Abstract:对野生长爪沙鼠进行系列实验动物化研究;用乙醚浅麻醉法解决了交配时的殴斗伤亡问题;肯定了一雄一雌频密繁殖法的优点:测定了心、肝、肾、脾等主要脏器重量,发现野生的略大于驯化的;光、电镜检查发现,肾近端小管曲部的细胞基底有明显的基底纹;还测定了26项血清生化值及13项血液细胞的数据;其T淋巴细胞平均值为63.3±2.0;心电图V导联最典型,S-T段缺如,其电激动过程与人相似。

Abstract:A ectromelia-like disease in th colony of wistar strain rats was studied. The clinic signs,dermal lesions of the disease rats are similar to mouse pox. The morbidity of this disease is 44.5%. The fatality^ rate is 11.3%. Type A inclusion bodies were observed in dermal dermis and hypodermis tissues of disease rats as well as in the epithelial cells of the chick embryo chori-oallantoic membrane ( C.E. C.M ) with the pox pock induced by inoculation of the tissue suspention from a disease rat. The disease was reproduced artificially in mice and rats which are good health following inoculation with livers,spleens and dermal necrosis tissue suspension of the disease rats.And the virus was isolated and a cytopathogenic effect was observed in IBRS2 cell line. The disease was also reproduced by the culture suspention of IBRS2 cells with the virus or the suspention of homogenized tissue of C.E. C.M which was inoculated with the suspention of the tissues of the disease rats. It is demonstrated by means of the agglutination test coordinated by SPA that the antigenicity of the virue isolated is the same as that of the pathogenic agent. Under the electron microscope viral particles with envelope were morphologically similar to poxvirus and 250-350 nm in diameter.

Abstract:A new medium combined with the use of oblique illamination technique was developed to rapidly identify the colonies of listeria. Results were compared with those reported by foreign authors using media containing antibacterial agents,which showed methods developed in this paper had some advantages in term of rapidness and correctness for identifying listeria. Several selective-enrichment media were comparted and investigated. Results showed that CRT was the best one. Using CRT with new differentiation medium, Sen-etivity of isolating listeria increased 5-10 fold. Method of liver homogenate to isolate listeria from animals was simpified and showed very practical when used with CRT to isolate listeria from artificial infected animals. Methods of CRT and liver homogenate were used to isolate listeria from labora tory animals from some institutes in Beijing. Results were negative.

Abstract:A survey of parasites on medical laboratory animals was carried out from 1983 to 1989 in medical laboratory animal center of Tongji medical University. The results were as follws: ( 1 ) 1006 among the 1368 animals ( 73.54%) were naturally parasitized with parasites. (2)17 species protozoa, 16 species of trematodes, 8 species of cestoides, 11 species of nematodes and 6 species of insects were obtained from 1368 animals of 10 species. ( 3 ) and the zoono-tic parasites woth the invasion rate of 36.20% were detected.

Abstract:利用随机引物法反转录合成SRV-1 ss-cDNA和ds—cDNA.将ds—cDNA平端连接到质粒pUC19中,转化E.Coli.DH5α菌.氨苄青霉素、X-gal培基和菌落杂交筛选,克隆阳性重组率为20.7%.EcoRⅠ、BamHⅠ双酶切分析,12.7％的插入片段大于500bp。Southern杂交进一步确证,所选三株重组质粒(pSRV-127、pSRV-156和pSRV-169)的探针用插入片段(0.9kb、1.0kb和1.4kb),是SRV-1 cDNA片段.光敏生物素标记纯化pSRV-169DNA和pSRV-156cDNA片段制备探针,前者用于检测SRV-1病毒RNA,后者用于检测纯化病毒颗粒。碱性磷酸酶系统染色,灵敏度分别为18pg和4×10~5vp

Abstract:Method for chromosome prepration of bone marrow cells and short term cultured lymphocytes from Guinea Pig was slightly improved, in order to prepare good chromosomal samples.The analyses of 218 mitotic plates showed that the diploid chromosome number was 64.20 mitosis were used for measurement of each chromosome, other 20 metaphase cells were used for G-banding, while the banding patterns of each chromosome were also analysed and idiogramed. Consequently polymorphism of No.23 chromosome in mitosis were analysed. The results were briefly discussed.

Abstract:Three species of the genus Syphacia females, from mice, rats and the golden hamster, were described with scanning electron microscopy. There are six papillaes on the cephalic extremities of the S.obvelata and S. mesocriceti. Only four papillaes are seen on the S.muris, having many micro-pore instead of other two papillaes and on the surface of the body of S.muris.No lateral alae prominent is the different beyween S.muris and other two species.S.obvelata is characterized by a lip-like vulva.