Objective To assess the effects of long-term feeding of transgenic cry1Ab / cry2Aj and G10evoepsps insect-resistant and herbicide-tolerant maize on bone mineral content ( BMC) and density ( BMD) in two generations of Macaca fascicularis. Methods A total of 24 parental ( F0) cynomolgus monkeys were divided into 70% genetically modified corn(GM; n= 8), 70% non-genetically modified corn (Non-GM; n= 7), and normal feed control groups (Normal; n= 9). The offspring (F1) generation included 46 Macaca fascicularis, comprising 16 in the GM group, 13 in the Non-GM group, and 17 in the Normal group, with age ranges spanning 1 ~ 6 years. Lunar densitometers were used to scan and analyze BMC and BMD in the head, arms, legs, pelvis, spine, and whole body of animals in each group. Results No significant BMC and BMD differences ( P>0. 05) were observed between groups in the head, arms, legs, pelvis, spine, or whole body at three age stages (1 ~ 2, 3 ~ 4, and 5 ~ 6 years) in both F0 and F1 generations. Conclusions Long-term feeding with insect-resistant and herbicide-tolerant transgenic maize did not significantly affect BMC and BMD in either parental or offspring generations of Macaca fascicularis. This study provides reference data for evaluating the long-term safety of consuming this genetically modified maize.