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Home > Archive>Volume 33, Issue 8, 2023 >114-121. DOI:10. 3969 / j.issn.1671-7856. 2023. 08. 015
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Treatment of moderate traumatic brain injury in mice by coadministration of nerve growth factor and penetratin
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1. School of Basic Medicine, Anhui Medical University, Hefei 230032, China.2. Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850

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R-33

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    Abstract:

    Objective To evaluate the therapeutic potential of nerve growth factor (NGF) with penetratin on moderate traumatic brain injury (TBI). Methods The effect of various cell-penetrating peptides combined with NGF on membrane penetration was analyzed by fluorescence immunocytochemistry in cells in vitro, and the optimal molar ratio was selected. Subsequently, rotarod performance and Morris water maze tests were performed to analyze behavioral changes of the moderate TBI mouse model in each group after administration, including coordinative motor ability and the spatial memory function. GFAP and NeuN protein expression was observed by immunohistochemistry. Results Fluorescence immunocytochemistry showed that the content of NGF protein in cells of the NGF/ penetratin group was significantly higher than that in the other groups, and the fluorescence intensity was the highest at a molar ratio of 20 ∶ 1 (penetratin:NGF). Behavioral test showed that the coordination motor function and spatial memory ability of mice in the NGF/ penetratin group were significantly better than those in TBI and NGF groups. Additionally, NeuN protein expression in brain tissue of mice in the NGF/ penetratin group were also significantly higher than that in TBI and NGF groups, while GFAP protein expression in brain tissue was significantly lower than that in the two groups (P<0. 05). Conclusions Penetratin as a drug delivery carrier promotes NGF to cross the blood-brain barrier and enter damaged brain tissue, which has a good therapeutic effect on moderate TBI mice.

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History
  • Received:February 21,2023
  • Online: November 09,2023
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