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2002(3):131-134.
Abstract:目的 了解东方田鼠的消化特征和科学,合理配制其饲料。方法 对东方田鼠(Microtus fortis)胃肠进行解剖组织学观察,以及对三组不同营养水平饲料氨基酸消化能力进行测定。结果 东方田鼠的胃可分为前胃和后胃,后胃除腺体之外的粘膜为复层扁平上皮组织,盲肠特别大,长,盲肠与肠道的比值[盲肠长度/(大肠长度 小肠长度 盲肠长度)]为0.18。盲肠的粘膜层,粘膜下层和肌肉层明显比其他部分的肠壁薄;配方Ⅰ氨基酸平均转化率为67.56%;配方Ⅱ为57.48%;配方Ⅱ为57.48%;配方Ⅲ为76.75%。结论 纤维素的含量会影响东方田鼠氨基酸的体内转化率。
2002(3):138-140.
Abstract:目的 研究合成HBcAg多肽干预灰旱獭感染HBV的作用。方法 合成筛选的HBcAg多肽Ⅰ、Ⅱ二个片段,选对HBV-DNA产生血清反应的10个灰旱獭,均5组实验,第一组HBV-M全阴性单注HBcAg多肽Ⅰ、Ⅱ。第二组HBV-M全阴同注多肽Ⅰ、Ⅱ和含HBV-DNA的人血清,第三、四组为HBcAb^ 组注多肽Ⅰ、Ⅱ。第五组为HBV-M全阴注生理盐水对照组。结果 第一组注多肽后28dHBcAb^ 伴有酶血症,190d时出现抗-HBs^ 持续存在210d。第二组注射后观察305dHBV-M仍为全阴性。第三、四组注后随访150d和360dHBV-M均为阴性。第五组(对照组)无任何反应。结论 选择的多肽片段有阻断HBV感染和使HBcAb转阴的作用。即抗-HBV的作用。
2002(3):141-143.
Abstract:目的 分析D-半乳糖衰老模型大鼠主要脏器自由基代谢状况。初步探讨该模型的羰基毒化机理。方法 以D-半乳糖头颈部皮下注射,制造大鼠衰老模型,检测相应脏器组织细胞及线粒体丙二醛,超氧化物歧化酶,过氧化氢酶,并进行模糊综合分析。同时对相应脏器细胞蛋白质的羰基毒化进行初步鉴定。结果 发现该模型鼠脑胶质细胞增生(形成胶质小结),肝脂肪变性,胃肠炎性细胞浸润,粘膜糜烂及其衰老改变;发现D-半乳糖致大鼠衰老与线粒体丙二醛升高,超氧化物歧化酶及过氧化氢酶降低均相关,相关程度为45%,与细胞丙二醛,超氧化物歧化酶,过氧化氢酶亦相关。相关程度为40%〈羰基化鉴定发现,D-半乳糖模型鼠的脑,胃,肝细胞蛋白质有显著的羰基化。结论 初步认为D-半乳糖致大鼠衰老可能是通过羰-氨反应,形成交联产物,通过羰基毒化机制致使大鼠体内蛋白质功能丧失,进而引起一系列的衰老改变,交联产物进一步降解可生成丙二醛等不饱和醛酮中间产物,因而D-半乳糖衰老模型大鼠各主要脏器细胞质或线粒体丙二醛浓度升高。
LIANG Chongli , LI Haishan , ZHAO Wenxing , LI Shurong , SHEN Yi , GUO Hai , CHEN Zhilong
2002(3):144-146.
Abstract:Objective To investigate the curative effect of ethanol extract from propolis (EEP) in phthiriasis of animal.Methods Pharmacodynamic screening test and curative effect test of EEP in sucking lice of rats were done at four different concentration (5g/L, 7 5g/L, 10g/L and 15g/L).Results 10g/L EEP is best ( P <0 01) to kill sucking lice of rat,and to treat phthiriasis of rat.Conclusion Propolis is a natural,cheap,nonpoisonous and easy to gain agent for prevention and treatment of animal phthiriasis.
2002(3):151-152.
Abstract:目的 提高热原试验用兔的测温中选率。方法 以体温性能优良稳定的实验家兔为亲本进行初始代交配,再从仔一代中选取理想的种兔,结果与结论 经过长达五年的体温选择和科学繁育,其繁殖性能与体温中选率明显优于以往的传统方法,平均产仔数为8.3-8.9只,育成率85.0%-88.5%,测温中选率1(38.0℃-39.5℃)78.9%-83.1%中选率2(38.5℃-39.0℃)60.0%-61.5%。
TANG Baizheng , LIU Xing , MA Yadong
2002(3):153-156.
Abstract:Objective To provide basic data of the HSF 1 knockout mice's organ weight and the organ coefficients.Methods 50 HSF 1 knockout mice(male 21,female 29) were chosen for this experiment. Reaults and Conclusion\ The spleen,kidney coefficients had significant difference(\%p\%<0.05) between male and female, the coefficients in the stomach and brain showed very significant difference(p<0 01),but no differences were found in the heart,liver and lung coefficients.
2002(3):157-160.
Abstract:目的 复制具有中医“肝郁”表现的子宫内膜异位症大鼠模型。并进行病理观察。方法 采用手术移植法加用肾上腺素皮下注射法复制模型,取动情期大鼠子宫内膜片段移植于卵巢周围或腹壁内侧面,3-4周后再以0.1%肾上腺素0.15ml/只,作皮下注射,每周一次,共4次,3周后,“肝郁”模型即成,对照组只作腹壁切开术,不作内膜移植。结果 移植物大体呈小囊状,色白透明或暗红,显微镜下小囊具有子宫内膜的基本组织结构,模型动物具有中医“肝郁”的临床症状及其肾上腺的病理改变,结论 造型方法简便易行,周期短,成活率高,有利于中医药研究应用。
2002(3):163-165.
Abstract:目的 建立小鼠肺支原体抗体ELISA检测法。方法 采取与国外试剂盒比较的方法。结果 符合率达100%,检测9个单位送检的184份普通级小鼠血清,肺支原体抗体检出率为97%,清洁级小鼠血清86份,3份为阳性(3.5%)。检测92份SPF级小鼠血清,均为阴性,具有特异性强,灵敏度高,结果判定明确,可靠等优点。结论 建立的ELISA法是一种比较准确的监测方法。
BA Caifeng , LIU Xuezheng , QIN Shujian
2002(3):171-173.
Abstract:In the course of the construction of SPF animal laboratory in Jinzhou medical college, the installation of energy recovery and the heating technique of water derived hot pump was used in order to lower the cost and running expense and save energy consuming.In summer, the heat produced by air conditioning refrigeration was discharged by cooling tower.In winter, heat energy was took away from heating system by the technique of hot pump reverse circulation.The refrigerative and heating capability of air conditioning was automatically regulated by electric three way valve.On the premise of insuring the air conditioning parameter, energy was saved and therefore the running costs were cut down.
XIAO Wei , MA Yun , FU Jiangnan
2002(3):176-179.
Abstract:At recent years, chronic renal failure(CRF) animal model has been successfully induced in many animals such as swine, guinea pig, rabbit, cat, sheep, dog, rat, mouse, \%etc\%.Used methods include surgery, freezing, electric coagulation, ligation, drug, immunity and so on.Every method has its advantages and disadvantages.Using reasonable models may make it better to carry on the study on the laboratory and clinic of CRF.So the article reviews the principle, method, character of CRF animal models, which are expected to provide some reference to the therapy and study of CRF.
2002(3):180-182.
Abstract:This article would discuss the factors affecing the results of animal experimentation in three ways.Experimental animals are the acted objects, and the final expressed main body of outside combinations. And outside combinations act through animal metabolism.The difference of animal race and individual influences the metabolism of outside combination in the animal bodies and, therefore, the action of outside combinations.The environmental factors not only affect the states of experimental animals, but also act on outside combinations.The path and the way through which outside combinations enter the animal bodies influence the metabolism and expression of outside combinations in body.
2002(3):183-185.
Abstract:对封闭群灰仓鼠按实验动物标准的要求进行系列实验动物化研究,简单了该鼠饲养繁殖条件。繁殖及主要脏器系数特征,生长发育,血常规和血液生化值特点,基础代谢特点及体温调节,冬眠特性和生化遗传检测测定(近交系)等。并就灰仓鼠在鼠疫,包虫病,耐饥耐渴,抗凝血剂和雄性不育剂-α-氯代醇等动物模型方面的应用情况进行了探讨。
2002(3):186-189.
Abstract:迄今为止,人骨肉瘤仍是临床上较常见的难以彻底根治的疾病,利用骨肉瘤动物模型对该疾病进行研究,是进一步探索其病因和治疗方法的重要手段之一。本文对骨肉瘤动物模型的建立方法。模型特点及其应用方面进行综述。
2002(3):190-192,F003.
Abstract:近年来在动物体内发现了大量的含硒蛋白,本文对最新发现的硒蛋白种类,功能及其研究现状进行了较详细的概述,研究表明,硒营养状态的变化通过广泛的生化途径来调节动物的生理功能,随着硒蛋白的不断发现,将使一些以前不能与硒缺乏联系起来的临床问题得以明确。