Abstract:目的　研究与ARDS密切相关的几个生理指标。方法　KM小鼠动物血pH、PaO2 、PaCO2 由美国NO VA血气分析直接测定 ,PQ中毒LD50 以寇氏法计算。肺系数由湿重除以体重乘 10 0 %得出数值。结果　KM小鼠正常动脉血pH值为 7 36 0 5± 0 0 6 7,PaO2 (kPa)为 12 47± 1 34 13,PaCO2 (kPa) 5 2 6± 0 4949;肺系数 (% )为 0 6 6 6 2±0 0 82 ;百草枯 (paraquat,PQ)中毒LD50 为 93 86 6mg kg。结论　以上所测定几个数据至今尚未见报道 ,我们把测定分析结果公开发表 ,填补KM小鼠理化常数方面的空白 ,为研究临床ARDS以及其他呼吸系统疾病提供极有意义的参考数据

Abstract:Objective To select a full\|nutrient diet for laboratory animals which has good quality as well as lower price through comparing the grouth effect on Wistar rats from different diets produced in Beijing.Methods Wistar rat A,B and C from different units were divided into 4 groups fed with 4 kinds of full\|nutrient diets a,b,c and d.By weighting each rat and the diet in each cage every week the increased weight of 4 groups of rats and the day\|average consumption of 4 diets in each cluster were compared.Results The average increased weight of 4 groups in the same cluster of Wistar rats were no significant difference (P>0.05),but the day\|average consumption of 4 diets was different.Conclusion there is no significant difference between the 4 diets produced in different companies for the growing Wistar rats of the 4 groups in same cluster. The day\|avergae consumption of diet d is the highest,and that of diet c is the lowest.

Abstract:Objective To evaluate the reproduction and growth of the Kunming(KM) mice in Lanzhou Institute of Biological Products(LIBP), as a part of the study on KM mice's biological features standardization in People's Republic of China.Methods 170 pregnant female mice were chosen from the workshop's population and the growth of the baby mice was evaluated by measuring the litter weight of 3, 7, 10, 14 days and the body weight of 3, 4, 5, 6, 8, 10, 12, 14*!weeks after birth.140 pairs of mature mice were used. Of which 136 pairs were mated within 70-75 days. The reproduction of these mice were evaluated by measuring the producing rate in 30 days after mating, the average litter number per birth, the survival rate of weaning and the time of between births.Results The average weight of newborn litter is 20g; by 14th day, the average weight of litter is 67.4g. By 10th week, the male mice weigh 39.22g,showing body-mature, and the female mice weigh 32.45g, above 90% of body-mature. The producing rate within 30 days after mating is 81.6%. The producing average of 1-5 births is above 10, the producing average of 6-10 births is about 7. The average weaning rate is 84%.Conclusion The results above could provide a reliable data for the standardization of KM in China.

Abstract:Objective In order to master the diagnosis method of vitamin D intoxication by studying vitamin D of content diets of laboratory rats and mice.Methods BALB/c mice were fed with different vitamin D content diets as experimental group and control group, pathological observations were made and blood phosphorus and blood calcium were determined. Results The rate of myocardial calcareous deposit is 53.5% in the experimental group and 0 in the control group. Blood calcium value of the former is 1.6 times that of the latter; blood phosphorus value shows no significant difference between two groups.Conclusion The decreasing of weaning rate, the myocardial calcinosis and the rising of blood calcium value in laboratory rats and mice are caused by superfluous vitamin D intake.

Abstract:目的研究从S180获得的克隆细胞株S2D9,接种4个不同品(种)系小鼠建立肿瘤动物模型的某些基本生物学特性.方法由KM小鼠腹腔传代的S2D9克隆细胞,用GKN稀释成不同浓度,接种KM、DBA/2、615、C57BL/6小鼠右腋皮下及腹腔,观察小鼠最早出现可见肿块、腹水时间及小鼠生存时间;不同品(种)系小鼠经皮下接种S2D9细胞,建立肿瘤模型,从接种后第5日,每日观察肿瘤生长情况,测量肿块直径(cm),绘制肿瘤生长曲线;S2D9克隆细胞株接种BALB/c小鼠皮下,取接种第7日肿块,经石蜡切片,HE染色,观察病理结果;不同品(种)系小鼠皮下接种S2D9瘤细胞建立的动物模型,经腹腔注射环磷酰胺(CTX)或顺胺氯铂,观察S2D9克隆细胞肿瘤模型对CTX或顺胺氯铂治疗的敏感性.结果S2D9克隆细胞在615及DBA/2小鼠的皮下较KM及C57BL/6小鼠容易生长肿瘤;在KM及DBA/2小鼠腹腔较C57BL/6及615小鼠容易长出腹水;皮下接种KM、C57BL/6、615及DBA/2四个不同品(种)系小鼠,第12天瘤重分别为(2.3±0.9)g,(0.8±0.2)g,(2.0±0.2)g及(1.9±0.1)g;S2D9瘤细胞接种4个不同品(种)系小鼠制备的肿瘤模型,用CTX治疗,抑瘤率分别为80%(615),75%(C57BL/6),68.4%(DBA/2),65.2%(KM);用顺胺氯铂治疗的抑瘤率分别为75%(615),73.6%(DBA/2),62.5%(C57BL/6),65.2%(KM).可见S2D9细胞与615小鼠建立的模型对CTX或顺胺氯铂治疗最敏感.结论S2D9克隆细胞仍具有较强致瘤性,但在细胞均一性、接种小鼠生长肿瘤一致性方面比原代细胞好,对化疗反应更敏感,克隆细胞易于进行质量控制,因而推广使用后,有利于肿瘤动物模型复制的一致性和可重复性.

Abstract:Objective To investigate the spontaneous skin carcinoma of Japanese big-ear rabbit. Methods Pathological histology HE staining and electron microscope were adopted.Results Under optical microscope, the carcinoma cells arranged in nest dwelling pattern with empty and understained cytoplasm. Big nuclei and pathologic mitosis could also be seen often. Under electron microscope, the carcinoma cells arranged tightly with desmosemes between some cells. Glycogen and tonofibril in cytoplasm were common,with little other organelle seen. Nuclei of carcinoma cells were big and various in size with irregular karyomorphism.Conclusion The skin carcinoma is spoutaneous sweat gland carcinoma.

Abstract:Objective To measure the organ weight and organ coefficient of SPFminiature pig.Methods 60 day old SPF miniature pigs ,10 each of both male and female, were used and compared in this experiment.Results Between male and female,the brain and lung weight showed very significant difference (p<0.01),the body weight ,heart weight and liver weight had significant difference (p<0.05); weight differences of spleen,kidney ,suprarene and stomach were little(p>0.05).The organ coefficient of the brain and lung between the male and female had very significant difference(p<0.01), male's being higher than female's, but no difference was seen in the other organ coefficient(P>0.05).Conclusion The above data could be useful in the biomedical researches.

Abstract:目的研究与ARDS密切相关的几个生理指标.方法KM小鼠动物血pH、PaO2、PaCO2由美国NOVA血气分析直接测定,PQ中毒LD50以寇氏法计算.肺系数由湿重除以体重乘100%得出数值.结果KM小鼠正常动脉血pH值为7.3605±0.067,PaO2(kPa)为12.47±1.3413,PaCO2(kPa)5.26±0.4949;肺系数(%)为0.6662±0.082;百草枯(paraquat,PQ)中毒LD50为93.866!mg/kg.结论以上所测定几个数据至今尚未见报道,我们把测定分析结果公开发表,填补KM小鼠理化常数方面的空白,为研究临床ARDS以及其他呼吸系统疾病提供极有意义的参考数据.

Abstract:Objective To detect the ectoparasites in the conventional Golden Hamster of our institute.Methods 3 methods(pluking,pasting with the transparent glue tape and scrapping of dander) and microscopic examination were used.Results Notoedres was detected on the Golden Hamster' cuticle in trouble with dermatitis, and the infection rate was 4.4%. Conclusion Avoiding the infection of ectoparasite and endoparasite, the Golden Hamser should be upgraded to the level of clean animals by means of improvement of feeding equipment service,feeding environment condition,feeding method,seed selecting and breeding,and so on.

Abstract:Objective To establish a nest PCR method and its application for testing 16S rDNA of Clostridium Piliforme. Method The nest PCR condition was modified on the basis of Goto's report. Results The outside pair of primers can lead to the synthesis of a 625*!bp DNA fragment while the inside pair of primers can lead to a 196 bp DNA fragment. Both amplified fragments are exactly the same size as Goto 's report. The 196bp DNA fragment has been demonstrated to be the specific sequence conserved in Clostridium Piliforme in our experiment. Our PCR assay is of great sensitivity, detecting even 2 organisms in positive sample. The sequence of 625 bp DNA fragment amplified in our positive control is 99% homologous to both the MSK and RJ strains as reported by Goto. Conclusion The nest PCR assay established in our laboratory can be used to amplify specifically 16S rDNA of Clostridium Piliforme. 5 liver samples each from mouse, rat, golden hamster, 10 liver samples each from gerbile, guinea pig bred in conventional environment and 1 liver sample infected with MHV3 from mouse bred in clean environment have been tested for Clostridium Piliforme by this method. No latently infected animals have been identified among the 36 samples.

Abstract:Objective\ To establish SPF and clean rabbits colony and manufacture base,and setup a technique to breeding and reproduce clean rabbits in the clean environment for the improvement of quality of experimental rabbits.Methods\ We use hysterectomy,tear the uterus in the isolator,let the pups anabiosis.The young were kept in germfree isolator,and were fed with artificial milk by hand,becoming germfree rabbits.5 kinds of microorganism were inoculated,one month later,the feces and serum of two rabbits were examined for microorganism and virus to confirm if SPF animal standard met.Results\ 52 specific pathogen\|free(SPF) rabbits have been obtained with the detection and confirmation of authorized institution.Conclusion\ We transfer baby rabbits to SPF and clean facility,in which they grow and reproduce well.It indicates that this technique is useful, repeatable and maneuverable.

Abstract:Objective To establish the technical data of transgene and cryopreservation. Method a)Five different genes were microinjected into ova, totaling 1864 1\|cell ova injected and 1283 2\|cell ova transferred. Finally we produced 20 transgenic mice. b)We got the optimum dosages of hormone for superovulation by five mouse strains injected with PMSG and HCG. Also we got average data of embryo superovulation of different strains. c)The embryo cryopreservation was produced by the normal method and glasses method. Results and Conclusion a)The integration rate of transgenic was 16.16% and the efficiency of gene transfer was 1.56%. b)The average superovulation number of different strains was 15.6 for C 57BL/6J (Japan) strain; 19.6 for C 57BL/6J; 21.4 for BDF1 (Japan) strain; 14.8 for C3H strain; 31 for KM strain. c)The ratio of the pups born after cryopreservation of mouse embryo was 30%-31%.

Abstract:Objective To research and develop a management information system which can be used in experimentation animal breeding farms.Methods and Results\ Microsoft Visual Studio 6.0 is used as the major development platform. The system has achieved high stability while being applied with COM technology. HTML/ XML, WEB has also been applied to enhance the ease of the operation as data formatting and reporting. All the reports and data files can be edited directly inside Microsoft Office 2000, and can be further published on web sites or faxed out.The system is featured for its Web tech ology, new concept search engine and view technology. The usability of the whole system is greatly increased. Windows OLE technology is also applied to achieve data drag and drop,and software disks and data calculator help to minimize the manual processing by operators.Conclusion The Management Information System is very suitable for the management work of experimentation animal breeding farms. It is worth recommending and deploying widely.

Abstract:Paper reported a better method of captive breeding and management of macaque in large cages according to macaque's biological characteristic and environmental conditions,that is, feeding on time,adjusting food nutrition,adding extra trace element in pellet,monitoring environmental temperature and relative humidity,preventing and curing diseases regularly in macaque.And the macaque fertility rate has been raised by means of the method.

Abstract:Objective To find out the reles and obtain the reference data in the designs of barrier animal facilities.\ Methods The 24 charts of barrier animal facilities were analyzed in this study. Results The various facilities occurrence and their mean area were calculated in the barrier animal facilities. Many essential rooms there and their pecentage were offered:clean corridor 7.5%, dirty corridor 7.3%, first dressing room 1.6%, second dressing room 1.1%, bathroom 0.9%,air shower room 0.6%,buffer 4.2%, quarantine room 3.6%,breeding room or experimental room 39.8%, preparation room 5.0%, store room 3.3%, washing and sterile room 10.4%, machine room 10.8%, office 3.9%. The size of both contamination room and incinerator chamber varied with different conditions.Conclusion The data above can be useful in the designs of barrier animal facilities.