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Home > Archive>Volume 34, Issue 4, 2024 >73-83. DOI:10.3969/j.issn.1671-7856.2024.04.009
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Hypoglycemic effect of Chinese yam polysaccharide on DEX-induced IR-3T3-L1 adipocytes
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1. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Risk Assessment Laboratory of Agricultural Products Processing Quality and Safety, Key Laboratory of Agricultural Products Quality and Safety Collection, Storage and Transportation Control, Ministry of Agriculture and Rural Affairs, Beijing 100193, China. 2. Sino-Portugal TCM International Cooperation Center, the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000. 3. Sino-PT Research Center for Medicinal and Food Plant Resources, Department of Biology, University of Minho, Braga 4710-057,Portugal

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R-33

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    Abstract:

    Objective To examine the hypoglycemic effect of Chinese yam polysaccharide on the 3T3-L1 insulin resistance cell model. Methods IR-3T3-L1 adipocytes were randomly divided into control group ( Con), model group (DEX), metformin group (Met, positive drug group) and CYPS group (0. 05, 0. 15, 0. 45 mg / mL). After the creation of the IR-3T3-L1 cell model by dexamethasone (DEX) (1 μmol / L) stimulation, Chinese yam polysaccharide treatment was applied. Glucose intake and the levels of TC, TG, LDL-C, HDL-C, GSH-Px, MDA, HK, and PK were then measured.AMPK-PI3K/ Akt signaling pathway-associated gene expression was identified using qRT-PCR. Results The glucose consumption of IR-3T3-L1 cells was considerably decreased after 48 hours of treatment with 1 μmol / L DEX compared to that of the Con group (P<0. 01), and the reduction persisted for 60 hours. 0. 05, 0. 15, 0. 45 mg / mL CYPS treatment significantly increased glucose consumption (P<0. 01), HK, PK, GSH-Px enzyme activities (P<0. 01), HDL-C content (P<0. 01), and decreased MDA enzyme activity (P<0. 01), T-CHO, TG, LDL-C content (P<0. 01) in IR-3T3-L1 cells. 01). Additionally, CYPS administration dramatically decreased PI3K, Akt, GLUT-4, AMPK, IRS-2, PPARa, and adiponectin mRNA expression levels (P<0. 05) and reduced IRS-1, GSK-3β, ACC, and FAS mRNA expression levels (P<0. 01). Conclusions In IR-3T3-L1 cells, CYPS can reduce oxidative stress, control lipid metabolism disorders, and enhance DEX-induced glucose intake. AMPK-PI3K/ Akt signaling pathway-associated genes may be connected to the process.

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History
  • Received:January 11,2024
  • Online: June 20,2024
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