Abstract:Objective To establish the convenient animal model of herpes simplex virus type-1(HSV-1) latent infection. Method BALB/c mice were divided into 3 groups: intravenous inoculation group,ocular abrasion group and intranasal inoculation group. Six weeks later the trigeminal ganglia(TG) were removed to detect the expression of HSV-1 antigen and the HSV DNA in TG was also detected by PCR to identify the existence of latent infection. Result The detected rate of HSV DNA in TG in intravenous inoculation group was 9/12 and was 13/16 in ocular abrasion group, which were higher than that in intranasal inoculation group(12/20). The expression of HSV antigen in TG in these 3 groups was all negative.The mortality of intravenous inoculation group was 4/15 which was much higher than those of two other groups. Conclusion The methods of intranasal virus dripping, cornea abrasion and intravenous injection can all establish the HSV-1 latent infection model in mice. Compared to two other groups cornea abrasion group shows less severe signs and a quicker recovery rate in acute infection and higher incidence rate of latent infection, so it is the ideal method to establish the HSV-1 latent infection model.

Abstract:Objective To study the LDH and GDH of the swordtail inbred strain RR-B with isoenzyme electrophoresis and analyze genetic purity of strain RR-B. Methods Using polyacrylamide gel electrophoresis, two isozymes(LDH and GDH) of swordtail fish were studied; the LDH of eye and GDH of liver were analysed in both unbreeding and RR-B strain swordtail fish. Results Swordtail fish RR-B strain has same zymogram of LDH and GDH,while the isozyme patterns of swordtail fish are polymorphic. Conclusion The inbred strain of swordtail fish has reached a high degree of genetic purity.

Abstract:目的　用离体蟾蜍肾上腺髓质嗜铬细胞建立刺激 分泌偶联的模型。方法　用胶原酶 (0 5mg ml)消化蟾蜍肾上腺 ,再用牛血清白蛋白 (5mg ml)终止消化 ,获得离体的蟾蜍肾上腺髓质嗜铬细胞。用乙酰胆碱 (2 0 0μmol L)或高K (6 5mmol L)刺激离体嗜铬细胞分泌儿茶酚胺。结果　消化培养后获得了离体的蟾蜍肾上腺髓质嗜铬细胞。ACh(2 0 0 μmol L)能刺激离体细胞分泌CA ,回归方程为Y =- 16 12 16 94X ,r=0 995 1>α0 0 1 =0 95 87;K (6 5mmol L)也能刺激离体细胞分泌CA ,回归方程为Y =12 5 3 7 933X ,r =0 994 2 >α0 0 1 =0 95 87。结论　离体的蟾蜍肾上腺髓质嗜铬细胞是理想的刺激 分泌偶联模型。由ACh和高K 促进的嗜铬细胞CA分泌具Ca2 浓度的依赖性。

Abstract:Objective To determine biological characteristics of Beagle during lactation period. Method Body weight, body length, tail length, body height, belly and chest were measured in Beagle of male and female, each 30, from birth to 62 days. Results With statistic analysis and drawing,the growth in first 30 days was almost the same, afterwards growth rate of male was higher than female. Growth indices of female and male suggested their simultaneous development.

Abstract:Objective To observe the effect of autotransfusion in postoperative miniature pig.Methods 49 miniature pigs on operation were randomly divided into two groups, that is, there were 22 miniature pigs in normal control group to which nothing was transfused. The others were in the autotransfusion group, which was autotransfused in 3 hours. Results The survival rate of autotransfusion group was 81.5%, and survival rate of the control group was 54.4%, showing very significant difference between them(p<0.01).Conclusion It indicates that autotransfusion may be a useful way of increasing the survival rate in postoperative miniature pigs.

Abstract:Objective To explore vitrified method and cryopreservative solution of mouse oocytes. Method The oocytes of ICR and C 57 BL/6J mice were vitrified with EFS40(high concentration of sodium) and DEFS40(low concentration of sodium),thawed with 0.5mol/L sugar solution.Before fertilization in vitro, zona-pellucieda of C 57 BL/6J mice thawed oocytes were dissected. Results Considering the vitrified effect, DEFS40 is better than EFS40. The oocyte survival rate of ICR mice reaches 75.2% after thawed. The fertilization rate reaches 24.6% and the difference is not remarkable compared to the control.And the oocyte survival rate of C 57 BL/6J mice reaches 87.1% after thawed. The oocyte fertilized rate which zona-pellucieda were dissected reaches 46.0% and the difference is not remarkable compared to the control.Conclusion The vitrified effect of mice oocyte is influenced by the concentration of sodium.The perfect effect could be obtained when the oocytes were vitrified with DEFS40.

Abstract:Objective To compare the permeation condition of ligustrazine hydrochloride for skin of SPF laboratory animals BALB/c nude mice,KM mice and SD rats in order to give evidence of fit skin of SPF animals in the osmosis experiment in vitro. Methods An experiment installation of extrinsic permeation skin of the two room diffuse pool was used for the skin to detect the osmosis coefficient and the effect of store depot of ligustrazane hydrochloride in defferent kinds of animals. Results There was a remar kable difference in the absorbent effect of permeation skin for ligustrazine hydrochloride between back and abdomen in the three kinds of animals, among them osmosis coefficient was the highest in back and abdomen of the nude mice. The back skin absorbent effect of permeation skin was better than the abdomen skin in same kind of animals. Deposit function of BALB/c nude mice skin was better than the skin of SD rats. Deposit function of back skin was better than the abdomen in every animal. Conclusion The back skin of nude mice is more suitable for the experiment of extrinsic permeation in usual SPF laboratory animals.

Abstract:Objective To elucidate the role of thyroid hormones during the embryo and juvenile periods in mammal, and to observe the effects of thyroid hormones on the cerebrum development. Method During mid pregnancy of the female sheep with twins, one fetus of the twins was thyroidectomized. The female sheep with twins were kept pregnancy to birth after the operation. The pyramidal cells in the cerebral visual cortex of the twins were with Golgi staining for observation. Result The number of spinous process, the growing cones and basal dendrites of the thyroidectomized sheep were different from the normal group. Conclusion The deficiency of thyroid hormones can influence the development of pyramidal cells in the cerebrum.

Abstract:Objective To investigate effect of Ginsenoside-Rg 1 on the brain in adult rat following local ischemia. Methods The model of cerebral infarction in the territory of middle cerebral artery in rat was prepared, Ginsenoside-Rg 1 was injected to abdominal cavity with different doses, and the neurological function deficits were examined and analysed. Results In the 1st day group, no difference was found (P>0.05)in comparing every dose (50 mg/kg, 100 mg/kg, 200 mg/kg)with positive control group and negative control group. In the 3rd day group, there was no difference(P>0.05) compared with positive group, but a marked difference(P<0.05)was showed in the 100 mg/kg dose group compared with negative control group. In the 7th day group, neurological function analysis revealed great difference (P<0.05) in comparison with both pasitive group and negative group. Conclusion Ginsenoside-Rg 1 could produce effects of treatment and protection to a certain extent when it was used for local ischemia, with time-effect relationship, but with no dose effect relationship. The treatment duration should be longer, say, seven days.

Abstract:Objective To build up a steady and credible animal model with verging on clinic pathological state of obstructive sleep apnea (OSA). Methods Ten China swine were divided into two groups (A and B, 5 each). Group A was treated by injecting polyacrylamide hydrogel from exterior. Group B was treated by injecting polyacrylamide hydrogel from interior. EEG, oro-nasal airflow, snoring, chest respiration, abdomen respiration and oxygen-saturation were recorded by PSG after operation. Examination was repeated one week later. CT was done two weeks later. Animals were sacrificed three month later and prepared for examination by light microscopy. Results All animals showed apnea or hypopnea and decreased in SaO 2 after operation. There was no difference between groups. Apnea duration and apnea index in all animals were increased significantly and SaO 2 decreased significantly one week later. The changes in animals of group B were more obvious. As for result of CT examination, there was stenosis in some degree at faucial cavity in animals of group A and obvious stenosis at faucial cavity in animals of group B. Under light microscopy, connective tissue pellicle comprising elastic and collagen could be seen at the exterior of polyacrylamide hydrogel. Conclusions This chronic animal model of OSA in China swine is steady and credible. It verges on clinic pathological state and can be reduplicated. It accords with medical ethnics and has a very slight trauma to the animal. The model can be applied to investigate pathogenesis, pathophysiology and therapeutics of sleep apnea syndrome(SAS) extensively.

Abstract:目的建立简便易行的单纯疱疹病毒Ⅰ型(HSV—1)潜伏感染动物模型。方法BALB／c小鼠60只随机分为3组，分别为鼻黏膜病毒滴入组、尾静脉接种组和角膜划痕接种组，进行单纯疱疹病毒Ⅰ型接种。6周后行小鼠三叉神经节病毒抗原检测和病毒DNA片段检测，以确认潜伏感染的存在。结果尾静脉接种组小鼠三叉神经节病毒DNA片段的检出率为9／12，角膜划痕组小鼠三叉神经节病毒DNA片段的检出率为13／16，均高于鼻黏膜接种12／20；小鼠三叉神经节病毒抗原表达均为阴性；尾静脉接种组死亡率4／15，高于其他两组。结论鼻黏膜病毒滴入、尾静脉注射和角膜划痕接种均可建立小鼠HSV—1潜伏感染模型，以角膜划痕法引起疱疹病毒急性感染症状轻恢复快，潜伏感染发生率高，为最理想的建立HSV—1潜伏感染的方法。

Abstract:Objective To make rabbit atherosclerosis model and to see its stability after stop of feeding high fat forage. Methods Three groups were fed with different amount of cholesterol, lard and yolk respectively. When atherosclerosis was taken shape in aorta, the feed of high fat forage discontinued. Results Over 11 weeks of feeding rabbit endaortic membrane was of full spots of fat deposit, forming atherosclerosis, and 15 weeks afterwith drawal of feeding atherosclerosis stayed. Conclusion Rabbit atherosclerosis model was stablely established in this way.

Abstract:目的　探讨造影剂误入动物蛛网膜下腔后导致脑栓塞形成的抢救和预防。方法　参照人类脑水肿治疗方案和剂量 ,采用连续缓慢注入 2 0 %甘露醇和 10 %葡萄糖酸钙于比格犬前肢皮下静脉内 ,直至该犬大量排尿及身体抽搐基本停止的抢救措施。同时改用小剂量、低粘度碘造影剂 ,并抬高动物头部的方法来预防脑栓塞形成。结果　采用抗脑水肿治疗效果明显而通过肌内注射冬眠灵和安定的抗惊厥处理是无效的。造影剂所致动物脑栓塞的形成能通过上述方法有效地预防。结论　本文所述脑栓塞形成的预防和抢救是有效和成功的 ,它应同样适用于临床同类病人的救治。

Abstract:Objective To establish a sensitive and specific molecular biological method for detecting Staphylococcus aureus (S. aureus, SA) in laboratory animals. Methods A DNA fragment of 676 bps of the nuc gene was applified from SA genome DNA by PCR and labeled with biotin as the probe. The purified genomic DNAs of SA1800, SAm0109 and Staphylococcus epidermidis, Streptococcus pyogenes, Escherichia coli were blotted onto the Hybond N nylon membrane, fixed using UV irradiation, hybridized under high stringency conditions, and detected using the Enhanced Chemiluminesence kit (ECL). Results The probe reacted positively with the SA genomic DNAs, but not with that of the non-SA with a sensitivity of I pg. 322 clinical samples taken randomly from specific pathogen free (SPF) mice were submitted to detection by the dot blotting and previously established PCR and culture with a positive detection rate of 3.1% (10/322) and an agreement of 100 %. Conclusion The dot-hybridization assay was a new rapid, sensitive and specific method and could be applied to detect SA in laboratory animals.

Abstract:目的探讨Beagle犬不同生长时期血液学指标的动态变化。方法采集专业繁殖场50只Beagle犬桡静脉血样，分析测定白细胞(WBC)总数、红细胞(RBC)总数、血红蛋白(Hb)、血小板(PLT)数、中性粒细胞(N)百分率、淋巴细胞(L)百分率等血液学指标的正常参考值及其在不同生长时期的动态变化。结果Beagle犬RBC、Hb和PLT在种犬为最高，幼犬最低；WBC及其分类变化不明显，其中L与N比值为1：2．1，雄性幼犬L值偏低。结论Beagle犬血液学指标在不同生长时期存在一定的变化规律。

Abstract:Through the gene expression and overexpression, transgenic animal models were established. This paper introduced some transgenic animal models and their characters, concerning with lipometabolism genes such as scavenger receptor gene SR-A, SR-B1 and SR-CD, apolipoprotein genes ApoE, ApoA, ApoB and ApoC, enzyme and transit protein genes like CETP and LPL.

Abstract:The Eimeria spp. parasitized in laboratory small animals(rats, mice, hamsters, guinea-pigs and rabbits) are numerous in number and high in frequency.And all the small laboratory animals should be free from all those Eimeria spp. Among Eimeria, only those parasitized in rabbits have been studied systematically, the others are still unknown to us. The following are our opinions based on the information we have. (1)Based on the host specificity of Eimeria, more than 30 spp. of Eimeria are divided into 5 animal groups in order to be easier in species identification. (2)The classification of Eimeria nowadays is based on the morphology of oocyst. It's difficult to distinguish when there are many kinds of Eimeria due to a variety of maturation degree. It's important to point out that both new and old samples are needed. The morphological regularity found by contrast is useful in species identification. (3)The morphology of oocyst is the main point of classification. The creditability of identification worud be increased by the combination judgement based on observing the whole process of Eimeria life-cycle, such as schizogony and gametogony reproduction in the intestinal cells, the needed time of the formation of occyst and the histological and pathological changes.