Abstract:Objective To study the expression of Bcl-2,Bax and P 53 and its significance in vascular smooth muscle cells and endothelial cells with injured cremaster nerve of the rat.Methods Immunohistochemical SP method was used in this study.Results The expression of Bcl-2,Bax and P 53 in smooth muscle cells and endothelial cells was obvious but the number of positive cells of Bcl-2 was larger than that of Bax and P 53 in smooth muscle cells.In endothelial cells,expression of Bax and P53 maintained at a higher level.Conclusion Cremaster nerve injury could result in proliferation of smooth muscle cells and apoptosis of endothelial cells and might play an important role in dysfunction of microcirculation.

Abstract:目的　对不同单位保存的小鼠肝癌H2 2 细胞株的细胞特性进行比较研究。方法　从三个单位引进小鼠肝癌H2 2 瘤株 ,KM小鼠腹腔传代 ,然后进行染色体、DNA含量、细胞大小比较分析。结果　中国医学科学院药物研究所(北京H2 2 )、山东省医学科学院药物研究所 (山东H2 2 )和武汉大学典型培养物保藏中心 (武汉H2 2 )三个单位H2 2 瘤株的染色体均数分别为 (30 3± 8 9)条、 (33 0± 8 9)条、 (35 6± 10 1)条 ,方差分析表明不同单位瘤株间差异均有显著性 (P <0 0 1) ;直方图分析显示主流染色体范围分别为 2 7～ 2 9、 30～ 32、 33～ 35。武汉H2 2 瘤株细胞面积、长径、短径与北京H2 2 、山东H2 2 比较差异均有显著性。流式细胞术DNA含量分析表明北京H2 2 DNA含量最少 ,武汉H2 2 DNA含量最多 ,统计学分析表明不同单位瘤株间差异均有显著性 ;DNA指数结果表明武汉H2 2 为非整倍体肿瘤 ,北京H2 2 、山东H2 2 为近二倍体肿瘤。结论　不同单位保种和使用的H2 2 瘤株的细胞特性已存在显著差异。

Abstract:Objective To investigate the prevalence of duck hepatitis B virus(DHBV)in two farms. Methods A sampling survey of the duck species of domesticated Anas Platyrhynehos Linnaeus was carried out. 519 blood samples were collected from two farms in Heshun and Lishui towns of Nanhai city in Guangdong province,being from three categories,namely,newly-hatched,growing and full-grown ducks. The Dot Blot Hybredization measurement was to determine the DHBV prevalence. Results The average natural carrying rate of DHBV is 36.0%(187/519)from two farms. 95% confidence interval of the tested blood sample is within the range of 31.0%-44.0%. The differences of natural carrying rate of DHBV are nonsignficant for ducks of different ages,farms or sexes,while the differences of the values of optical density(A)of DHBV-DNA between ages are extremely significant(P<0.01),that is,the values decrease from newly-hatched to full-grown to growing ducks. The A values in Heshun farm samples are higher than Lishui. Conclusions DHBV carried by Anas Platyrhynehos Linnaeus from both farms may be probably derived from different sub-strains.

Abstract:目的　探讨化学发光加强法(ECL)核酸直接标记和检测系统应用于猪的DNA指纹分析的可行性。方法　采用人源α-珠蛋白3'HVR探针，应用ECL核酸直接标记和检测系统，对西双版纳小耳猪进行了Southern杂交DNA指纹分析，获得了最适实验条件。结果　取10μg基因组DNA于30μl反应体系中，加入HinfⅠ或HaeⅢ内切酶3～5U/μgDNA，酶切消化4h，消化液在50V电压条件下4℃电泳20h，采用真空转移法进行脱嘌呤、碱变性、中和及转膜，时间分别为15min、15min及20min，转膜采用20×SSC真空转移1h至hybondN+尼龙膜上，大大提高了转膜效率。以HRP标记探针进行管中杂交，以化学发光法进行检测，室温放射自显影30min，获得了清晰可辨的DNA指纹图。结论　该方法可用于猪的DNA指纹分析，其效果与同位素标记探针的效果基本一致。

Abstract:目的 研究大鼠提睾肌神经损伤后血管平滑肌细胞和内皮细胞的Bcl-2、Bax及P53表达和意义。方法 以免疫组织化学SP法，对大鼠提睾肌神经损伤后的血管平滑肌细胞和内皮细胞的Bcl-2、Bax及P53表达进行研究。结果 支配提睾肌的神经损伤后，Bcl-2、Bax和P53均有表达。在平滑肌细胞中，Bcl-2的表达明显强于Bax和P53，而在内皮细胞Bax和P53的表达强于Bel-2。结论 大鼠提睾肌损伤后平滑肌细胞有增殖现象，而内皮细胞则可能出现过度凋亡，上述改变可能对微循环产生不利影响。

Abstract:目的　对不同单位保存的小鼠肝癌H

Abstract:Swelling at both sides of breast and one side of abdomen were found during nurturing the APP transgenic mice progress.They were diagnosed as breast cancer and chronic myelocytic leukemia by dissection and pathological study.It is proved that the conformity site of exogenous gene in APP transgenic mice is random,the tumor induced by gene conformity site could manifest with different stage of cell growing.

Abstract:目的 研究孕酮对沙土鼠前脑缺血海马受损神经元的保护作用。方法 采用同时夹闭双侧颈总动脉制成沙土鼠前脑缺血动物模型，测定腹腔注射孕酮后海马CA1区锥体细胞层神经元数目的变化。结果 前脑缺血组沙土鼠海马CA1区锥体细胞层神经元数目明显丢失，CA3区和齿状回相应层次的神经元数无明显变化。前脑缺血再灌注后腹腔注射孕酮可使海马CA1区锥体细胞层神经元丢失明显减少。结论 孕酮对脑缺血所引起的神经元损伤具有明显的神经保护作用。

Abstract:Objective To explore a new immunological genetic therapy for glioma with the liposome-mediated transgenic method.Methods We introduced the pLXSN-expressed recombinated plasmid into the glioma cell line C6 of Wistar rat,then we pick up the positive clone by G418 screening,finally,got the expression of B7 gene in C6 cell line with PCR,RNA-slot hybridization.Results With PCR,we obtained 0.8Kb's segment of B7 gene from the genome of the B7-transdued C6 cell (C6pLXSN-B7); with RNA-slot hybridization using B7 as the probe,we found the transcription of B7 gene in the C6pLXSN-B7 cells;with anti-B7 the expression of B7 molecule on the surface of C6pLXSN-B7 cells,on the contrary,there was no expression of B7 in the glioma cells of the control group (C6pLXSN,C6).Conclusion The liposome-mediated B7 transgenic method can lead to the entire integration of B7 gene into the genome of C6 cells and the resulting stable and effective expression of B7 gene.

Abstract:Objective To develop a method of removing harmful gas in air in room of laboratory animal.Methods Based on principle of removing ammonia in industry,a small-sized equipment was produced.Results Simulant experiment indicated,when both fresh air by fan and cycling air by the small-sized equipment were in turn at intervals of one hour,the concentration of ammonia was 12.02ppm in air of laboratory animal room.Conclusion The system can remove ammonia in the room to a certain extent.

Abstract:目的 通过鼠痘病毒抗体的制备摸索鼠源性多抗的大量生产方法。方法 用完全福氏佐剂乳化的鼠痘抗原经腹腔注射免疫10只昆明小鼠，共免疫4次，其间腹腔注射一次液体石蜡。结果 试验中7只小鼠产生腹水，平均每只产10．6ml，最高达26ml，腹水总产量是血清总产量的15倍。同一只小鼠腹水抗体效价在不同采取时间保持稳定，不同小鼠间腹水抗体效价差异较大，在1：320～1：5120之间。腹水通过56℃ 30min灭活处理后，效价没有变化，灭活处理的腹水冻存于-40℃的条件下，6个月内经反复冻融效价基本保持不变，未经灭活的腹水冻存后效价下降明显。结论 用腹腔免疫诱发腹水可得到产量较大，效价较高的特异性抗体。

Abstract:Objective To establish a simple method for germplasm storage of transgenic mice by vitrification.Methods Ethylene glycol(EG) was used as a cryoprotectant for embryo cryopreservation of HBV-C transgenic mice by vitrification.Results The best results of cryopreservation were obtained after using vitrification solution containing 30% EG.The developing percentage of thawed embryos were 81.54% and 69.81% of them developed to expanded blastocysts.Comparing recovered compacted morulae with blastocyst in expanded blastocysts percentage.the former were significantly better than the latter(78.95% vs 56.52%,P<0.05).Different methods of installation of the 0.25-ml plastic straw infulenced the recovery rate.When recovered embryos were transferred into pseudo-pregnant recipients,54.9% embryos developed to live offspring.Conclusion This method may be of practical use for embryos preservation of transgenic mice.

Abstract:Objective To search for a new method to determinate the bone remodeling ability of bone morphogenetic protein(BMP).Methods Self-prepared bovine morphogenetic protein (bBMP)with same weight was buried in the muscles of the behind legs of the mouse.The place which bBMP was buried in was examined with ultrasonography,X-ray and histological section at 3,5,7,14,21,28 day after the operation respectively.Results No new bone was found by ultrasonography and X-ray at 3,5,7,14 day and the signs of new bone appeared at 21,28 day.By histological method a large number of mesenchymal cells were seen in the muscles in which bBMP was buried at 3,5,7 day,and the formation of new cartilage and the new bone at 14 day,then the normal bone developed at 21,28 day.Conclusion The results of B-mood ultrasound and X-ray and histological section were almost the same.So it seems feasible that B-mood ultrasound is regarded as a new method to determine the bone induction ability of bBMP.

Abstract:目的　探讨以输尿管体外结扎(Extracorporeal ligation of ureter，ELU)取代肾移植受体大鼠原肾切除的可行性。方法　观察ELU对非移植组(组1)大鼠的影响；比较ELU(组2)与原肾术中切除(组3)及原肾术后切除(组4)对肾移植受体大鼠存活及术后早期肾功能的影响。结果　组1大鼠左侧ELU后15d内均存活良好，右侧ELU后均在3～4d内死亡；组2ELU后第3dSCr明显低于术后第3d；组3的SCr及组4再次手术切除原肾后第3d的SCr〔(46.6±4.4)μmol/Lvs(68.1±7.3)μmol/L，P＜0.001及vs(54.3±4.6)μmol/L，P＜0.005〕；术后15d内组2大鼠存活率明显高于组3及组4(100%vs60.0%及vs57.2%，P＜0.05)；病理检查显示ELU后无漏尿、感染并发症。结论　输尿管体外结扎技术方法简便、效果可靠、创伤小，可取代肾移植受体大鼠原肾切除。

Abstract:目的　建立犬减压病模型。方法　采用不充分减压法诱发犬减压病模型。结果　狗在0.6MPa压缩空气环境暴露60min，以(0.07±0.02)MPa/min速率减至常压后可诱发犬减压病。结论　0.6MPa组发病率(83.3%)明显高于0.2MPa组和0.4MPa组(16.7%和41.7%，P＜0.05)。

Abstract:The construction of laboratory animal facilities which involves many specialties is a complex project.For improving the research work there,as well as the reliability of safety test on foods,cosmetics,and other health products in the limited clean animal laboratories,Chinese Academy of Preventive Medicine (CAPM) has built a clean animal facility for more research and inspection projects,through selecting the construction team openly and publicly,consulting with laboratory animal experts,and supervising the constructive quality.And in CAPM an Experimental Animal Laboratory was established which is available for several institutes,achieving its aims for conforming to the National Standard,satisfying the needs,saving energy,reducing manpower,using the facility efficiently and decreasing the maintenance expenses.The management is also changed from the lower standard and dispersion to the higher standard and centralization.CAPM has been getting big benefit from the change of managemental model.

Abstract:This article mainly introduces how to use the technology of B/S,ADO,ASP to construct laboratory animal resource information database,which would have either the function of retrieval services and paper publication or the ability of electronic buisness on internet and web.

Abstract:Animal welfare issues are facing each scientist in biomedical research.These problems are complex,involve a diversity of views,and will not disappear in the future.Biomedical scientists need to be knowledgeable about the issues,develop sensitivity to the diversity of thought about these issues,and take an active stance toward animal welfare.Scientists must go beyond compliance and use skills to maximize well-being of experimental animals.It is essential that scientists should advocate animal well-being and adhere to appropriate guidelines for animal care and use when conducting research with laboratory animals.