Objective To observe changes in blood glucose, bone strength, and intestinal microflora in db/ db mice. Methods Six male db/ db mice and six wild-type(WT)mice at 20 weeks of age were used to assess fasting blood glucose. A three-point bending test was performed to assess mechanical parameters of the femur. 16S rRNA high-throughput sequencing technology was used to sequence the V3-V4 gene region of intestinal contents and analyze the bioinformation. Results The body weight(P< 0. 01) and blood glucose(P< 0. 05) of mice in the db/ db group were increased significantly. Tibial stiffness, maximum displacement, maximum energy absorption, and maximum load of db/ db mice were significantly lower than those of WT mice(P<0. 01). The Chao1 index, ACE index, and observed species in the α-diversity index of db/ db mice were decreased significantly(P<0. 05). There were significant differences in the intestinal bacterial community composition between the two groups(ANOSIM: P=0. 02). Lachnospira was significantly decreased in db/ db mice(P<0. 01). Desulfovibrionales, Proteobacteria, and Deltaproteobacteria were significantly increased(P<0. 05), and Alloprevotella was increased significantly(P<0. 01). Conclusions db/ db mice with hyperglycemia and decreased bone strength are suitable animal models of T2DOP. The diversity of intestinal flora and the abundance of specific flora were different between db/ db and WT mice, which provides a theoretical basis to study intestinal flora and diabetic osteoporosis.