HE Yang
1. School of Nursing, Dalian Medical University, Dalian 116044, China. 2. Capital Medical University School of Basic Medical Sciences, Beijing 100069ZHANG Xiulin
2. Capital Medical University School of Basic Medical Sciences, Beijing 100069. 3. Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing 100037ZHANG Qiuyu
2. Capital Medical University School of Basic Medical Sciences, Beijing 100069ZHANG Xiaolu
2. Capital Medical University School of Basic Medical Sciences, Beijing 100069FU Bo
2. Capital Medical University School of Basic Medical Sciences, Beijing 100069WANG Wendong
2. Capital Medical University School of Basic Medical Sciences, Beijing 100069DU Xiaoyan
2. Capital Medical University School of Basic Medical Sciences, Beijing 100069CHEN Zhenwen
2. Capital Medical University School of Basic Medical Sciences, Beijing 100069LI Changlong
2. Capital Medical University School of Basic Medical Sciences, Beijing 1000691. School of Nursing, Dalian Medical University, Dalian 116044, China. 2. Capital Medical University School of Basic Medical Sciences, Beijing 100069. 3. Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing 100037
R-33
Objective To establish a genetic monitoring method for laboratory quails. Methods Quail microsatellite loci were searched in the literature, and microsatellite DNA loci suitable for quails were screened by an interspecific transfer method in closely related species, namely chickens and ducks. Quail liver DNA was extracted as a template, and the corresponding loci were screened by PCR amplification and agarose gel electrophoresis. On the basis of amplification of the selected microsatellite loci, the number of alleles, polymorphisms, and microsatellite loci combinations for quail genetic quality detection were selected and detection method were developed. Results We preliminary determined 23 microsatellite loci for genetic monitoring of closed-colony laboratory quails. Conclusions A genetic monitoring method for laboratory quails was preliminary developed.
