Analysis of immune cells and their subsets in peripheral blood and spleen of CAST/ EiJ mice

doi:10.3969/j.issn.1671-7856.2024.04.001

Home > Archive>Volume 34, Issue 4, 2024 >1-10. DOI:10.3969/j.issn.1671-7856.2024.04.001

Analysis of immune cells and their subsets in peripheral blood and spleen of CAST/ EiJ mice
DOI:
                        10.3969/j.issn.1671-7856.2024.04.001
                    
CSTR:
                        [cstr]
                    
Author:
                        LIU QiLIU Qi
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
HOU YongzhiHOU Yongzhi
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI NaLI Na
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHANG JingjingZHANG Jingjing
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LU JiahanLU Jiahan
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
CONG ZheCONG Zhe
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHU LinZHU Lin
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
XUE JingXUE Jing
Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing 100021, China
Clc Number:R-33
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Objective To explore the possible causes of CAST/ EiJ mouse susceptibility to multiple pathogens,the immune cell phenotypes in the peripheral blood and spleen of CAST/ EiJ mice were analyzed to clarify their composition. Methods Classical dendritic cells (cDCs), natural killer (NK) cells, B lymphocytes, T lymphocytes, and their subsets in the peripheral blood and spleen of CAST/ EiJ mice and C57BL/ 6J mice were detected by flow cytometry using the cell surface markers CD3, CD4, CD8, CD11b, CD11c, CD19, CD27, CD49b, and TCRβ. Results There was no significant difference in the proportion of cDCs between CAST/ EiJ and C57BL/ 6J mice, but the cDC1 cell subset population was smaller in CAST/ EiJ. The proportions of NK cells ( mainly mature NK cell subsets) and T lymphocytes(mainly CD8⁸⁺T cells) were both lower in CAST/ EiJ mice than C57BL/ 6J mice, while the proportion of B cells was higher in CAST/ EiJ mice than C57BL/ 6J mice. Conclusions The proportions of NK and T lymphocytes in CAST/ EiJ mice were lower than those in C57BL/ 6J mice.

Key words:CAST/ EiJ mice; C57BL/ 6J mice; classical dendritic cells; natural killer cells; B lymphocytes;T lymphocytes

Get Citation

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:January 05,2024
Revised:
Adopted:
Online: June 20,2024
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Share

Article Metrics

History

Article QR Code