Laboratory animal facilities play an important role in scientific research in hospitals. Their construction standards and specifications, design principles, functional layout, equipment configuration, and environmental control, as well as their operational management, quality control, safety management, personnel training, and other aspects should all follow unified standards and requirements. The complex and systematic nature of the construction of laboratory animal facilities, however, mean that the design concept, purpose, geographical location, construction requirements, personnel composition, and other aspects of the facilities differ among research institutions and hospitals. Different problems are also encountered in the specific construction and operation practice of laboratory animal facilities. Here, we discuss the construction and operation of laboratory animal facilities at the Central China Subcenter of the National for Cardiovascular Disease. We summarize the experience of our unit in the construction and operation of laboratory animal facilities, to provide theoretical guidance and a practical reference for the construction and operation of laboratory animal facilities in the hospital system.