[Abstract] Objective: To explore the factors in duck hepatitis B virus infection model by different kinds of ducklings and observe the antiviral effect in models. Methods: Collecting duck sera , qualitative detecting viral DNA by PCR method and detecting the changes of viral DNA load by the quantitative PCR method in duck sera. Observing the antiviral effects in duck DHBV infection models after antiviral drug treatment. Results: Natural infection rate of duck DHBV is different, Cherry Valley duck for 8.75%, two batches in Hubei duck were 17.80% and 10.68% respectively. Both intravenous and intraperitoneal injection can cause ducklings infected with DHBV, infection rates of two ways were 80% and 65% respectiviely. Ducks infected with DHBV , the viral load is maintained at 106 ~ 108 copies / mL and persist for more than 20 days. Changing trends of antiviral effect in different DHBV models are coincident after treating by the antiviral drugs. Conclusion: Duck species and artificial routes of infection can affect DHBV infection rate, in vivo the viremia of duck is stably persistent after DHBV infection. Therefore, DHBV infection model is an available model in antiviral drug screening.