Aim To assess the sustainability of visceral hypersensitivity model induced by rectal distention with limbs constraint. Methods Thirty-two 8-week Sprague-Dawley(SD) rats were randomly divided into control group (n = 16), model group (n = 16), rectal distention with limbs constraint lasting for 2 weeks was operated on model group, visceral sensitivity was evaluated by abdominal withdrawal reflex(AWR). Based on the AWR score, control group were randomly divided into group A (n = 8) and group B (n = 8), model group were randomly divided into group C (n = 8) and group D (n = 8). Colon specimens of group A and group C were obtained Under anesthesia. Group B and group D received normal breeding until 18 weeks old, and then group D received the same stimulation. AWR score and colon specimens were obtained in the same way. ultrastructure of mast cell was observed by electron microscopy, toluidine blue staining was performed to count mast cell(MC), protease activated receptor 2 (PAR2) was analyzed by Western Blot. Results AWR score of group C was higher than that of group A, group D lower than group C, the differences had significance (P < 0.01). Intestinal MC count of group C was higher than that in group A, group D lower than group C, the differences had significance (P < 0.01). Mast cell degranulation was visible in group C, neither other groups. PAR2 expression level of group C was higher than that of group A, group D lower than group C, the differences had significance (P < 0.01). Conclusion Visceral sensitivity of visceral hypersensitivity model induced by rectal distention with limbs constraint decreased along with the increase of weeks.