RANBP9 targeted the expression of TGF-β1 induced the cell apoptosis in colorectal cancer Colo320 Cell
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The First Affiliated Hospital of Hebei North University

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    Abstract:

    objective: To investigate Ran binding protein in microtubule-organization center (RANBP9) targeted the expression of transforming growth factor β1 (TGF-β1) and its effect on colorectal cancer Colo320 cell apoptosis. Methods: The expression data of RANBP9 (gene)? in 625 cases of colon cancer tissues and 20 cases of normal colon tissues in the TCGA database (TCGA) were analyzed. KMPLOT was used to analyze the relationship between the expression of RANBP9 and the survival time of colon cancer patients. The human protein immunohistochemistry database was used to analyze the expression of TGF-β1 in normal colon tissues and colon cancer tissues. The UALCAN database was used to analyze the relationship between the expression of TGF-β1 and the survival of colon cancer patients.The dual luciferase experiment analyzes the relationship between RANBP9 and TGF-β1.The pcDNA3.1-GFP-RANBP9 and pcDNA3.1-GFP-RANBP9-NC were transfected into the cells in the experimental group and the control group,respectively. And the normal group of cells will be established without transfection operation and routinely cultured.MTT method was used to detect the growth viability of each group of cells, flow cytometry was used to detect the apoptotic rate of each group of cells, JC-1 staining was used to detect the mitochondrial membrane potential of each group of cells, and Western blot was used to detect the expression of RANBP9 and TGF-β1 in each group of cells. Results: The expression of RANBP9 in colon cancer tissue was significantly reduced. Compared with patients with low RANBP9 expression, colon cancer patients with high RANBP9 expression had a higher survival curve, and the expression of TGF-β1 in colon cancer tissue was significantly increased. Compared with patients with high TGF-β1 expression, patients with low TGF-β1 expression had a higher survival curve, and the differences were statistically significant (all P<0.05).In colon cancer, RANBP9 can target the expression of TGF-β1. Compared with the normal group, the growth viability of the experimental group, the mitochondrial membrane potential, and the expression of TGF-β1 were significantly down-regulated, and the apoptosis rate and the expression of RANBP9 were significantly increased. The differences were statistically significant (all P<0.05).Conclusion: RANBP9 can target the expression of TGF-β1, promote the growth of colon cancer cell Colo320 and decrease the mitochondrial membrane potential, and induce its apoptosis.

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History
  • Received:January 16,2024
  • Revised:April 26,2024
  • Adopted:April 30,2024
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