We aimed to establish a scientific regulatory system to improve the supervision of laboratory animal welfare and ethics. The system included 12 regulatory indexes, and targeted evaluation criteria were formulated based on the characteristics of the indexes. The regulatory indexes were supervised by inspecting the sites and using specialized techniques such as small-animal micro-computed tomography and magnetic resonance imaging. The system established a dynamic regulatory mechanism combining project approval review, process supervision, and final review. The system also implemented a regulatory process including both online and offline method. This system will help to prevent the occurrence of incidents that violate technological ethics, standardize regulatory procedures, and provide a reference for the supervision of welfare and ethics in the production and use of laboratory animals.