Recent rapid developments in molecular biological techniques have allowed the use of gene editing, as a means of genome modification, for the establishment of experimental animal models, with high efficiency, accuracy, and flexibility. This article mainly summarizes the construction and application of the latest gene-editing techniques in animal models, including pigs, non-human primates and dogs. It provides a theoretical reference for the application and in-depth study of gene-editing techniques in large experimental animals, which may better simulate human diseases, and for further studies of the potential pathogenesis of biomedical and human complex diseases.