Objective To investigate the modeling process and model establishment rate of the N-methyl-Nnitrosourea(MNU)-induced lower jiao dampness-heat syndrome uremia bladder cancer (BC) model. Methods Batch samples were collected by bladder perfusion (MNU). HE staining and pathological observation of pathological tissues were carried out at six time points before, during, and after modeling to understand the formation and development of bladder carcinoma in situ. Results The experimental process was smooth, and no obvious urinary tract bleeding in rats occurred during the operation. Using this modeling technique, considerable bladder tumors were formed after week 8, and obvious epithelial hyperplasia, damage and large-scale tumor formation were observed in the bladder of rats in the model group. Conclusions MNU induces an SD rat bladder cancer model with obvious model establishment and low mortality. The experimental data provide a reference to establish, improve and apply the bladder cancer model.