Animal biosafety laboratories generally use negative-pressure cages to raise animals, to guarantee biosafety. Laboratory monbeys are much larger than the rodents, so they are mostly housed in non-airtight negative pressure animal isolation equipments within animal biosafety laboratories. Regarding practical work experience in an Animal Biosafety Level 2 (ABSL-2) laboratory, this study discusses how to control biological risks at various stages of monkey breeding and infectious experiments within an ABSL-2 laboratory. This exploration provides valuable insights and references for controlling biological risks in ABSL-2 and ABSL-3 laboratories carrying out infection experiments in monkeys in the future.