Abstract:本试验探讨了重组人白细胞介素－１β（ｒｈＩＬ－１β）对小鼠桑椹胚玻璃化冷冻后体内外发育的影响。结果表明：当ｒｈＩＬ－１β添加浓度为５、５０、５００ＩＵ／ｍｌ时，对解冻后的胚胎经体外培养，发育率（８７％～１００％）与对照组（９４％）相比无显著性差异（Ｐ＞００５）。而浓度提高到１０００ＩＵ／ｍｌ时，胚胎的发育受到抑制。解冻后胚胎在含有５、５０、５００ＩＵ／ｍｌ浓度的ｒｈＩＬ－１β培养液中培养３～４ｈ后移植于假妊娠３ｄ的受体鼠，移植妊娠率（８６％、１００％、８３％）高于对照组（５６％），特别是５０ＩＵ／ｍｌ处理组与对照组有极显著差异（Ｐ＜００１）。而浓度为１０００ＩＵ／ｍｌ处理组的胚胎移植妊娠率（３０％）低于对照组。试验证明，添加浓度为５～５００ＩＵ／ｍｌｒｈＩＬ－１β的培养液，对解冻后胚胎的体外发育率无影响，而且能够显著提高移植妊娠率。

Abstract:本实验探讨用硫堇和台盼兰染料对妊娠４～５ｄ小鼠进行活体染色以显示胚泡着床位点的可能性。研究结果：１台盼兰可用作胚泡着床点的染色反应，具体方法是：尾静脉注射０５％台盼兰０３ｍｌ，１０ｍｉｎ后观察，而硫堇则无效；２着床点数目在两侧子宫角内的分布有显著差别，左右子宫角内分别为６４０±１６４和４８０±２４０个；３着床点的间距为２２５±０８７ｍｍ。

Abstract:We have confirmed the expression of CCK-A and CCK-B receptor mR NA in the lung tissues of SD rats by reverse transcribed polymerasechain reac tion (RT-PCR). The expression of CCK-A and CCK-B receptor mRNA in the lung was i dentified by means of second-amplification and southern blotting. The experiment showed the RT-PCR product of approximately 1.3kb from the CCK-A receptor mRNA a nd approximately 480bp from the CCK-B receptor mRNA. Our result provided the fun dament of molecular biological experiment for further studing out the mechanism of CCK-8 protecting lung from injury when endotoxin shock occurs.

Abstract:Analysis on G-banding patterns of the 22 bone marrow cells chromosomes in Yuyi h airless mice and Kunming mice, Two types of G-banding patterns (major and minor-bands) are observed. The karyotype can be distingaished by the characteristic banding patte rns. The G-banding patterns of the two kinds mice lines are identical.

Abstract:Forty-one wild Rhesus monkeys from Zhulin in Simao County in Yunnan province wer e tested for the serum antiboties to SRV by Western Blot. Nine monkeys showed p ostive reactivity to the transmembrane glycoprotein, iegp20. There weren't rea ctivity to the envelope protein, iegp70, and major core protein, iep27 in 41 mon keys. The results showed SRV postive monkeys were existent in wild rhesus monkey s of Simao area in Yunnan.

Abstract:Ascites osteocalcin in H22 tumor cells transplant mice and peri toneal cavity fluid osteocalcin in normal mice were determined. The results sho wed that the concentration of ascites osteocalcin was lower than that in normal controls, at 10 and 13 days post transplant, 77% and 83% lower respectively, P<0.02.

Abstract:Selected hybridoma which secreted steadily anti-human liver McAb were injected a variant sex and age LOU/C-Igk-1b rat to obtain the ascites. The data suggest th e output of the ascites is the peak in 20 weeks and have no significant differe nce in sex. It indicates that the output of the ascites from 3A5 McAb is correla t ed with the age and weight of the rat. The researcher utilizes effectively the a nimal to produce a vast amount of ascites on the basis of the results.

Abstract:150 male Kunming mice at age of 20 days were divided into 5 groups and 150 coupl es Kunming mice in sexul maturity were also divided into 5 groups randomly. The mice in different groups were fed control diet and experimental diets contain di fferent Zine level of 60,70,80 and 90 mg/kg respectively. The results showed that the zinc level in diets have no significant infuluence on weight gain, fee d efficiency and reproductive performance of mice in different groups. There wer e also no significant difference in zinc content in serum, liver, pancreas, kidd ey, tibia and serum AKP in different groups. The results demonstrated that the z inc level in natural ingredient diets can not only satisfy the mice requirement for growth and reproduction, but also make tissue zinc to reach saturation. It i s unnecessary to supplement zinc additive to mice diets.

Abstract:本文对半屏障系统下清洁级Ｃ５６ＢＬ／６小鼠进行剖腹取胎，用触诊法确定妊娠鼠的临产期，剖腹产仔鼠成活率为８９５２％，部腹产和正常产仔鼠出生体重差异不显著（Ｐ＞００５）。用ＩＣＲ母鼠代乳与正常产的仔鼠１～４周龄同期体重比较差异显著或极显著（Ｐ＜００５或Ｐ＜００１），４周龄头长、体长比较无显著差异（Ｐ＞００５）。

Abstract:用二乙基亚硝胺诱发大鼠肝癌，观察其影像学表现，同时与病理学进行对照。结果，３０只雄性Ｗｉｓｔａｒ大鼠中，诱癌过程死亡１３只，余１７只经病理学检查，证实均成功诱发出肝癌灶。癌结节直径为０２～２．５ｃｍ，每个肝脏平均有９±２１个。其影像学特征为：平扫ＣＴ癌结节为略低密度区，增强扫描低密度影显示更加清楚；ＭＲＩＴ１加权像呈等或略低信号，Ｔ２加权像为高信号。认为化学诱导法建立的大鼠肝癌模型具有人肝癌相似的影像学表现，是一种适合于医学影像学研究的理想模型。