Animal biosafety laboratories generally use negative-pressure cages to raise animals to guarantee biosafety. The monkeys are much larger when compared with the rodents. The monkeys are mostly housed in negative-pressure rearing cages within animal biosafety laboratories in a semi-open form. With practical work experience in an animal Biosafety Level 2 (ABSL-2) laboratory, the author discussed how to control biological risk at various stages of monkey breeding and infectious experiments within the ABSL-2 laboratory. This exploration provides valuable insights and references for controlling biological risks with monkey infectious experiments in ABSL-2 and ABSL-3 laboratories in the future.