Research progress in primary culture and identification of penile cavernosum cells
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1. Department of Urology, the Affiliated Wenling Hospital of Wenzhou Medical University, Wenling 317500, China.2. the First School of Clinical Medicine, Zhejiang University of Chinese Medicine, Hangzhou 310053. 3. Department of Urology, Affiliated Hospital of Nantong University, Nantong 226001. 4. National Flagship Construction Department of Collaboration Between Traditional Chinese Medicine and Western Medicine (Urology Department), Nantong 226001

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R-33

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    Abstract:

    The primary isolation and culture of penile corpus cavernosum cells are key techniques for studying the pathological mechanisms of erectile dysfunction (ED). This review summarizes the isolation and culture method of the major cell types in the penile corpus cavernosum, including smooth muscle cells, endothelial cells, pericytes, and fibroblasts. We discuss commonly used techniques, including enzymatic digestion, tissue explantation, immunomagnetic bead separation, and Matrigel induction, and compare their advantages and disadvantages. We also summarize the method for identifying different cell types. This systematic review of cell culture and identification techniques thus provides technical support and references for further research into the roles of penile corpus cavernosum cells in the onset, progression, and treatment of ED.

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History
  • Received:January 16,2025
  • Revised:
  • Adopted:
  • Online: January 29,2026
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