Bacterial reinfection in the lungs of patients with influenza virus (IV) is a key factor leading to serious illness and death. The adverse consequences caused by co-infection with IV and Streptococcus pneumoniae (SPN) impose a serious burden on patients. However, the specific pathogenesis is complex, how to make better use of the mouse animal model of IV/ SPN co-infection for subsequent basic research is of great significance. This article reviews the selection of animals, the selection of pathogen types, the selection of different modeling times, the identification of the co-infection model, and its applications in the IV/ SPN co-infection model, so as to provide a reference for the selection of animal models of IV/ SPN co-infection in the future.