Objective To compare the effect of the two vitrification of embryos frozen invarious mouse strains . Methods The two-cell embryos from six mouse strains(KM、ICR、BALB/c、C57BL/6J、OB/OB、LAP/rtTA0F59) were cryopreserved and thawed respectively with EFS and DAP cryoprotectant . The thawed rate in vitro and the implantation rate in vivo were determined to evaluated the two ways. Results The postthaw survival rates of different strains in EFS were average 69.97% ( 47.9%～83.6% ), and DAP were average 47.23%(26.3%～76.7%). After 2-cell embryos were transferred to the oviducts of pseudopregnant recipient in all strains, the implantation rates in EFS were ranged from 1.75% to 45.0% (average 27.23%) and DAP were ranged from 7.0%～46.3% (average 31.43%). With the respect of thawed rates, EFS was preferred to DAP. No significant differences(P＞0.05) were found in implantation rates of EFS and DAP on KM and ICR strains. But the implantation rates of BALB/c、C57BL/6J、OB/OB、LAP/rtTA0F59 in EFS were higher than those in DAP respectively. Conclusion The both of KM and ICR mouse were suitable for EFS cryoprotectant. EFS and DAP cryoprotectant could be used to froze embryos of C57BL/6J、OB/OB、LAP/rtTA0F59 mouse strain, then DAP was superior to EFS. But it is necessary that the further study on embryos cryopreservation in BALB/c mouse in future.