergistic effect with the teaching content of medical laboratory animal science.