Abstract:目的　保持自发性高血压大鼠 (SHR)的高血压特性。方法　采取兄妹交配方法 ,观察记录SHR 2年的受孕率及产仔数。分别于 1 2、1 6、2 0周龄测量种鼠的收缩压和心率。结果　第一年度生产 3代 ,平均SHR受孕率及孕鼠平均产仔数分别为 77 5 %与 7 4只 ,SHR受孕率低于同期京都威斯特大鼠 (WKy)受孕率 (90 .0 % ) ,但差异不显著 (P =0 1 2 ) ;SHR平均产仔数高于WKy(6 5只 ) (P =0 0 1 )。第二年度生产 2代 ,平均SHR受孕率及孕鼠平均产仔数分别为 82 7%与 6 9只 ,SHR受孕率及平均产仔数高于同期WKy(5 8 8% ,P =0 0 0 1 ;4 3只 ,P <0 0 0 1 )。第一年度第 1 2、1 6、2 0周龄时雄鼠收缩压平均值为 (1 87 7± 9 0 ) ,(1 97 0± 7 5 )和 (1 99 0± 7 6 )mmHg(1kPa =7 5mmHg) ,第二年度第 1 2、1 6、2 0周龄收缩压平均为 (1 77 5± 1 0 7) ,(1 97 9± 7 2 )和 (1 99 7± 6 8)mmHg。结论　SHR繁育能力近似于WKy;保持了SHR高血压的基本特性

Abstract:目的　了解两种不同环境中饲养的长爪沙鼠寄生虫感染状况。方法　根据 2 0 0 1年中华人民共和国国家标准GB 1 4 92 2 1— 2 0 0 1《实验动物寄生虫学等级及监测》和GB T1 84 4 8 1～ 1 84 4 8 1 0— 2 0 0 1《实验动物寄生虫学检测方法》 ,参考实验动物小鼠、大鼠、豚鼠和地鼠寄生虫等级及检测办法进行检测。结果　饲养于普通环境的 1 5笼 80只长爪沙鼠体表寄生虫感染率为 3 75 % (3 80 ) ,体内寄生虫中卡氏肺孢子虫感染率为 6 2 34% (4 8 77) ,鼠三毛滴虫感染率为 86 2 5 % (6 9 80 ) ;饲养于清洁级环境的 7笼 2 3只长爪沙鼠中 ,卡氏肺孢子虫的感染率为 6 9 5 6 % (1 6 2 3) ,鼠三毛滴虫感染率为 6 9 5 6 % (1 6 2 3)。结论　净化后饲养于普通环境中的长爪沙鼠与净化后饲养于清洁级中的个体相比 ,二者体表寄生虫感染的状况有一定差别。但是 ,卡氏肺孢子虫和鼠三毛滴虫的感染情况似乎无明显差别。

Abstract:Objective To study the effect of glucose change on expression of prepro orexin and its receptors, orexin 1 receptor (OX 1R) and orexin 2 receptor (OX 2R) in rat hypothalamus tissue and pancreatic islets cells. Methods The acute hypoglycemia rat model was induced by subcutaneous injection of insulin. The effect of 2 8 mmol/L and 8 3mmol/L glucose on pancreatic islet cell orexin system was detected in pancreas islet cell culture. The expression of prepro orexin and OXR mRNA was examined by reverse transcription polymerase chain reaction in rat hypothalamus tissue and pancreatic islets cell. Results Expression of orexin mRNA increased about 150% in insulin induced hypoglycemia rat hypothalamus tissue, compared to control group ( P <0 01), whereas expression of OX 1R mRNA decreased up to 30% ( P <0 01). However, expression of OX 2R mRNA did not show significant change in comparison with control group. The expression of prepro orexin mRNA increased up to 100% in rat pancreas islet cell cultured in 1640 medium with 2 8 mmol/L glucose, compared to 8 3mmol/L glucose after 6 h ( P <0 01). But the expression of OX 1R mRNA level was not sensitive to acute glucose fluctuation. Conclusions The orexin and OX 1R are stimulated by falls in plasma glucose and inhibited by signals related to feeding. Moreover, glucose plays a role in modulating the gene expression of prepro orexin in rat pancreatic islet cells.

Abstract:目的　对生物农药白僵菌原药进行急性经口、经皮、吸入毒性试验 ,眼刺激试验和致敏 (皮肤变态反应 )试验。方法　按照《农药登记毒理学试验方法》(GB1 5 6 70— 1 995 )进行操作。结果　该受试物对雄、雌性大鼠急性经口LD50 >5 0 0 0mg kg ;对雄、雌性大鼠急性经皮LD50 (4h) >2 1 5 0mg kg ;对雄、雌性大鼠急性吸入LC50 (2h) >5 0 0 0mg m3;对实验兔急性眼刺激的平均指数为 0 ;对豚鼠致敏 (皮肤变态反应 )试验 ,致敏率为 1 6 7%。结论　该受试物急性经口属微毒性、急性经皮属低毒性、急性吸入属微毒性 ,眼刺激试验为无刺激性 ,致敏 (皮肤变态反应 )试验属Ⅱ级轻度致敏物

Abstract:Objective To evaluate preliminarily safety and effect of Luo Tai injection, and to observe some values of physiology, biochemistry and hemorrheology in rhesus monkey. Methods The animals in two groups were treated daily by 40mg/kg and 24mg/kg in intravenous infusion separately for two period of treatment. The physiological changes were observed every day. Some values of biochemistry, hemorrheology were detected and compared at the early, medium and later stages of treatment. Results There was no effects on physiological function, liver and kidney function in rhesus monkey. The fibrinogen, hematocrit values in test groups were significant lower than those in control group ( P <0 01, P <0 001), and whole blood viscosity, plasma viscosity were lower in the low dose group ( P <0 05). Conclusion Luo Tai injection has obvious function to reduce blood viscosity level in rhesus monkey, and it is safe and effective.

Abstract:Objective To detect the effect of formaldehyde on the activity of superoxide dismutase (SOD) and the concentration of malondialdehyde (MDA) in the lung tissue of the mice after they were made to inhale different amount of formaldehyde. Methods 36 mice were randomly divided into 6 groups, one of which was for control, and the other five were 20,40,60,80 and 100mg/m 3 dose group respectively.The mice were killed after they were given inhalation exposure of formaldehyde in a peaceful environment for 35 days,2 hours per day and then the activity of SOD and the concentration of MDA in the lung were measured. Results The activity of SOD in different dose groups was significantly lower than that of the control group, and the concentration of MDA were remarkably higher ( P <0 01). The activity of SOD and the concentration of MDA had no remarkable differences between the different dose groups( P >0 05), but the former showed an reduced activity, and the latter showed a higher concentration with the dosage up. Conclusion This study suggested that the activity of SOD and the concentration of MDA in the lung tissue of mice could change remarkably after they were given inhalation exposure of some amount of formaldehyde.

Abstract:Objective To study the effect of different gender,age and microbiological background on normal values of blood in Wistar rats. Methods Coulter JT was used for measuring the normal values of blood. Results There were very significant differences between aged male and female SPF Wistar rats in the values of RBC, Hgb, MCV, MCH, PLT, RDW ( P <0 01),and significant differences in the values of WBC,Neut ( P <0 05).By comparing conventional Wistar rats with SPF Wistar rats, the WBC,Neut,Lym,RBC,PDW,MPV had very significant differences( P <0 01),and Hgb,HCT,MCV,PLT had significant differences( P <0 05).For differences between young and aged Wistar rats, Neut,Mono,Hgb,MCH,PDW,MPV were very significant( P <0 01), and WBC,PLT were significant( P <0 05).Conclusion Different gender,age and gender, microbiological background of Wistar rats can affect their normal values of blood.

Abstract:目的　探讨抗原修复技术在脱钙组织冰冻切片免疫组织化学染色中的作用 ,并寻找最佳的抗原修复方法。方法　将“低温微波 硝酸快速脱钙方法”处理的骨及其软组织冰冻切片分为 4组 :Ⅰ对照组 (不处理 )、Ⅱ组(胰蛋白酶 )、Ⅲ组 (微波 枸椽酸 )、Ⅳ组 (微波 TritonX 1 0 0 )。应用LSAB技术检测ANP、5 HT、S 1 0 0、NF、GFAP、Vimen tin、CD31、CD34、FⅧ和VEGF和 1 0种抗体在I～Ⅳ组中的表达 ,图像分析各组阳性细胞的平均吸光度 (A)值 ,结果进行统计处理。结果　Ⅱ～Ⅳ组的吸光度值 (分别为 0 87± 0 4 1、2 .4 3± 0 35、和 2 .4 9± 0 4 3)明显高于I组 (0 4 1±0 37) ;Ⅲ～Ⅳ组明显高于Ⅱ组 ,差异有非常显著性 (P <0 0 1 ) ;Ⅲ、Ⅳ组间差异无显著性 (P >0 0 5 )。结论　用微波 TritonX 1 0 0或微波 枸椽酸缓冲液对脱钙组织冰冻切片进行微波抗原修复是必要的 ,能明显提高免疫组化染色的敏感性。

Abstract:Objective To study the effect of Jinkuishenqiwan(JKW) on the model of kidney-Yang deficiency in rats. Methods Rats were devided into five groups:normal group, model group,therapeutic groups(high,middle,low dose). Except the normal group,the others were made into model of kidney yang deficiency by injecting hydrocortisone. After that, normal and model groups were given equal distilled water , therapeutic groups were given corresponding dose of JKW for twenty succesoive days. In the end, contents of SOD,GSH PX,MDA,testosterone and the organ indexes of every group were determined.Results The contents of SOD,GSH PX,testosterone were obvious higher and the contents of MDA were lower in therapeutic groups than in model group. Meantime,in the therapeutic groups the body weight was higher, the renal indexes were lower than in the model group. And the testis indexes of the high dose group were obvious higher. Conclusion It is indicated that JKW exerts a certain therapeutic effect on the kidney yang deficiency in rats.

Abstract:Objective To establish a preferable model of myocardial infarction(MI) for cellular cardiomyoplasty.Methods MI was made by combined methods of coronary ligation and C shape cryoinjury. Results Small area of MI on left ventricular free wall could be made by above methods successfully.Conclusion This model has many advantages for experimental study on cellular cardiomyoplasty .

Abstract:目的　建立较理想的幽门螺杆菌 (Hp)相关性慢性胃炎大鼠模型。方法　二级雄性Wister大鼠 4 3只 ,随机分为制模组 [H .pylori及 1 甲基 3 硝基 1亚硝基胍 (N methyl N’ nitro N nitrosoguanidine ,MNNG)组 ]1 3只、对照Ⅰ组 (正常对照组 ) 1 0只 ,对照Ⅱ组 (H .pylori感染组 ) 1 0只 ,对照Ⅲ组 (MNNG组 ) 1 0只。对模型组动物 ,采用H .pylori及MNNG的综合方法制模 ,共 1 2周。采用胃黏膜涂片革兰染色和快速尿素酶试验检测H .pylori定植情况。并对胃窦黏膜组织学各项指标进行评定。结果　 (1 )H .pylori感染情况 :正常对照组和MNNG组 :其胃窦黏膜无H .pylori感染 ;H .pylori感染组和制模组分别处死 9只、1 0只大鼠 ,其中 8/9、7/1 0胃窦黏膜有H .pylori定植。 (2 )胃窦黏膜病理组织学变化 :制模组胃窦黏膜变薄 ,固有层腺体中度减少 ,未见腺体化生及不典型增生 ,伴有中度慢性活动性炎症。MNNG组胃窦黏膜变薄 ,固有层腺体中度减少 ,亦无腺体化生及不典型增生。伴轻度慢性炎症 ,但无明显急性活动性炎症。H .pylori感染组仅有中度慢性活动性炎症 ,胃窦黏膜厚度正常。正常对照组胃黏膜基本正常。制模组慢性活动性炎症与H .pylori感染组比较 ,差异无显著意义 ,P >0 0 5。萎缩性病变 [病理积分 (2 2±0 4 2

Abstract:Objective To explore a feasible method and time for establishment of acute myocardial infarction(AMI) in rats. Method Experiments were performed in 38 Wistar rats with respirator. After anesthetized with phenobarbital sodium, a left lateral thoracotomy was performed through 3-4 intercostals region. The left anterior descending coronary artery (LAD) was ligated by 8-0 sutures to shut the blood supply of myocardium controlled by LAD. Acute myocardial infarction (AMI) created by this technique were confirmed by continuous electrocardiographic monitoring, pathologic section and echocardiographic study. Results After ligation of LAD, ECG displayed ST elevated continuously and pathologic Q wave. Pathologic section suggested myofibers chaotically arranged, myofilament ruptured or lysed, nuclei pyknosis and fragmentation, interstitial hyperemia and edema, and infiltration of inflammatory cells. Meanwhile, echocardiographic study showed ejection fraction didn't descend markedly after ligation of LAD. Conclusion AMI could be created by surgical ligation of LAD,and the animal model would be conducive to the basic study on AMI.

Abstract:Objective To explore the effect of thiopentone, ketamine and diazepam on combined anesthesia in rabbits.Method Thiopentone(2 5%,0 6 ml/kg) was slowly injected into 116 experimental New Zealand rabbits to perform anesthesia induction,followed by intraperitoneal injection with mixture(1ml / kg) of ketamine (1 25%) and diazepam(0 125%) preoperatively.If palinesthesia and restlessness appeared, intraperitoneal injection with drugs(mixture, 1ml / kg; thiopentone,0.5 ml / kg) would be applied repeatedly.Results No rabbits died.The combined anesthesia sustained 30-45 minutes each time,and satisfied experimental operation.Conclusion Anesthesia effect can be safely and rapidly achieved by anesthesia induction combined with intraperitoneal injection.Thiopentone and ketamine show the similar anesthesia time, which can be easily controlled. The combined anesthesia is suitable for experimental operations on rabbits, such as on limbs,spine or brain.

Abstract:Objective To meet the requirements of scientific research, the fetus tissues are obtained from 12 mid|pregnant Macaca Mulatta through caesarean birth. Method Caesarean section was made in Macaca Mulatta . Results The differences were found between Macaca Mulatta's caesarean birth and human's. Conclusion The study of caesarean section would be beneficial not only to the experimental research relevant to Macaca Mulatta and to the protection of this species, but also to the reduction of death caused by the section.

Abstract:与一般的软组织不同 ,骨组织石蜡切片在脱水、透明等制片前需要使用脱钙剂除去骨组织中的钙盐才能制备成 4～ 5 μm厚的切片。为了更好地了解脱钙技术对骨组织免疫组织化学染色质量的影响 ,本文根据骨组织及其免疫组织化学染色的特点 ,结合文献对影响骨组织免疫组织化学染色质量的组织的固定、常用的脱钙方法及脱钙液、低温 微波快速脱钙技术及注意事项等方面进展进行了综合分析。

Abstract:Laboratory animal bedding should provide a surface that is hygienic and that fulfils both physical and chemical requirements essential to the health and well being of the animals. It should be chosen in view of its absorbent capacity, its availability and low dust content. Wood appears to be the most suitable raw material for laboratory animal bedding. Of the softwoods, pine and cedar are not usable because of their high resin content of toxic substances for laboratory animal. Scientists demonstrated some changes in microsomal oxidative enzyme activity and other damages in the mouse and rat depending on the bedding. Aspen, birch, fir, and spruce seem to be good sources, as well as corncob and recycled paper. Based on preference testing, the size and the shape might be the major factors in the suitability of bedding particles for laboratory rodent animals. Bedding consisting of large fibers seems to be preferred. Sterilization is recommended. The paper reviews the history and development in criteria of biological evaluation of laboratory animal bedding, with author's opinion about rodent laboratory animal bedding in China.

Abstract:ES cells can renew and maintain pluripotent and undifferentiatial state. Recent years,many researchers pay more attention to the bio characteristics of it, which lay the foundation for its isolation and identification.

Abstract:In this article, applications of chicken embryo in tumor research concerning transplanted tumor models, invasion and metastases, angiogenesis, tumor therapy and viral oncology were reviewed. The chicken embryo is regarded as a suitable material for tumor research.