Abstract:目的　研究不同月龄Wistar大鼠肝Ⅰ、Ⅲ型胶原的形态计量的差异。方法　以苦味酸天狼星红———偏光显微镜及Mias 2 0 0 0真彩色图像分析仪对各鼠龄组 ( 3、13、2 5、33月 )肝门管区及窦周隙Ⅰ、Ⅲ型胶原之灰度、面积比及体密度进行了形态计量学测定。结果　窦周隙Ⅰ型胶原之面积比、体密度在 33月龄组分别大于 3、13、2 5月龄组 (P <0 0 5 ) ,其平均灰度 2 5月龄组明显深于 3月龄组 (P <0 0 1) ,窦周隙Ⅲ型胶原之平均灰度 2 5月龄组分别深于 3月龄组 (P <0 0 1)及 13月龄组 (P <0 0 5 )。结论　 33月龄组窦周隙Ⅰ型胶原数量分别多于 3、13、2 5月龄组 ,2 5月龄组窦周隙Ⅰ型胶原平均含量明显高于 3月龄组 ,2 5月龄组窦周隙Ⅲ型胶原平均含量分别高于 3、13月龄组。

Abstract:Objective To investigate biological characteristic of hepatic progenitor cells from fetal liver of rat on ED13.5 days and establish the foundation for liver cell transplantation. Methods Fetal liver cells were isolated by enzymatic digestion. Different influence on cellular biologic action was observed under various cultured conditions and liver specific markers of cells were detected by immunocytochemistry. Results Fetal liver epithelial progenitor cells proliferated on primary rat embryonic fibroblast (PREF) feeder better than non-feeder and were kept undifferentiation. Cell differentiation marks suggested that fetal liver epithelial progenitor cells contain bipotential cells on ED13.5 d.Conclusion There retains hepatic stem cells in rat fetal liver on ED 13.5 d and PREF can be used for amplification of fetal liver epithelial progenitor cells in vitro.

Abstract:Objective To study the apparent intestinal absorption and true intestinal absorption for calcium and phosphorus in different dietary levels of calcium and phosphorus as well as the requirement of calcium and phosphorus by adult laboratory beagal dogs.Method Digestive test and nitrogen balance test were applied. Results Calcium excretion in feces increased obviously and the quantities of calcium absorption were no significantly different when dietary calcium level ranged from 0.52% to 1.02%. Phosphorus excretion in feces increased obviously and the quantities of phosphorus absorption were no significantly different when dietary phosphorus level ranged from 0.39% to 0.87% except 0.39%. There was a positive calcium and phosphorus balance in all test groups. Conclusion 0.52% dietary calcium level and 0.39% dietary phosphorus level are optimal for adult beagle dogs.

Abstract:目的　观察雌激素和胆酸负荷对妊娠大鼠肝胆功能的影响。方法　将 48只近交系Wistar大鼠随机分为 3组 ,试验 1组 :于妊娠第 16天 ,颈后皮下连续注射 5天苯甲酸雌二醇 ,5mg (kg·bw) ;试验Ⅱ组 :于妊娠第 16天尾静脉连续注射 5天游离胆酸 ,2 4mg (kg·bw) ;对照组 :以等量的生理盐水颈后皮下连续注射 5天。所有动物于肝胆汁收集前后采血 ,分离血浆。收集羊水和胎鼠血浆 ,测定血浆、羊水和胆汁中总胆酸 (TBA)、总胆红素 (TBiL) ,丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、碱性磷酸酶 (ALKP)、γ 谷氨酰转肽酶 (GGT)、总胆固醇 (CHOL)各值 ,并作统计分析。结果　雌激素试验I组与对照组相比 ,胆汁收集前后母鼠血浆中TBiL、AST显著升高 ,CHOL显著下降。胆汁中TBiL、AST、ALKP、CHOL显著升高 ,TBA显著下降。胎鼠中TBA和对照组相比显著高 ,TBiL下降。试验Ⅱ组与对照组相比 ,胆汁收集前后孕鼠血浆中TBA显著升高 ;胆汁收集前ALKP、CHOL下降。胎血中AST显著升高。结论　雌激素可以引起母鼠的肝内胆汁淤积 ,使胎鼠血TBA升高 ,但不直接损伤肝细胞。胆酸也可导致母鼠肝内胆汁淤胆 ,也不直接损伤肝细胞 ,但对胎鼠血胆酸没有直接的影响而影响胎盘功能。

Abstract:目的：研究停经期猕猴外部体征、表情行为及体内性激素水平变化规律。方法：根据性皮肤规律性变化周期，观察停经期猕猴外部体征及表情行为变化，并选择时机拍照录像，采用放射免疫法检测其体内雌、孕激素变化规律。结果：8只停经期猕猴性皮肤规律变化周期天数平均为33天；停经期猕猴性皮肤浅淡期与正常猕猴经前期体征及行为基本一致，性皮肤红肿期与正常猕猴卵泡期基本一致；性皮肤浅淡期阴道涂片细胞学变化与正常月经期猕猴经前期基本一致，性皮肤红肿期与卵泡期基本一致；二猴E2在停经期性皮肤规律变化过程中有一明显分泌高峰，大约在指定周期的11天左右，随后在周期第19天左右亦出现一上升段；二猴体内孕酮在停经期性皮肤规律变化过程中有一分泌高峰段，大约在指定周期的19天。结论：停经期猕猴行为、体征及体内性激素水平变化规律与其正常月经周期时基本一致。

Abstract:目的　研究大豆异黄酮类对去卵巢大鼠性激素、血脂、腹部脂肪的作用及机理。方法　用大豆异黄酮、金雀异黄酮大剂量、小剂量及雌激素灌肠胃 6周后观察药物对大鼠甘油三脂 (TG)、总胆固醇 (TC)、腹部脂肪及雌二醇 (E2 )、孕酮 (P)、睾酮 (T)、泌乳素 (PRL)、促卵泡生成素 (PSH)、促黄体生成素 (LH)的影响。结果　模型大鼠去卵巢 7周后血清TG( 3 94± 0 2 )、TC( 4 17± 0 2 5 )水平、腹部脂肪重量 ( 15 2 4± 3 95 )、体重指数 (BMI=体重 (g) 身长 (cm) 2 ) ( 0 6 77± 0 0 41)较假手术组增加 (P <0 0 5 )。雌激素 (E2 ) ( 1 2 6± 0 76 )、孕酮 (P) ( 2 8 13± 17)、睾酮 (T)( 0 2 6 7± 0 2 7)较假手术降低 (P <0 0 5 )。服用雌激素、大豆异黄酮、金雀异黄酮组、雌二醇 (E2 )增加 ,腹部脂肪重量下降 ,体重指数、血清TG、TC下降 (P <0 0 5 ) )。结论　大豆异黄酮、金雀异黄酮具有降低去卵巢大鼠腹部脂肪、血清甘油三脂 (TG)、总胆固醇 (TC)的作用。其原因可能是由于能使去卵巢大鼠血清E2 增加。

Abstract:Objective To study the mechanisms of pathogenicity of %Staphylococcus aureus%(SA). Methods Experimental strains of %S.aureus% were SA1800 and SAm0109. After inoculating with the bacteria, the clinical symptoms and pathogenic changes of experimental mice were examined. Results Obvious invasion of the bacteria was found. The LD 50 of SA1800 to experimental mice was 10 -4.1/0.2 ml, and the LD 50 of SAm0109 to experimental mice was 10 -5.1/0.2 ml. Conclusion It was a good animal model for studying the pathological mechanisms of %S.aureus% and the development of its vaccine.;

Abstract:Objective To compare the three models of renal tubulointerstitial injury. Methods Gentamycin ip.(GM), unilateral ureteral obstruction(UUO), cyclosporine A(CsA) fed by oral were used to make the renal tubulointerstitial injury, then to detect urine biochemistry and blood biochemistry, and observe nephritic pathology. Results The nephridium indices of the models were all significantly higher in comparion with the control group(%p%<0^01). Urine protein and NAG enzyme of the GM model were significantly higher than the control on the 7th day, however, no significant difference was observed on the 15th day. Urine protein and NAG enzyme of the UUO model were significantly higher than the control on the 7th and 15th day, whereas urine protein of the CsA model was lower (7d %p%<0.05, 15d %p%<0.01). SCR and BUN of the three models were significantly higher than the control, whereas TP and ALB of the three models were significant lower than the control, There's no significant difference between the GM model and the UUO model on 15th day. Observation under light microscope showed inflammatory cell in interstitium, outspread of renal tubules, fibrosis in interstituium of the UUO model. The morphological changes were much slighter in the CsA model. Conclusion The three methods all could cause different level of renal tubulointerstitial injury. Fibrosis in interstitium of the UUO model was like the sign of clinical nepherosis. The UUO model was an ideal model for the study of the renal tubulointerstitial injury. The GM model seriously generating renal tubulointerstitial injury on the 7th day could be used for the acute model. However, the CsA model was not equal to the GM and the UUO models.;

Abstract:目的：通过对华南猕猴红细胞ABO血型研究，为建立此类动物的输血模型提供参考依据。方法：运用单克隆抗—A／抗—B和人源抗—A／抗—B血清(效价为1：256)进行直接凝集试验，采用Terao等报道的吸收放散试验，从30只猕猴中检定类人AB0血型。结果：直接凝集试验未能检测出猕猴红细胞上的ABO血型物质。吸收放散试验筛选出A型猴3只(10％)、B型9只(30％)、AB型3只(10％)，其它15只(50％)未检出(暂定为非A非B型)。结论：华南猕猴的红细胞上具有较弱的类人ABO血型物质。

Abstract:目的：建立二乙基亚硝胺(Diethylnitrosamine，DEN)诱发兔肝癌模型，获得兔肝细胞癌组织。方法：30只雄性日本大耳白兔，在1／6肝切除后，以20mg/L的DEN水溶液代替饮水4周，每周5d，此后将DEN的浓度降至10mg／L，直至濒死或触诊发现肝肿物。实验过程中每间隔2个月检测一次血清AST、ALT、γ—GT和AFP值，所有动物最终均进行解剖检查，可疑脏器做组织学检查。结果和结论：21只动物在给DEN后22个月内中毒死亡或濒死期处死，仅有1只动物于第22个月发现肝癌，5只存活至23个月的动物均诊断出肝癌，6例肝癌中，5例为高分化多发性肝细胞癌，1例为低分化肝细胞癌，3例发生肺转移，1例肾转移。所测血清指标与肝癌发生、发展未见明显相关性。

Abstract:The fetal bovine serum (FBS) has been widely used in biomedical research. This paper is concentrated on the ethical and scientific problem on the production and use of FBS, reduction of FBS and development of alternatives to FBS.

Abstract:In the body complement system is an important effect and effect-enlarging system with many biologic functions. Complement content in guinea pig serum is higher than any other laboratory animals. This text would review some research advances of guinea pig complement system in molecular biology.

Abstract:In order to promote the development of acupuncture theory, the study of meridian and acupoint construction and biological character of rabbit on the aspects of anatomy,physiology and others is reviewed.Through the analysis of correlated research data and veterinary literature in recent twenty years, the rabbit has meridians and acupoints on the surface of body which are similar to those of human beings. Along the meridians,the skin corneum is thin,nerve endings and mast cells are abundant. The acupoints have three-dimensional structure and their biological character is much in common with human's.

Abstract:The itch-associated responses of laboratory animals arisen from external stimulation were introduced in this article. The degrees of responses could be quantitatively evaluated by the analysis of skin nerve activities of laboratory animals and electrophysiologic parameters, which is useful for studying the effect of itch-resistant drugs.

Abstract:In this article,the authors review the experimental animal models of IUGR that have been established up to present, and compare several kinds of familiar experimental models.

Abstract:SHRSP rat is the model animal for spontaneously hypertension and stroke, and is cultivated through selective mating and secondary inbred. The incidence of cerebral apoplexy is higher than 90%, and the animals would display cerebral hemorrhage or cerebral infarction, the peri-focus blood vessle walls thickening and in hyaline degeneration, increased renin activity and molecular weight. And raised blood pressure is a prerequisite to cerebral apoplexy occurrence and development. The HFC burden test showed 5.9-9.7 times higher concentration of total cholesterol, severe nephrosis and appearance of renal glomerulus foam cells. The foam cells rose with hypertension continuation. The permeability of lipid rose as the capillary of kidney was damaged following hypertension, and hyperlipemia facilitated the development of nephrosis. The sclerosis of renal glomerulus was accompanied with increased foam cells, whose characteristic of the distribution pattens of human and rat was indentical. It is suggested that SHRSP rat can be used as the model for human disease.

Abstract:Between 1970 and 2001,eleven coat mutants of Mongolian Gerbil arose in scientific laboratory and pet population.Among them,eight coat colors and their genetic mechanism were detailed in this article.The wild system includes two loci,one is A locus(coat color is agouti),the other is a locus(coat color is non-agouti or black).C system includes c locus (albino),c+h(acromelanistic albino),c chm(chinchilla medium).The white spotting mutant is controlled by Sp locus.g locus appears to a gray coat color, and p locus seems to be master of pink-eyed dilution.The hairless phenotype is only elementary introduction. No reports are on the molecular mechanism in the coat colors of Mongolian gerbil,hitherto.The gerbil is a kind of multifunctional laboratory animal;therefore studying on the field of the gerbil coat colors has wide prospects.