+Advanced Search
Home > Archive>Volume 33, Issue 8, 2023 >28-37. DOI:10. 3969 / j.issn.1671-7856. 2023. 08. 005
PDF HTML XML Export Cite reminder
Effects of various ischemia times on myocardial ischemia-reperfusion injury in the Chinese miniature swine model
CSTR:
[cstr]
Author:
Affiliation:

1. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Chinese Materia Pharmacology, National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases, Beijing 100091, China. 2. Heilongjiang University of Chinese Medicine, Harbin 150040

Clc Number:

R-33

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Objective To investigate various ischemia times of myocardial ischemia-reperfusion injury (MI/ RI) in Chinese miniature swine and provide the basis to establish the optimal model of MI/ RI in Chinese miniature swine. Methods Twenty-nine Chinese miniature swine were randomly divided into a sham group (n=6), 30 min ischemia group (n=7), 60 min ischemia group (n=7), and 90 min ischemia group (n=9). After pentobarbital sodium anesthesia, left coronary angiography was performed in each model group, and a balloon was exchanged into the middle of the left anterior descending branch through a wire. We elevated the ST segment on a body surface electrocardiogram and the numbers of ventricular fibrillations and defibrillations during the operation. Left ventricular ejection fraction (EF) and left ventricular fractional shortening (FS) were recorded before the operation, before reperfusion, and at 1, 2 and 3 h of reperfusion. Serum levels of creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured at the same time. The area of myocardial infarction was observed by double staining with TTC/ Evans blue. The non-reflowed area after reperfusion was observed by thioflavin S staining. Results Mortality was the highest in the 90 min ischemia group. The numbers of ventricular fibrillations and defibrillations were increased significantly with increasing ischemia time (P<0. 05, P<0. 01). Ejection fraction and fractional shortening were obviously lower after 90 min of ischemia and ay 3 h of reperfusion compared with those in the other groups (P<0. 05, P<0. 01). No significant differences were found in the electrocardiogram, areas of myocardial infarction and non-reflow, myocardial tissue staining, or serum levels of CK-MB, LDH, SOD, and MDA among model groups. Conclusions A swine model of MI/ RI with 90 min of ischemia had strong arrhythmias and high mortality, whereas models established under ischemia for 30 min with reperfusion for 3 h replicated the characteristic lesions of clinical disease with low mortality and are suitable for studies of pathogenesis and drug actions.

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:November 12,2022
  • Online: November 09,2023
Article QR Code
You are the first Visitors
® 2025 All Rights Reserved
Address：5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China Postcode： Phone：86-10-67779337 E-mail:bjb@cnilas.org
Supported by:Beijing E-Tiller Technology Development Co., Ltd.