Abstract:Objective The experiments were carried out to develop an improved in vitro fertilization (IVF) system for Kunmimg mice by comparing the effect of fertilization media and treatment methods on IVF of mature oocytes and the effect of three culture media for early embryo development. Methods Sperm was prepared by sqeezing cauda epididymidis from male mouse and mixed with mature oocyte from superovulated female mouse in T6 or FM media, and mature oocytes were treated with hyaluronidase for removing cumulus cells. The control was not treated before fertilization. The IVF embryos were cultured in M16, modified CZB media (mCZB) or modified M16 media (mM16) media. Results The fertilization rate in FM media was significantly higher than that in T6 media (92 3% vs. 64 7%). The fertilization rate of treatment method to mature oocyte without hyaluronidase before fertilization was higher than that of hyaluronidase treatment method (64 7% vs. 59 2%), but no significant difference was found. The developmental rate of morulae and blastocysts in M16 media (55 3% and 35 6%) were significantly higher than that in mCZB media (17 3% and 14 7%). Conclusion It is indicated that FM medium is more appropriate for in vitro fertilization of oocytes, and it is not necessary to remove the cumulus cells of mature oocyte by hyaluronidase. MM16 media is the most appro priate for in vitro culture of early embryos of Kunming mice, and hypotaurine can efficiently overcome embryo development retardation after 2 cell stage. reofearlyembryos

Abstract:目的探讨多氯联苯(polychlorinatedbiphenyl,PCB)对大鼠肾脏结构及功能的影响。方法用Wistar雄性大鼠40只随机分为4组,Ⅰ组饲喂正常饲料,Ⅱ组饲喂含10-8mol LPCB饲料,Ⅲ组饲喂含10-7mol LPCB饲料,Ⅳ组饲喂含10-6mol LPCB饲料,饲喂3个月后建立慢性PCB毒性动物模型,用光镜、免疫组化技术研究PCB对大鼠肾脏结构及功能的影响。结果PCB对大鼠肾脏结构的损伤同PCB剂量呈正相关,高剂量PCB组肾脏组织结构受损严重;PCB能诱导细胞凋亡抑制因子(bcl2)和转化生长因子β(transforminggrowthfactorβTGFβ)表达,两者的表达强度同PCB浓度呈正相关;高浓度组bcl2和TGFβ1的阳性率明显高于对照组和其他组(P<0.05);结论PCB对大鼠肾脏组织结构及功能产生明显的损伤作用。

Abstract:Objective To investigate the effects of lentinan (LNT) on lipid peroxidation and NO level in brain tissues of diabetic rats. Methods\ Twenty nine male Sprague Dawley rats were randomly divided into three groups: normal control group(n=10), diabetic group(n=10) and LNT treatment group(n=9). The morphological changes of brain tissues were examined by light and electron microscopy. The activity of superoxide dismutase(SOD), nitric oxide synthase(NOS) and content of malondialdehyde (MDA), nitric oxide (NO) in the brain tissues were assayed by spectophotometer, respectivly. Results Cerebral edema, encephalomalacia,demyelination in the white matter were observed by histopathology. Swelling and criste loss of mitochondria in neurons and neuroglial cells, demyelination of nerve fibers and vascular endothelial cell damages were seen by electron microscopy. The activities of SOD in diabetic brain tissues was decreased; but the activities of NOS and content of NO increased in comparison with those of control group. After treatment with LNT, the activities of SOD were increased and NOS activity, content of NO and MDA were decreased in comparison with those in diabetic rats. Conclusion LNT possesses effects on prevention of brain tissue damages in diabetic rats by enhancement of anti oxidation activities and reduction of NO level.

Abstract:Objective In order to evaluate the value of open pulled straw(OPS) method on cryopreservation of embryos of different genotypes of HBV transgenic mice, and to provide a basis for establishment of transgenic embryos bank. Methods Ethylene glygol (EG) was used as the cryoprotectant for cryopreservation of morula and blastocyst embryos of P 21 / , P 21 HBsAg/HBsAg and P 21 HBx/HBx . Result The average numbers of embryo production of P 21 / , P 21 HBsAg/HBsAg and P 21 HBx/HBx were 27 89, 15 56 and 9 14, respectively, after superovulatory treatment. The recovery rates of P 21 / , P 21 HBsAg/HBsAg and P 21 HBx/HBx were 88 7%, 81 1% and 80 3%,respectively. The development rates after thawing were 82 6%, 79 4% and 56 6% in vitro, respectively. The results indicated that the numbers of superovulation of P 21 HBsAg/HBsAg and P 21 HBx/HBx were lower than those of P 21 / . The recovery rates were not significantly different in the three genotypes mice. The survival rate after thawing of P 21 HBx/HBx was lower than that of P 21 / and P 21 HBsAg/HBsAg . Conclusion The genotype of mice affect the results of superovulation number, recovery rate and survival of embryos. The OPS method is feasible and useful in cryopreservation of transgenic mouse embryos.

Abstract:Objective To study the pathological and MR image characteristics of congenital hydrocephalus in Wistar rats. Methods Six Wistar rats with congenital hydrocephalus were found among 9 rats in a litter. The rat heads at age of 7, 14 and 21 days, respectively, were made MR imaging, cut and put into Bouin fixative solution and cut into continuous coronary sections. The viscera organs were also routinely fixed in formalin and prepared for histopathological examination. Results One day old neonates could usually be detected as being hydrocephalic revealing a slightly doming of their heads. Severe hydrocephalus was depicted by MR images. The cerebrospinal fluid was of slightly yellowish color, transparent, with no peculiar smell. No inflammatory cells were found in the cytological smears. The viscera organs did not show obvious abnormalities. The skull was weakly developed with poor ossification. The brain showed a thin cortex, with dilatation of lateral ventricles and partial adhesion, stenosis or obstruction of the cerebral aquaeductus. Some clumps of exfoliated cells were present in the third ventricle. No alterations were observed in the choroid plexuses. Conclusion Our findings in this study indicate that it is a congenital disease. The hydrocephalus is caused by stenosis and obstruction of aquaeductus, showing typical characteristic pathological and MRI alterations. ebrainshowedath ductus.Someclum Conclusion Our

Abstract:目的探讨ATⅡ含量和ACE活性是否与动物年龄的增长存在着相关性及牛磺酸(Taurine)对高血压的预防作用机理。方法对不同周龄大鼠和补充不同水平牛磺酸和β丙氨酸(牛磺酸转运抑制剂)大鼠血液中血管紧张素Ⅱ(angiotensinⅡ,ATⅡ)含量和血管紧张素转换酶(angiotensinconvertingenzyme,ACE)活性进行比较。结果随着大鼠年龄的增长血液中ATⅡ含量随之增高(p<0.05),ACE活性也随之增加(p<0.05),补充牛磺酸后使血液中ATⅡ含量、ACE活性降低(p<0.05)。结论给动物补充牛磺酸对高血压的产生具有预防作用。

Abstract:目的探讨2,3,7,8四氯苯二英(TCDD)对NIH小鼠胚胎发育过程的影响。方法在NIH小鼠妊娠早期的1～8d,胚泡着床前期的1～3d和着床后期的4～8d,经口腔灌服0、2、50和100ng(kg·d)剂量的TCDD,在小鼠妊娠第9天和第18天时观察子宫内的胚胎发育形态。结果50、100ng(kg·d)剂量在妊娠早期1～8d染毒,使多数妊娠小鼠在第9天观察时子宫内胚胎全部丢失;在妊娠1～3d和4～8d染毒,使部分妊娠小鼠胚胎丢失,另外有部分胚胎吸收或发育阻滞。同时发现早期成活胚胎在发育过程中继续死亡,妊娠第18天时,胎鼠出生前成活率下降。结论小鼠妊娠早期染毒,引起小鼠妊娠早期胚胎丢失,着床后胚胎发育异常,出现阻滞和死亡。

Abstract:Objective To investigate the effect of Cortex Eucommiae on the fractures healing process. Methods Tibia fracture models were created in 45 rabbits. The animals were divided into experiment (n=23) and control groups (n=22). The experimental rabbits were intragastrically administered with Cortex Eucommiae and the control animals received the same amount of physiological saline. The fracture ends were taken for light microscopic examination on days 7, 17, 27, 37 and 47 of the experiment and the histopathological data were semi quantitatively analyzed. Results The light microscopic observation demonstrated that the repairing responses occurred earlier in the experimental group than that in control group. Conclusion The concentrated extracts of Cortex Eucommiae taken orally accelerates the healing process of fracture in rabbits.

Abstract:Objective To investigate the effects of Xiaoyao nose dripping liquid on the behavior and symptoms of experimental rat migraine model. Methods Fifty Wistar rats were randomly divided into 5 groups, each of 10 rats: normal control, model group, taijitongtiankoufuye liquid given orally group, Xiaoyao nose dripping liquid given orally group and Xiaoyao nose dripping liquid dripped into nose group. The experimental migraine model rats were duplicated by modified method reported by Tassorelli. The created rat models were given corresponding drugs and their changes of behaviors and symptoms were continuously observed at various time intervals. Result The symptom amelioration by Xiaoyao nose dripping liquid dripped into nose group was significantly better than Xiaoyao nose dripping liquid and taijitongtiankoufuye taken orally groups. Conclusion Xiaoyao nose dripping liquid may significantly improve the symptoms of migraine.

Abstract:目的通过动物实验研究碘过量对大鼠生殖力及仔鼠记忆力的影响。方法将雌性大鼠随机分3组即高碘组、适碘组、缺碘组,每组20只,用缺碘饲料和不同浓度的碘水,复制动物模型。将雄性大鼠与造模成功的雌性大鼠按1∶2的比例合笼交配,观察生育状况及4周龄仔鼠学习记忆力。结果3组大鼠受孕率分别为适碘组85%大于高碘组65%大于缺碘组55%,每孕鼠产仔数量分别为适碘组(10.2±2.5)大于高碘组(7.4±1.5)(p<0.01),适碘组大于缺碘组(6.5±1.5)(p<0.01)。跳台实验中高碘组、缺碘组仔鼠错误次数明显多于适碘组,跳台潜伏期缺碘组和高碘组仔鼠明显低于适碘组仔鼠。结论碘过量会降低雌鼠的生殖力及仔鼠学习记忆力。

Abstract:Objective To establish animal models of intestinal flora regulation and intestinal flora imbalance prevention. Methods 1 The animal model of intestinal flora regulation:40 mice used in this experiment were randomly divided into 4 groups, each of 10 mice. Antibiotics were used for 3 days to create intestinal flora imbalance in mice. Then the mice of control group received saline and the mice in other 3 experimental groups received microecosystem regulator, fructose and traditional Chinese medicine, respectively, for 5 days for intestinal floras regulation. Feces samples of the mice were taken by aseptic manipulation and cultured for assessment of amount of enterobacteriaceae, bifidobacterium and lactobacillus floras growing in the intestine. 2 The model of intestinal flora imbalance prevention: 40 mice were randomly divided into 4 groups, each containing 10 mice. Along with the antibiotics, the mice in the 4 groups received saline, microecosystem regulator, fructose and traditional Chinese medicine, respectively, for 5 days. Samples of feces of the mice were taken after the completion of experiment and the amount of bacterial floras were assessed with the same procedure as in the first experiment. Results 1 The model of intestinal flora regulation:Compared with the control group using saline, all the 3 experimental groups showed that bifidobacterium, lactobacillus floras and intestinal bacillus were significantly increased after treatment with the microecosystem regulator, fructose and traditional Chinese medicine, respectively. 2 The model of intestinal flora imbalance prevention: Compared with the control group using normal saline, the bifidobacterium, lactobacillus floras and intestinal bacillus were significantly increased in the 3 experimental groups after treatment with microecosystem regulator, fructose and traditional Chinese medicine, respectively. Conclusion The microecosystem regulator, fructose and traditional Chinese medicine may excert effects to prevent the intestinal flora imbalance and increase the bifidobacterium, lactobacillus and intestinal bacillus floras in the intestine.

Abstract:Objective To study the catheterization and angiography of hepatic artery via carotid artery route in rats. Methods Cervical and abdominal incisions were made on 60 male Sprague Dawley rats. At the open surgery a catheter was inserted into the hepatic artery via left common carotid artery, and then a hepatic artery angiography was performed. Results The success rates of catheterization and angiography were 95% and 100%, respectively. Conclusion The catheterization and angiography of hepatic artery via carotid artery route in rats is a feasible approach with a rather high success rate of technique and a popular future.

Abstract:Transgenic technology has been a new method for research of animal models of human diseases. This article is a review on the development of transgenic animal models of human diseases such as neural diseases, tumors, cardiovascular diseases, dermatoses, infections, etc.

Abstract:Obesity may occur after long period disorders in appetite and energy metabolism regulations which are controlled by some specific biological factors. Over the past 10 years, great progress has been made in understanding of the pathophysiology of obesity and the interactions between genetic predisposition and environment to weight gain and experimental studies on obesity animal modeds as well.

Abstract:With the development of molecular biology, more and more kinds of molecular libraries have been applied in the research of serodiagnostic reagents for infectious diseases. The molecular library technology has special values compared with conventional techniques for serodiagnostic screening of antigens, especially when the pathogens are difficult to culture artificially or the specific antigen purification is hard. In the present paper the application of DNA library, phage display random peptide library, phage antibody library, etc. in the research of serodiagnostic reagents and their special values were reviewed.

Abstract:主要组织相容性复合体(MHC)不仅与移植排斥有关，而且广泛参与免疫应答的诱导和调节，所以具有很重要的免疫学意义。本文介绍了MHCⅠ类和MHCⅡ类分子结合的多肽分析，及MHC在异种移植中与T细胞、NK细胞的相互作用，MHC基因转移诱导免疫耐受和基因治疗等方面的最新研究进展。

Abstract:Pathogenic Mycoplasmas have been found in humans, animals, plants and insects,which can cause many diseases. However, the diagnosis of Mycoplasmas with culture and serological methods is limited, due to slow growth, extensive nutritional requirements and cross antigens of the pathogens. In recent years, with the progress of molecular biology, polymerase chain reaction (PCR) has been adapted widely for the diagnosis of Mycoplasma infections. This review mainly summarizes the successful application of PCR techniques, such as standard PCR, PCR ELISA, nested PCR, real time quantitative PCR, RT PCR and multiplex PCR to the diagnosis of Mycoplasmas . These techniques provide timesaving, sensitive, specialized methods in Mycoplasmas detection.