Traditional laboratory animal breeding is costly and time-consuming, which causes certain limitations in the teaching practice of Experimental Zoology. Zebrafish, with its unique biological characteristics, has gradually become a widely used experimental animal model. However, there are still relatively few teaching examples related to zebrafish in Experimental Zoology courses. This paper explores the application of zebrafish by incorporating them into two modules-“Tissue Sectioning Techniques” and “Immunological Techniques”-in the course of "Medical Laboratory Animal Science and Technology." The aim is to utilize their advantages in experimental zoology teaching and explore their potential applications. This study provides a detailed introduction to the experimental procedures, experimental design, and teaching content involving zebrafish, aiming to offer undergraduate students a more efficient, cost-effective, and practically meaningful experimental teaching platform.