This paper introduces the daily management service of experimental animal license in Hubei Province and the supervision matters during and after the event. It expounds the current situation of experimental animal license management, achievements, problems and countermeasures in Hubei Province.The focus was on analyzing the legislative situation of experimental animals in Hubei Province, the issuance and distribution of permits, the scale of facilities, and the composition of employees. In recent years, the number of experimental animal licenses issued in Hubei Province has been increasing year by year, and the number of animal production and use by licensed units has continued to rise. While the related industries are flourishing, there are also some regulatory deficiencies. this paper from the perspective of biosafety, combined with the problems encountered in the license management service work, put forward the relevant safety supervision countermeasures and suggestions, so as to better promote the development of experimental animal industry in Hubei Province.