[Abstract] Objective To analyse and evaluate the genetic quality of Fangshan closed colony miniature pig population in DNA level according to DB11/T828.3-2011. Method According to the local standard DB11/T828.3-2011, 25 pairs of microsatellite primers were used in 22 Fangshan closed colony miniature pigs for polymerase chain reaction. Popgen32 was used for result handler. Results Among 25 pairs of primers, 4 of that were not able to get stable amplification results, even when changing reaction condition; 21 pairs of primers were able to get stable amplification results. There are 91 valid allelotype in 21 microsatellite locus. The average PIC is 0.4630, and the average heterozygosity is 0.5331. What’s more, products of SWr1008 is different from that of DB/T828.3-2011. And the chromosome position of SW271 in DB/T828.3-2011 and http://www.marc.usda.gov also exists differences. Conclusion Fangshan closed colony miniature pigs with genetic stability satisfy the genetic standard of closed colony animal.