• Issue 4,1998 Table of Contents
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    • Discussion on the Education and Training of Laboratory Animal Personnel in China

      1998(4).

      Abstract (1458) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:In recent decades, great development has been achieved in the education and training of laboratory animal personnel in China. The current situation of national education and training of laboratory animal personnel is reviewed in this paper, and the future efforts presented below include:further perfecting the multi -level education and training systems, implementing uniform andstandardized program for .Professional education and training; improving the teaching and researchconditions, stabilizing professional members; and strengthening the ability of commanding information, promoting international and internal exchange of laboratory animal science.

    • >论著
    • Effect of Different Light Period on Growth and Development and Reproductive Performance of KM Mouse in Semi-barrier System

      1998(4):193-196.

      Abstract (1562) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:将清洁级KM鼠分为3组,分别给予短、中、长三种不同的光照处理,10W后称取其体重,并测定睾丸、副睾、卵巢、子宫及肾上腺的重量,同时记录各组母鼠连续三胎的繁殖性能。统计结果表明:无论雄、雌鼠长光照组体重明显高于短光照组,差异极显著(P<0.01);长光照组和中光照组睾丸重量明显高于短光照组.差异极显著(P<0.01);长光照组和中光照组副睾重量显著地高于短光照组(P<0.05);雄鼠肾上腺重量长光照组显著地高于短光照组(P<0.05);卵巢重量表现为长光照组极显著地高于其它两组(P<0.01);子宫重量长光照组显著高于其它两组(P<0.05);雌鼠肾上腺重量仅表现为长光照组显著高于短光照组(P<0.05);母鼠连续三胎繁殖性能显示,短光照组无论是产仔数,仔鼠初生重及仔鼠离乳数均显著地低于长光照组(P<0.05);而仔鼠离乳重及离乳率差异不显著(P>0.05).

    • Aetiological Diagnosis of Lophura nycthemera Tuberculosis

      1998(4):197-202.

      Abstract (1886) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:Lophura nycthemera tuberculosis was first diagnosed by pathogen isolation, biochemistry identification and pathogenicity test. We also studied the tuberculosis diagnosis techniques, prevention and cure of captived exotic birds.

    • Histopathological Observation of Myocardiosis and Nephropathy of Experamental Diabetic Rats

      1998(4):203-206.

      Abstract (1363) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:观察36只链脲佐菌素性糖尿病Wistar大鼠模型.进行心脏、肾脏和胰腺的组织病理学研究,主要改变为:心肌细胞肥大、肌溶解、PAS阳性糖蛋白类物质沉着,间质结缔组织增生,中、小血管增厚,中层平滑肌细胞增生;肾小球增大,毛细血管壁增厚、僵硬,肾入、出动脉增厚.肾小管上皮细胞水样变性,PAS阳性糖蛋白粪物质沉着;胰岛分布稀疏、数量减少,胰岛萎缩.成纤维细胞增生。通过对STZ诱发模型心脏、肾脏和胰岛结构观察说明,该动物模型可用于糖尿病发病机制和药物疗效的评价

    • Establishment of Composite Skin Animal Model on Rats

      1998(4):207-211.

      Abstract (1415) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:It's difficult that apply composite skin to clinicat present. One of reasons is uncertain resultabout decreasing or eliminating its antigen, so we continue do animal experiments, and used Wistar rats as donor, SD rats as receptor to do skin grafting, in order to judge that the antigen abouttwo kinds of rats whether is same or not. We observe the survival rate of grafting flap, histological changes, proliferation function of lymphocyte on POstoperation. The results showed that allo flaps grafted were rejected completely, so the MHC 11 antigen is different between two kinds ofrats. Its gene were not contaminated, and can be used as the reliable model of composite skin.

    • Studies on Removing Ammonia Odor in Air by Biopurification Technigue

      1998(4):212-215.

      Abstract (1529) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:Nitrobacteria, nitrosobacteria and antinitrobacteria which were successfully used in wastewater treating project had been separated and artificially cultured in our laboratory, the predominantbacteria were immunoadsorpted on ceramic filler and made into a minitype equipment of removingammonia. When air with ammonia (NH3) passed through the equipment, the ammonia, were beremoved the results indicated that: the concentration of NH3 was 28~111 mg/m3 when the in fluent volume was separately 0. 12 m3/h, 0. 183m/h, and the concentration in effluent decreased to0. 06~0. 32mg/m3, the efficiency of deodorizing was 99. 19 %~99. 89%, in the equipment PHremained 7. 0. It was certified this system had a high and stable efficiency.

    • Study on Monoclonal Antibodies Against Rabbit Haemorrhagic Disease Virus (RHDV)

      1998(4):216-220.

      Abstract (1847) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:Four strains of hybridoma cells secreting speafic antibodies to RHDV were obtained by cellfusion technique. The RHDV used for immugon was purified by potassium Tartrafe-Glyceraldensity gradient Centrifugation. It was confirmed by western blot that the McAbs react with60KD, 55KD, 38KD and 20KD of RHDV protein. Double-McAb sandwich ELISA for the defection of RHDV antigen was established and analysis of sensitivity and specificity on test systemwas also performed in our laboratory.

    • Study on KM Mice Reproductive Ability of NVSI

      1998(4):221-225.

      Abstract (2055) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:Body weight, weight of internal organs, and reproductive ability of conventional KM micewas studied. The results demonstrated that the average product number of mice was 13. 72 at onelitter, the average weight of mice was 1. 89 grams at birth, and the survival rate of sucking micewas 98. 41 %. The KM mice during the first 2 weeks after birth grew rapidly, it increased 0. 665grams a doy. From the second to the sixth week, the male and female mice increased 0. 7 and 0.55 grams respectively. From the seventh to the tenth week, the male and female mice increased0. 38 and 0. 32 grams respectively. The average number of days between litters were 23. 33.

    • Design of Comuter Management System in Laboratory Animal Center

      1998(4):226-229.

      Abstract (1882) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:The article introduce the function and design method of computer management system baseon Filemake Pro database system.

    • Study on Changes of the Main Organa Weight and Length of Spontaneous NIDDM Chinese Hamsters

      1998(4):230-232.

      Abstract (1999) HTML (0) PDF 0.00 Byte (0) Comment (0) Favorites

      Abstract:The weight and length of the main organa of spontaneous NIDDM chinese hamsters were direct measured and compared with mormal control inbred-lines. The results showed that averageabsolute weight and relative weight of heart, liver and kidney of isonymous sPOntaneous NIDDMchinese hamsters were all higher than that of normal inbred-lines (P< 0. 05 or < 0. 01), andlength of digestive tubes were longer than that of control too, especially in intestinum angustums(P<0. 01 ). This suggested that changes on weight of heart, liver and kidney and length of intestinum angustums were maybe relative to NIDDM.

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