An animal biosafety level-3 laboratory (ABSL-3) is a high-level biosafety installation that can conduct experiments on animals infected with highly pathogenic microorganisms. In recent years, with the continuous characterization of emerging and re-emerging infectious diseases, high-level biosafety laboratories have played increasingly important roles in pathogenic mechanism and drug and vaccine research and development. The demand for ABSL-3 is increasing year by year. At the same time, there is also a growing demand for personnel who are competent in working in ABSL-3. The systematization, normalization, and standardization of pre-service training have become important to guarantee a reduction in the risks to personnel working in ABSL-3. Training of ABSL-3 staff needs to be carried out in specific simulated laboratories. Therefore, it is necessary to construct simulated ABSL-3 and establish scientific and effective operating standards and mechanisms. This paper comprehensively introduces the design, construction, operation,and functions of a simulated ABSL-3 installation