Objective: We observed the effects of different non-surgical embryo transfer devices, number of transfered embryos, embryo stage, and embryos obtained from differernt mouse strain on the efficiency of non-surgical embryo transfer in mice, as well as compared the efficiency of surgical and non-surgical embryo transfer, to establish a stable non-surgical embryo transfer technology system. Methods and Results: Two different non-surgical transfer devices were used for embryo transfer, and the pregnancy rates were（75±0）%、（66.67±14.43）%，and the birth rates were (46.11±6.31)%, (18.89±0.96)% respectively. The embryo number for transferring is 10, 15, and 20, the pregnancy rates were (66.67±11.55)%, (80.00±0)%, (66.67±23.09)%, and the birth rates were (29.33±4.16)%, (38.67±4.81)%, (17±3.46)% respectively. Blastocysts and morulae were transferred non-surgically, and the pregnancy rates were (80.00±0)%, (146.67±11.55)% respectively, with the birth rates (38.67±4.81)%, (10.22±2.77)%. Four strains (C57, ICR, genetically modified mouse A, genetically modified mouse B) were used as donors for non-surgical embryo transfer, and the pregnancy rates were (66.67±11.55)%, (80.00±0)%, (73.33±11.55)%, (80.00±0)%, with the birth rates (26.67±2.67)%, (38.67±4.81)%, (32±3.53)%, (29.34±2.31)%respectively. 15 pseudo-pregnant mice were transplanted surgically, and 15 pseudo-pregnant mice were transplanted non-surgically, and the pregnancy rates were (80.00±0)%, (86.67±11.55)% respectively. The birth rates were(38.67±4.81)%, (36±5.82)% respectively. Conclusions: The efficiency of non-surgical embryo transfer using the A transfer device was higher on our platform. The embryo transfer efficiency was higher when 15 embryos were transfered into unilateral uterine horns of pseudo-pregnant 2.5-day recipients. The efficiency of blastocyst-stage embryos transfer was higher than morula. There was no significant difference in the efficiency of surgical embryo transfer and non-surgical embryo transfer.