Effects of low-dose BPA and DEHP on AKR1C3 expression in the adult rat prostate

doi:10. 3969 / j.issn.1671-7856. 2023. 10. 006

Home > Archive>Volume 33, Issue 10, 2023 >38-44. DOI:10. 3969 / j.issn.1671-7856. 2023. 10. 006

Effects of low-dose BPA and DEHP on AKR1C3 expression in the adult rat prostate
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                        10. 3969 / j.issn.1671-7856. 2023. 10. 006
                    
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                        HUANG DongyanHUANG Dongyan
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai 200032. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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WU ShuangshuangWU Shuangshuang
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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SHAO CongcongSHAO Congcong
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai 200032. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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SU XinSU Xin
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai 200032. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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YANG RongfuYANG Rongfu
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai 200032. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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WANG KaiyueWANG Kaiyue
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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ZHOU PingZHOU Ping
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai 200032. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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WU JianhuiWU Jianhui
1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai 200032. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
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Affiliation:1.Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, China. 2. NHC Key Laboratory of Reproduction Regulation, Shanghai 200032. 3. Reproductive and Developmental Research Institute of Fudan University, Shanghai 200032
Clc Number:R-33
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Abstract:

Objective To investigate the effects of low doses of bisphenol A (BPA) and di (2-ethyl) hexyl phthalate (DEHP) on aldo-keto reductase 1C3 (AKR1C3) expression in adult SD rats. Methods Fifty-six adult male SD rats were randomly divided into seven groups (n =8 rats in each group) and administrated BPA (10, 30 and 90 μg/ kg, i. g. ), DEHP (30, 90 and 270 μg/ kg, i. g. ), or the vehicle once a day for 4 weeks. The animals were sacrificed on the day after the last treatment. Blood was collected, and prostate tissues were dissected and categorized into different lobes. Levels of AKR1C3 in serum and prostate were measured by an enzyme-linked immunosorbent assay, and AKR1C3 expression in each prostate lobe was analyzed by immunohistochemistry. Results After BPA administration, AKR1C3 expression in the ventral prostate was increased, and a significant difference was found in the 90 μg/ kg group (P<0. 05). AKR1C3 protein expression in the dorsal prostate was increased, and a significant difference was found in the 10 μg/ kg group (P<0. 01, P< 0. 001). After DEHP administration, the serum level of AKR1C3 in the 270 μg/ kg group was significantly higher than that in the control group (P<0. 001), the AKR1C3 level in the ventral prostate was significantly higher than that in the control group (P<0. 05, P<0. 01), AKR1C3 protein expression was increased, and a significant difference was found in the 270 μg/ kg group (P<0. 05). The AKR1C3 level in the dorsal prostate of the 30 and 90 μg/ kg groups was higher than that in the control group (P<0. 001, P<0. 05). Conclusions Low-dose BPA and DEHP promotes AKR1C3 expression in the prostate of adult SD rats, but the sensitivities of ventral and dorsal prostate lobes to BPA and DEHP are different.

Key words:environmental endocrine disruptor; bisphenol A; Di (2-ethylhexyl) phthalate; aldo-keto reductase 1C3; estrogen; androgen

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Received:February 25,2022
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Online: December 29,2023
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