Abstract:Objective To explore the feasibility of gene therapy for human tumors by direct injection of recombinant plasmids. Methods Mouse tumor model was established by transferring human ovarian carcinoma (SKOV 3) tissues or separated cells subcutaneously into the oxter of nude mice, with a successful rate of 100%. The coding region for human endostatin (hES) fused with the signal peptide sequence of human growth hormone was amplified by PCR from a commercial recombinant plasmid and the correctness of the hybrid gene was confirmed by sequence analysis following subcloning into pGEM-T vector. The hybrid gene was subcloned into a eukaryotic expression vector pcDNA3 and its expression in COS-1 cells was demonstrated by immunofluroscence. Results Significant inhibition of the tumor growth was observed in 6/6 mice after muscular injection of nude mice with established tumor with pcDNA-ES. When the injection was done before tumor transplantation, the tumor could not establish itself in five out of the eight mice, while established in all of the mice (4/4) without plasmid injection. Conclusion These data indicate that the {established} animal model and gene therapy strategy might be useful for further studies on treating human tumors.

Abstract:Objective To establish a mouse aplastic anemia model by using cyclophosphamide and methylbenzene, and research its pathomorphology. Method Cyclophosphamide 50.0mg/kg was injected each other day, four times,with inhalation of toluene (30mg/L, 2h/d for 8 days) to reproduce aplastic anemia model. Control group was injected with equivalent saline subcutaneously without narcotics. Result The peripheral hemogram of mice in the model groups showed that there was a remarkable decrease in three types of cells (p<0.01) and in bone marrow hyperplasia. And there was a distinct increase in lipocytes. The spleen was atrophied, and its lymphnodule became small or disappeared. The microcirculation of ear was obstructed. All indices conformed to aplastic anemia. After 35 days, the pathological changes still existed. The changes were similar to human aplastic anemia, while there wasn't such changes in the control group. Conclusion The method to reproduce aplastic anemia model was simple, and had a high success rate. It could be used to screen relative drugs.

Abstract:目的　进行可见光照射所致大鼠视网膜光性损伤后的行为测试 ,探讨动物视功能与行为的关系。方法　 2 1只SD大鼠在 (95 0± 5 0 )lx绿色光损伤仪光照 3、12、2 4h ,实验前及实验后暗适应 2 4h ,光损伤前 2d及光损伤后 2d测定大鼠的头部视动跟踪反应、前置刺激反应。光损伤后 72h摘除眼球 ,冰冻切片后行HE、Rhodopsin免疫荧光染色 ,光镜观察。实验对照组为 7只双眼球摘除术后的大鼠。结果　随着光照时间的延长 ,动物的头部视动跟踪反应逐渐丧失 ,对照组反应最弱 ,前置刺激反应逐渐增强 ,对照组反应最强。结论　不同程度的视细胞损伤后的动物行为是不同的 ,随着损伤程度的加重 ,动物的行为呈现与之相适应的变化

Abstract:Objective To explore the oxidative inducibility of oh 8Gua glycosylase in mammalian tissues. Methods Treated with potassium bromate (KBrO-3), the activity changes of this enzyme in the kidney and the liver of rats were measured and compared by HPLC/ED. Results With a single dose of KBrO-3 (80 mg/kg,ip), the activity in the kidney was found to increase significantly at 3h. At 6h, activity peaked, showing a 6-fold increase over that at zero time. Thereafter, it decreased and returned to its zero time level at 12h. With increasing doses of KBrO-3 (up to 160 mg/kg,ip),activity increased linearly with increased dosage, and over 40mg/kg, the activity increased to a level significantly higher than that in the control. But no significant changes were observed in the liver on the same conditions. Conclusion oh 8Gua glycosylase can be induced oxidatively in mammalian tissues and it implies that the repair of oh 8Gua residues in DNA is a process important for the survival of organisms in an aerobic environment.

Abstract:目的探讨17β-雌二醇(E2)对斑马鱼初期胚胎的毒性作用。方法用1．0、2．5、50、7．5、10、25、50μmol／L的E给受精后3h的斑马鱼胚染毒，进行形态学观察及原位杂交。结果胚胎在受精后48h，5．0、7．5、10、25、50μmol／L试验组的死亡率分别是0％、36％、6．4％、100％、100％。在7．5、10μmol／L染毒组出现脊柱弯曲、心囊水肿、血流停滞等畸形。使用MyoD探针．观察到体节数的减少及MyoD基因表达明显减弱。结论E2有致死、引起体节生成障碍，致使脊柱弯曲等致畸形作用。而且体节生成障碍可能是由于E2对MyoD基因的影响所致。

Abstract:目的确定Vc-獭免染色体核型与G-带核型。方法外周血淋巴细胞培养、常规染色体制片和染色体G-分带技术，对Vc-Ⅰ系、Vc-Ⅱ系獭免的染色体组型、G-带核型进行了研究。结果与结论Vc-Ⅰ系、Vc-Ⅱ系獭兔二倍体细胞染色体数为44，即2n=44，21对常染色体，1对性染色体(X、Y)。常染色体分为4组：A组，1～8号，为中着丝点染色体；B组，9～11号，为近中着丝点染色体；C组，12～17号，为近端着丝点染色体；D组，18～21号，为端着丝点染色体。X为近中着丝点染色体，Y为端着丝点染色体。Vc-獭兔的公兔染色体组型为44，XY。G-带核型表明：Vc-Ⅰ系、Ⅱ系免的染色体G-带型基本一致。

Abstract:Objective To investigate the effects of %Yangshenguo Xiaoke% Capsule in different doses on the diabetic rabbits by analyzing the blood glucose, body weight, blood biochemical index, blood routine index, hemorrheology and the concentration of insulin. Methods Rendered diabetes due to alloxan monohydrate, the rabbits were administered orally %Yangshenguo Xiaoke% Capsule. in three doses(0.40 g/kg,0.20 g/kg,0.10 g/kg) once a day for four weeks. Then the rabbits' blood was collected and the blood biochemical indices and others were detected. Results The high and middle doses of the drug reduced the blood glucose remarkably compared with control (P<0.05) and could improve the hemorrheology index. Its hypoglycemic effect was similar to %Xiaoke% bolus. Conclusion %Yangshenguo Xiaoke% Capsule has the hypoglycemic effect on diabetic rabbits.

Abstract:Objective To develop an experimental animal model of lung impact injury under the closure of glottis. Methods 108 rabbits were impacted in the right chest with various driving pressures and compression percentage resulting in closed thoracic trauma. Results Different impact conditions caused different degrees of thoracic trauma. The severity of lung injury was positively related with the driving pressure and compression percentage. AIS values of the glottis closure group were significantly higher than those of the open group (P<0.05-0.01). Conclusion The established model is relatively simple, easily controllable and highly repeatable, which could be used as a feasible model for the study of thoracic trauma.

Abstract:Objective To study the effect of low-level ozone on reproductive physiology of mice. Methods Low-level ozone was created by 15W ultraviolet light. The mice in experimental group and control group were fed in low-level ozone environment or normal environment respectively for 7 weeks. The weight variations of both groups were observed, and estrous cycle of female mice was determined by exfoliative cytoscopy of vagina.Then the sperm abnormality test and the micronucleus test of early spermatids were performed in both groups;serum samples were taken for sex hormone determination by radioimmunoassay kit;specimens of spermary and ovary were taken for histopathological analysis. Results No significant difference was found in the weight variation of female mice,but male mice of experimental group were lighter than those of control group;the difference of the estrous cycle of female mice was not significant;no significant difference was observed in sperm abnormality,micronucleus of early spermatids and serum sex hormone level;no histopathological abnormality was observed in both groups. Conclusion Low-level ozone created by ultraviolet light in our research has no effect on reproductive physiology of mice.

Abstract:目的　建立合适的小鼠卵母细胞孤雌激活方法。方法　取不同卵龄小鼠卵母细胞 ,运用不同浓度的氯化锶和不同强度的电脉冲对其进行活化 ,观察小鼠卵母细胞激活率和体外发育状况。结果　① 14、16和 18h卵龄组卵母细胞经 10mmol LSrCl2 处理后 ,三组的激活率随卵龄的增长而提高 ,分别为 2 1 1%、4 1 8%和 73 7% ,组间差异有统计学意义 (P <0 0 5 ) ;卵母细胞离体后在激活前体外培养 3h也能提高其激活率 ,但当卵龄达到一定时 (18h) ,体外培养激活率不再提高。② 5 0、10 0、15 0mmol L三种浓度的SrCl2 均能有效地激活小鼠卵母细胞 ,激活率分别为 5 8 5 %、6 8 95和 70 9% ,与对照组和 1 6mmol L组相比差异有统计学意义 (P <0 0 5 )。③卵母细胞的激活率随处理时间的延长而提高 ,30、6 0、12 0、2 4 0minSrCl2 处理时间的激活率分别为 4 4 9%、5 6 1%、6 8 9%、77 0 % ,相隔两组之间差异有统计学意义 (P <0 0 5 )。④ 1 5kV cm ,16 0 μs和 1 0kV cm ,32 0 μs脉冲刺激下的卵母细胞激活率显著高于 1 8kv cm ,90 μs脉冲刺激组 (5 8 5 %vs2 7 1%、6 9 1%vs2 7 1% ,P <0 0 5 ) ,前两组之间差异无统计学意义 (P >0 0 5 )。结论　小鼠卵母细胞孤雌激活率与卵龄和激活方案有关 ;氯化锶浓度、作用时间和

Abstract:Objective Southern blot hybridization with DIG labeling probe was used to identify whether transgenic mice of human Cathepsin-K gene were positive. Methods Transgenic mice and the intercrossed progeny between the founder mice of human Cathepsin-K gene and wild ICR were detected by Southern blot hybridization with DIG labeling probe. Results 25 transgenic mice were obtained through microinjection, one was detected to be positive by Southern blot hybridization. The positive rate is 4%. Positive F1 generation via PCR was all positive by Southern blot. Conclusion The foreign gene (human Cathepsin K gene) has already integrated into mice chromosome and can inherit to the F1 generation.

Abstract:目的　探讨改进的小鼠腹部异位心脏移植技术的应用效果。方法　同系BALB c小鼠 80只 ,均为雄性 ,体重 2 3～ 2 7g。苯巴比妥纳 (70mg kg)行腹腔内注射麻醉。心脏移植 :在受体腹主动脉及下腔静脉游离后 ,用一弧形小动脉钳将上述血管一并钳夹阻断血流。在 2 5倍显微镜下用 11- 0的尼龙线将供心主动脉与受体腹主动脉 ,肺动脉与下腔静脉作端侧连续吻合。经阴茎背静脉注入林格氏液 0 3ml,然后松开阻断钳恢复血流。结果　成功施行心脏移植术 4 0例。受体手术时间为 (6 1 8± 6 5 )min ,血管阻断时间为 (2 8 6± 3 4 )min ,供体心脏缺血(36 2± 3 7)min。 7d成功率为 92 % (37 4 0 ) ,30d为 90 % (36 4 0 ) ,10 0d为 80 % (32 4 0 )。结论　改进的小鼠腹部异位心脏移植技术是一种简便、成功率高的方法。

Abstract:小鼠作为人类理想的疾病生物模型，不仅同人类具有生理相似性，而且为建立人类疾病模型提供了大量的遗传资源.“人类基因组和小鼠基因组计划”完成后，转基因动物、基因剔除和基因剔入、大规模突变、特异位点检测系统等一些新技术的出现，提高了克隆新易感基因和建立人类疾病小鼠模型的能力、本文对一些与小鼠疾病模发展有关的最新技术进行了总结。

Abstract:The paper introduced the species of aquatic animals and their laboratory research, application and progress at home and abroad in toxicology, pharmacology, environmental monitoring, embryology, genetics, physiology and chemophysiology.

Abstract:Along with the development of medical domain, many achievements on human organ transplantation have been attained and organ transplantation has been routinely applied in clinical practice. But because of infection, reject reaction and surgical techniques,small intestine transplantation has never extensively carried out in clinic. Just for this point, it is important and necessary to set up the reliable animal model. This article makes a review on surgical techniques and characteristic of small intestine transplantation in pigs. It may provide the significant basis for small intestine transplantation in clinic.

Abstract:Three kinds of laboratory model mice in human chronic myelogenous leukemia are available, including xenotransplantation of CML cells into immunodeficient mice, bcr/abl retroviral bone marrow transduction and transplantation,bcr/abl transgenic mice. Recent studies in these different model systems have yielded important advances in our knowledge of the pathogenesis of human chronic myelogenous leukemia.

Abstract:Diabetes mellitus is a common genetic glucose metabolic and endocrinal disturbance caused by insulin deficiency absolutely or relatively. In the last half century, diabetes has the increasing rates of morbidity and mortality, and has become the third common, frequently occurring and chronic noninfectious disease after cardiovascular disease and cancer. Along with the research and production of diabetes drugs, the animal models are well developed.