ABSL-3 laboratory is Animal Biosafety Level-3 laboratory where highly pathogenic microorganisms can be done. In recent years, with the continuous emergence of emerging and re-emerging infectious diseases, high-level biosafety laboratories have played an increasingly important role in the hardware support of pathogenic mechanism, drug and vaccine research and development. The demand for ABSL-3 laboratories is increasing year by year. At the same time, there is also an increasing demand for personnel competence of working in ABSL-3 laboratories. The standardization of pre-service training has become an important guarantee to reduce the risk of personnel working in the ABSL-3 laboratory. The systematized, normalized and standardized training work needs to be carried out in a specific place. Simulated laboratory is the best place. Therefore, it is necessary to establish animal biosafety level-3 simulated laboratory and establish scientific and effective operating standards and mechanisms. This paper introduces the design, construction, operation and function of ABSL-3 simulated laboratory.