Impact of hypoxia / reoxygenation on endoplasmic reticulum stress in hippocampal cells
CSTR:
Author:
Affiliation:

1. Graduate School of Qinghai University,Xining 810016, China. 2. Graduate Management Department of Affiliated Hospital of Qinghai University, Xining 810001. 3. Plateau Medicine Research Center,Medical College of Qinghai University, Xining 810001. 4. Key Laboratory of Qinghai Applied Basic Sciences, Xining 810001

Clc Number:

R338. 2

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To investigate the effects of hypoxia / reoxygenation (H/ R) on hippocampal neurons and endoplasmic reticulum (ER) stress in mice. Methods HT22 hippocampal neurons were used for subsequent experiments after H/ R treatment. The effects of H/ R on the proliferation and migration of HT22 hippocampal neurons were detected by Cell Counting Kit-8 and scratch assays and its effects on the morphology of the ER was observed by transmission electron microscopy. The effect of H/ R on the development of HT22 hippocampal neurons was observed by immunofluorescence, and its effects on ER stress in hippocampal neurons were detected by Western blot and quantitative reverse transcription-polymerase chain reaction. Results Compared with the Con group, H/ R inhibited the proliferation and migration of HT22 hippocampal neurons and decreased the expression of the neurodevelopmental protein brain-derived neurotropic factor(P<0. 05). H/ R also reduced the expression of the synaptic plasticity proteins postsynaptic density protein-95 and α-synuclein(P<0. 05). H/ R provoked pronounced ER swelling and up-regulated the key ER-stress markers glucose-regulated protein 94, binding immunoglobulin protein, and activating transcription factor 6, thereby intensifying ER stress(P<0. 05). Conclusions H/ R has a damaging effect on HT22 hippocampal neurons, which may be related to enhanced ER stress.

    Reference
    Related
    Cited by
Get Citation
Related Videos

Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:May 15,2025
  • Revised:
  • Adopted:
  • Online: May 06,2025
  • Published:
Article QR Code