Abstract:目的　检查了解SHR、SHRsp、WKy三品系大鼠血液生化的参数。方法　每个品系分别取年轻大鼠(12周龄 )、年老大鼠 (2 2～ 2 8周龄 )各 14只 ,心脏取血 ,用自动生化分析仪检验血生化及电解质等指标。结果　年老SHR、SHRsp、WKy大鼠血肌酐平均水平较年轻大鼠分别高 8 5 %、9 2 %和 14 2 % (P =0 0 7;P =0 0 7;P =0 0 4 ) ,但血钙、血磷较低。同龄SHR、SHRsp血磷平均水平均较同龄WKy低 2 5 0 % (P =0 12～P <0 0 0 1)。同龄SHR、SHRsp的肌酸磷酸激酶同工酶 (CK MB)及乳酸脱氢酶同工酶 (LDH 1)较WKy升高 1～ 2倍 (P =0 15～P <0 0 0 1)。结论　SHR、SHRsp及Wky三种品系大鼠的血液生化参数多为相似 ,但也有一些差别。

Abstract:目的　制备多种抗泰泽病原体的单克隆抗体 ,用于该菌隐性感染的检测。方法　用含泰泽病原体的大鼠肝匀浆免疫BALB c小鼠 ,采用杂交瘤技术和间接免疫荧光方法筛选抗该菌的单克隆抗体 ;运用免疫电镜、Westernblotting和免疫双向扩散试验确定单克隆抗体的抗原定位和相对分子质量及IgG亚类型。结果　共筛选出 5种单克隆抗体 (M2、M3、M4、M5和M7)。免疫电镜表明 :抗体M2、M4、M5、和M7与泰泽病原体的细胞壁结合 ,而M3为抗菌毛的单克隆抗体 ;Westernblotting结果表明 :抗体M2、M3和M4与相对分子质量约为 6 4× 10 3的抗原有特异性结合 ,M5与M7与相对分子质量约为 4 1× 10 3的抗原特异性结合 ;M2、M5、M7为IgG1 ,M3为IgG2b,M4为IgG2a。对模拟自然感染大鼠考的松激发试验阳性为 2 10 ,而单克隆抗体为诊断抗体的IFA则为 8 10。除M7外 ,4种单抗不与清洁级以上大鼠回盲部细菌发生反应。结论　筛选的杂交瘤细胞能稳定分泌抗体 ,其IFA效价达 1∶10 2 4 ;表明这些抗体具有高效价、较高特异性和敏感性。

Abstract:Objective To study the distribution of cerebrospinal fluid contacting somatostatinergic neurons in the periventricular area of the third cerebral ventricle in rats.Method Immunocytochemical method was used for the observation. Results Some of somatostatin positive neurons were distributed in the ependymal and subependymal layers of the third ventricle.Conclusion There are cerebrospinal fluid contacting somatostatinergic neurons in the periventricular area of the third ventricle in rats.

Abstract:目的　观察通痹片对大鼠佐剂性关节炎 (AA)动物模型的治疗作用及其免疫机制。方法　制做大鼠佐剂关节炎动物模型 ,经通痹片治疗后 ,观察关节指数 ,检测大鼠关节液和血液中IL - 1、TNFα、IL - 6、IL - 8的含量、腹腔巨噬细胞分泌细胞因子 (IL - 1、TNFα、IL - 6、IL - 8)的能力、脾淋巴细胞分泌IL - 6、IL - 8的能力。结果　治疗组关节指数明显低于AA对照组 (P <0 0 1) ,能明显改善AA大鼠的关节炎症状 ;给药组大鼠血清中IL - 1、TNFα、IL- 6和IL - 8水平较AA对照组明显降低 ;腹腔巨噬细胞分泌细胞因子 (IL - 1、TNFα、IL - 6、IL - 8)的能力 ,以及脾淋巴细胞分泌IL - 6、IL - 8的能力显著受到抑制 ;关节液内炎症细胞因子的水平明显下降。结论　通痹片对佐剂性关节炎有良好的治疗作用 ,其抗炎作用的发挥与其免疫抑制反应有关。

Abstract:Objective To question whether No-1886, a synthetic compound, decreases plasma glucose and free fatty acids or improves pathological changes in diabetic Guizhou Minipigs induced by high sucrose and high fat feed. Methods 15 Guizhou Minipigs about 3-4 months were randomly divided into 3 groups of normal control, diabetes, diabetes treatment, which were fed by basal diet,high sucrose and high fat feed or with 1.0% No-1886 respectively.The concentration of plasma glucose and free fatty acids of minipigs,which were fasting the previous night,were analyzed in the 0,2,3,4,5,6,8th month respectively.These minipigs were put to death at the end of 8th month, liver pathologic changes were observed under microscope after the small lumps of liver were fixed with formalin and stained with Oil Red O or liver glycogen (PAS). Results The high plasma glucose and free fatty acids were observed one month late and gradually increased in the diabetes group but not in normal control group(P<0.05).The plasma glucose and free fatty acids of diabetes treatment group were lower than those in diabetes control group. The normal liver structure and abundant glycogen were observed in the treatment group. There were obvious liver fatty degeneration, many fat bubbles, local soluble necrosis, infiltration of a few lymphocytes and little or no glycogen in diabetes group. Conclusion No-1886 improved lipid metabolic disorder and liver fat pathological changes,and decreased plasma glucose or liver glycogenolysis.No-1886 may be developed as an agent to treat glucose metabolic disorder or lipid disorder in diabetes.

Abstract:Objective To observe the effect of beddings mate from different materials on the reproduce of SD rats and the growth of their young rats, and to discuss the feasibility of cornstalk as a new kind of laboratory animal bedding. Methods Some parameters of the reproduce of SD rats and the growth of their offspring were detected and analyzed when they were housed on different laboratory animal beddings made from cornstalk,wood shaving and toilet paper. Results There were no statistical differences among three groups in the number of littermates,the total birth weight and the growth of offspring(P>0.05). But there were significant statistical differences (p<0.05)in ablactation rate (the highest in the cornstalk group,reaching 99.79%)and the interval between two litters (31.41 days in the cornstalk group). Conclusions SD rats could keep high reproductive performance when housed on the bedding made from cornstalr, so cornstalk could be used for a new type bedding material for rats.

Abstract:Objective To establish the data bank of biological parameters of Hubei closed colony Japanese white rabbits for providing biomedical toxicology experiments with relative data of laboratory animal.Methods The rabbit body weight and organ weight were measured. The some of haematological parameters such as RBC, HGB ,MCV, MCH, MCHC, HCT, PLT, MPV, WBC, W-SCR W-LCR were examined. The biochemical parameters of AST,ALT,ALP,BUN,TP,ALB,GLU,T-BIL,Grea,T-CHO were detected. Those were compared with the parameters of common experimental rabbits. Results and Conclusion All the above mentioned parameters were obtained. The biological characters were different among the different species of rabbits, even in same species, and different biological characters due to different regions, environments, breed statuses and nutrition.

Abstract:Objective To further confirm the analgesic effect of external applied Chinese traditional medicine Ai Zheng Zhen Tong San in animal experiments. Methods Glacial acetic acid test in mice and NaCl test in rats were used to make the body twisting then to evaluate the efficacy of the drug. Results When external applied on the belly, Ai Zheng Zhen Tong San restrained the body twisting effect in mice induced by injecting 0.6% glacial acetic acid into abdominal cavity. And it also restrained the body twisting effect induced by injecting 4% NaCl into abdominal cavity in rats. The drug showed good correlation between doses and efficacy. It presented apparent analgesic effects under high and middle doses, while not under low dose. Conclusion Ai Zheng Zhen Tong San is effective when externally applied on the belly for inner ache of the abdominal cavity.

Abstract:Objectiveive To establish a model of white blood cell(WBC) and marrow nucleated cell decreasing in rats. Methods Twenty SD rats were randomly divided into experimental group and control group. In experimental group, cyclophosphamide (30 mg/kg) was injected into rats' abdomen cavity once a day for 5 days,and in control group ,rats were treated with isovolumetric normal saline only. The total of WBC and the number of marrow nucleated cells as well as body weight were monitored. Results In experimental group,the total of WBC in peripheral blood and the number of marrow nucleated cells decreased significantly (P<0.01).The total of WBC was reduced to minimum by the end of the fifth dosing day,and the low level of WBC maintained for 15 days after stopping dosing ,which could provide ample observation time for experimental study. In addition ,rats lost body weight markedly compared with that in control group (p<0.01). Conclusion It was feasible and practical that cyclophosphamide induced the model of WBC and marrow nucleated cell decreasing in rats. Such animal pattern could be used to screen drugs for diminishing chemotherapy toxicity and to develop other therapy methods.

Abstract:Objective To study a BV antibody surveillance model for establishment and maintenance of Macaca mulatta SPF colony. Method The BV antibodies of Macaca mulatta offspring were monitored by BV EIA and BV ELISA after weaning. Results 218 weaned cynomolgus monkeys and 92 weaned rhesus monkeys were selected randomly from the Macaca mulatta offspring in our center. By strictly screening nearly two years, 25 SPF cynomolgus monkeys and 9 SPF rhesus monkeys were obtained,and all the virus test results were confirmed as negative by BioReliance Co. in USA. Conclusion It is suggested that this model can effectively monitor the BV antibody change, and provide a powerful technique support for our center to establish the first Macaca mulatta SPF Colony in our country.

Abstract:Objective To establish the male mouse model with kidney yang deficiency with in reproduction function observed. Method LDH-X of testis alive embryos of female mouse,activity and density of semen and amount of teratospermia were detected in the mouse treated by excess sexual intercourse to establish the male mouse model with kidney yang deficiency,in comparison with spleen deficiency and kidney deficiency by trepidation and hydrocortisone. Results LDH-X of testis, alive embryos of female mouse,activity and density of semen and amount of teratospermia showed statistical difference in group of kidney yang deficiency than other groups (0.01

Abstract:目的　提取嗜肺巴氏杆菌外膜蛋白 (OMP)和脂多糖 (LPS) ,测定其稳定性和抗原性 ,探讨其作为诊断抗原的可能性。方法　选取具有代表性的嗜肺巴氏杆菌 15株及参考株共 16株 ,提取OMP、LPS ,通过SDS -PAGE、Westernblot、ELISA等方法在稳定性、抗原性等方面与全菌抗原 (WC)进行比较。结果　 16株嗜肺巴氏杆菌OMP的SDS-PAGE图谱基本一致 ,不同培养基成分、培养时间和不同体外传代次数的提取物均显示 5条带 ,相对分子质量大约是 (17、2 6、31、37、4 1)× 10 3。其中 5株嗜肺巴氏杆菌的LPS为光滑型和粗糙型LPS的混合物 ,主带相对分子质量在 14 .4× 10 3左右。外膜蛋白和脂寡糖提取物保持了其抗原反应性和免疫原性。OMP和LPS具有种和型的特异性。OMP的 5条条带抗原性存在差异 ,相对分子质量 (2 6、37)× 10 3的蛋白不具有抗原性 ,相对分子质量 17× 10 3的蛋白抗原为受试菌株的共同抗原 ,(31、4 1)× 10 3抗原具有大鼠株和小鼠株的特异性。LPS表现出较强的型的特异性。结论　本研究提取的OMP和LPS活性稳定 ,并保持了抗原性和免疫原性。

Abstract:Objective To improve technique in making heart transplantation model in rats and decrease operative mortality.Method Modified surgical technique was used in donor heart collection, that is, right pulmonary artery instead of main pulmonary artery was anastomosed to inferior vena cava. Perioperative managements were also improved.Results 40 heterotopic heart transplantations were performed. 12 recipients died. Surgical technique, anesthetic accident, hypoxia, anastomotic bleeding may account for operative mortality.Conclusion Modified surgical technique and careful perioperative managements can contribute to success in heart transplantation in rats.

Abstract:Objective To investigate the surgical perioperative management of establishing a rat model of orthotopic liver transplantation.Methods The model was established with modified cuff technique. The donor was perfused through the abdomial aorta with 20 ml cold perfusate. The anastomosis of the suprahepatic vena cava (VC) was sutured end-to-end with 8-Onylon line, and continuity of infrahepatic VC and portal vein(PV) was established by means of cuff method respectively. The bile duct anastomosis was performed by internal stent. Results Three hundred sixty cases of rat orthotopic liver transplantation were performed, and the successful rate of the mode was 91.3%. In non-intervention group, the survival rats were satisfactory, with a 86.5% of 1-week survival and a 80.7% of long-term survival (longer than 3 months).Conclusion Orthotopic liver transplantation in rats can be performed smoothly, the complication reduced largely, and the long-term survival raised under positive preoperative preparation,familiarity with operative procedure, and intensively post-operation nursing and observating.

Abstract:Objective To establish metrorrhagia animal model induced by endometritis in experimental Rhesus monkey. Methods Seven normal menorrhea adult %Rhesus monkeys% were choosed and divided into experimental group and control group at random. Escherichia coli,%staphylococcus aureus% and %peptostreptococcus% were inoculated into uterine cavity of experimental group in the third day of menstruation. Then the cervical secretions were cultured and evaluated in the fifth day of inoculation and the changes of menstrual amount and duration were observed in three continuous menstrual cycle. The sample of endometrium was obtained in late secretory phase of the fourth menstrual cycle for examination under microscope. Endometrium of Control group was not infected, the other procedures were the same as experimental group. Results 1.The evaluation of cervical secretion culture and confirmed that the three kinds of bacteria were inoculants in the experimental group. 2.Compared with control group, experimental group was more excessive in menstrual amount and much longer in duration. 3. Compared with control group, endometrium of experimental group showed the change of chronic inflammation, in which the degeneration of spiral arteriolar wall was more severe and dilatation of lumen more obvious. Conclusion the model comes true by evaluation of the menstrual change and endometrium morphology observation.

Abstract:The macaque was anaesthetized by injecting ketamine hydrochloride in muscle before fixing the head of the monkey in the stereotactic frame. And then, The craniotomy for the monkey could be done. The result showed no complications and deaths due to craniotomy in all these monkeys with operation. The method is a stable anaesthetization to make a model of head injury or to transplant the stem cells under exposure of the parietal lobe, occipital lobe, temporal lobe or the posterior of the frontal lobe.

Abstract:The laboratory animal science have made much more progress for past over ten year. But, as affected by many factors, there are many of shortcomings in animal kinds, animal quantity, environment equipments of experiment and so on. This paper would present suggestions that through strenthening leadship and traning professional people and working out a comprehensive program, the development of laboratory animal science could be sped up.