Objective To evaluate the influence of Xuebijing (XBJ) by inflammation cytokines of multiple organ dysfunction syndrome (MODS). Methods 90 rats were randomly separated into 3 groups, the control group and XBJ group were infused 15 μg / kg LPS in vena caudalis, Then the physical sign appearance was observed and collection the serum to determine the expression concentration of TNF-α、IL-1α、IL-4 and IL-10 by ELISA in each group at different times. Results ① The death rate of rats were the percent of 0, 37, and 20 in the control group，model group and XBJ group, respectively. ② The XBJ enhance the increased amplitude of TNF-α and deduce the increased amplitude of IL-1α by compared with model group, the expression concentration peak of TNF-α and IL-1α is 6h~24h. ③ The expression concentration of IL-4 in model group and XBJ group in model group has significant ascendant compared with control group at 6h~48h; XBJ group has significant have significant ascendant compared with control group at 6h~12h (p<0.05).The expression concentration of IL-10 ascendant compared with control group at 6h (p<0.05). Conclusion The results demonstrated that XBJ could degrade the death rate of MODS induced by LPS，and it could reduce the increased amplitude of TNF-α, whereas enhance the increased amplitude of the serum IL-1α,IL-4 and IL-10, it could also prevent those rats from changing into MODS efficiently.