Abstract:目的 建立兔肝癌裸鼠模型，为兔肝癌可移植模型的建立奠定基础。方法 利用二乙基亚硝胺诱发日本大耳白兔肝癌，将获得的肝细胞癌接种于裸鼠皮下，并反复传代，将传代瘤块经体外培养后回植于裸鼠皮下，并继续在裸鼠体内传代。结果 肝细胞癌组织前5代对裸鼠致瘤率约40％；经体外传代后移植瘤成活率100％，潜伏期7～10d，无自然消退，无转移。病理检查证实该移植瘤保持着兔肝细胞癌特征，肝细胞抗原阳性，甲胎蛋白阳性。结论兔肝部细胞经传代和体外培养可增加移植成功率，并可缩短时间。

Abstract:Objective To evaluate the optimal anesthesia in animal experiments. Methods Several animal species ware used in this study: mice, rats, guinea pigs, rabbits, dogs, cats, pigs and gouts. Combined anesthesia was tested on several animal species, in different ways and with different anesthetics, and the results were evaluated. Results The combination of ketamine hydrocholoride, sumianxin and diazepam in a ratio of 4:2:1 was found to be an optimal intravenous anesthetic agent. For canine, feline and swine, combined anesthesia of both intravenous and intramuscular was most satisfactory. Combined use of atropine, which is an anticholinergic agent, can effectively avoid overanesthesia. Conclusion Satisfactory results of anesthesia in experimental animals can be obtained with an appropriate combined anesthesia.

Abstract:Objective To evaluate the effect of genistein on metabolism index in diabetic mellitus(DM) rats,including blood sugar, serum insulin, serum lipid, renal function, etc, and their kidney pathology. Methods 45 healthy male Spraque-Dawley rats were divided into control group (10 rats,free diet),DM group (four weeks:9;eight weeks:8; free diet),DM Gen group (four weeks:10; eight weeks:8; free diet gensitein of 30 mg/kg/day). DM model was made by intraperitoneal injection of streptozotocin(60 mg/kg).Four weeks and eight weeks after, the rats were fasted for 12 hours,then were killed. Blood sugar, serum insulin, serum lipid, renal function were assayed; rat kidneys were examined histopathologically. Results Genistein could decrease the level of blood sugar, cholesterol, improve insulin sensitivity, and also could decrease the level of urea nitrogen and creatinine. Besides, genistein could relieve glomerulus hypertrophy and renal tubular dilatation. Conclusions Genistein can improve the glyeolipid metabolism disorder of diabetic mellitus rats,relieve the changes of renal pathology, improve renal function,and delay the progression of diabetic nephropathy.

Abstract:目的探讨建立人急性早幼粒细胞白血病小鼠模型的有效方法。方法将4～6周SCID鼠随机分成A、B两组,A组为非照射组、B组为照射组,两组均为每只尾静脉注射2×106HL-60细胞株,每周检测小鼠外周血白细胞计数、外周血涂片人白血病细胞阳性率、病理组织学检测小鼠瘤细胞弥散生长浸润肝、脾等器官情况。结果注射3周后两组外周血白细胞计数两组差异无显著性。但外周血涂片人白血病早幼粒细胞细胞阳性率分别为8.3%、5.4%,B组高于A组(P<0.05)。而病理组织学证实两组小鼠注射瘤细胞28d后均有器官浸润如肝、脾、肾肺等,且B组远较A组广泛而严重。结论照射组和非照射组Scid小鼠静脉2×106HL-60细胞株均能建成人类粒细胞白血病小鼠模型。照射组更能模拟临床白血病累及骨髓和弥漫生长特点,是一个体内研究人类白血病发病机理及实验治疗良好的模型。

Abstract:Objective To establish a hyperuricemic renal impairment model in rats. Methods High, moderate and low dose of adenine and ethambutol were used to Wistar rats. Concentration of serum uric acid and urine nitrogen and creatinine was measured before and after treatment. The histopathological and uhrastructural changes were examined by light and electron microscopy. Results Serum uric acid was increased in the high and moderate dose groups, and the high dose group showed the most severe lesions. Conclusion The moderate dose treatment is suitable for preparation of hyperuricemic renal impairment model in rats.

Abstract:Objective To compare the reactivity of imported and domestic monoclonal antibodies against H-2 antigens of inbred strain mouse. Methods The percentage of quantity of obituary cells (positive cells) was acquired by using microcytotoxicity test. Result The imported antibodies H-2D~b(27-11-13), H-2D~d(34-5-8S), H-2D~k(15-5-5.3), H-2K~k(16-3.22.4) reacted to the homologous antigens and the death rate of the spleen cells was more than 60%. The domestic antibodies H-2D~b(2), H-2D~d(4), H-2D~k(32), H-2K~b(33), H-2K~d(31), H-2K~k(23) induced death rate of spleen cells was also over 60%. The reaction of both imported and domestic monoclonal antibodies against H-2 antigens of inbred strain mouse was in consistency. Conclusion The domestic monoclonal antibodies against H-2 antigens of inbred strain mouse can also be applied to monitor the mouse's H-2D and H-2K in mice.

Abstract:目的 依照造成骨质疏松症的骨质中成骨细胞及破骨细胞的失衡机制对去卵巢大鼠模型骨质中的成骨细胞、破骨细胞的数量变化进行研究。方法 将60只雌性SD大鼠随机分为A、B、C、D、E组，A组大鼠做假性去卵巢（Sham），B、C、D、E组大鼠摘除双侧卵巢（OVX）。14周后，A、B组大鼠注射生理盐水，C组大鼠注射谷慷太林，D组大鼠注射苯甲酸雌二醇，E组大鼠联合用谷慷太林和苯甲酸雌二醇，持续30d后在光学显微镜下观察松质骨区成骨细胞、破骨细胞数量变化。结果 E组与A、B、C、D组成骨细胞数量均数两两比较，C组与A、B、D组成骨细胞数量均数两两比较，组间差异具有统计学意义；A、B、D组成骨细胞数量均数两两比较，组间差异无统计学意义；B组与A、C、D、E组破骨细胞数量均数两两比较，组间差异具有统计学意义。A、C、D、E组破骨细胞数量均数两两比较，组间差异无统计学意义。结论 谷慷太林与雌激素联合应用对成骨细胞数量增加作用及对体内缺乏雌激素的破骨细胞数量降低作用显著。

Abstract:Objective To investigate the expression of heat shock protein (HSP) 27, 70 and 90 in Microtus fortis ovarian cancer. Methods Eight cases of spontaneous ovarian cancer and normal ovary were examined by histopathology and assessed for the expression of HSP27, HSP70 and HSP90 using immunohistochemical SP technique. Results All the 8 tumors were histopathologically diagnosed as serous cystadenocarcinoma. The positive-staining rates of HSP27, HSP70 and HSP90 in the cancers were 100%, 100% and 62%, respectively, while all were negative in normal ovary tissue. Conclusion The results demonstrate that HSP27 and HSP70 are overexpressed in the spontaneous ovarian cancers of Microtus Fortis, and the expression of HSP90 is lower. It is worthy of further study whether an animal model of ovarian cancer can be established from such tumor-bearing Microtus Fortis and to serve experimental studies on ovarian cancers.

Abstract:Objective To investigate the cardioprotective effects of delayed preconditioning of allitridium on experimental acute myocardial infarction in rabbits. Methods Rabbits were pretreated using allitridum (1 mg/Kg or 0.5 mg/Kg). The percentage of the weight at infarction and at risk (I/R), the myocardial morphological changes were measured 24 h later to determine the protective effects against experimental ischemia/reperfussion injury. Results It was observed in hearts treated with 1 mg/Kg allitridium that I/R area decreased 30.62% and the ultrastructure was obviously improved than the compared group. Conclusion Allitriuium can mimic the delayed cardioprotection on experimental acute myocardial infarction in rabbits. Allitridium has potential value in treating coronary heart diseases.

Abstract:目的 应用基质金属蛋白酶抑制剂1，10-菲罗啉作用于妊娠大鼠造成流产的模型，以验证基质金属蛋白酶在正常妊娠中的作用。方法 妊娠Wistar大鼠随机分为4组，A组腹腔注射生理盐水作为对照组，B、C、D组分别于妊娠早期、妊娠中期及妊娠全程腹腔注射基质金属蛋白酶抑制剂1，10-菲罗啉，各组例数均为10例。于受孕21．5d剖宫产，统计胎仔数、胚胎丢失数及流产率，并分别称取胎仔体重和胎盘重量，RT—PCR检测胎盘基质金属蛋白酶9（MMP-9）mRNA的表达。结果 基质金属蛋白酶抑制剂1，10-菲罗啉能导致大鼠胚胎丢失，自B组至D组流产率逐渐增高，并且C组和D组胎仔体重明显低于对照组，RT-PCR亦证实孕鼠腹腔注射1，10-菲罗啉后其胎盘MMP-9mRNA的表达显著下降，其下降程度与1，10-菲罗啉的干预时限成正比。结论 基质金属蛋白酶抑制剂在妊娠期抑制MMPs的表达，使MMPs的分泌不足，导致流产或胎鼠宫内生长迟缓，提示MMPs在胎盘滋养细胞浸润和妊娠的维持中具有重要作用。

Abstract:目的 本研究用光学显微镜观察新西兰雌性白兔阴道的正常组织学及药物刺激后所发生的组织病理学改变。方法 新西兰兔子宫、阴道分离后固定于11％甲醛内，常规脱水、透明、石蜡包埋，制片，HE染色，光学显微镜观察。结果与讨论 新西兰雌性白兔阴道是一根较粗的肌膜性管道，延续于子宫颈，下接阴道前庭，阴道黏膜表面主要被覆单层柱状纤毛上皮，阴道前庭黏膜表面被覆未角化的复层鳞状上皮。在药物刺激下，阴道上皮可发生坏死、脱落，继而形成糜烂或溃疡，局部可有肉芽组织形成及炎细胞浸润。根据病变程度可进行半定量计分。

Abstract:Gymnocypris Przewalskii is one of the endemic fishes in Qinghai lake. In order to provide a basis for formulating reasonable breeding protection measures, a series of investigation have been done since 1970. The works referred to its geographical distribution, habits, morphology, taxonomy, reproduction, ontogenesis, growth, food, karyotype, isozymes, breed and care and breeding technical specifications, etc. According to those reports, the author advanced some proposals about future application of this characteristic fish in laboratory animal science.

Abstract:Objective To establish the methods for the detection of antibody to mouse Toxoplasmosis. Method Antigen and positive serum of Toxoplasmosis were prepared, and the conditions of IFA and ELISA determined. Two pairs of primers were designed and synthesized based on the sequence of Toxoplasma gondii B1,and the PCR was constructed. Result The positive rates detected by IFA and ELISA were significantly higher than that by indirect haemagglutination (IHA) and antibody titres in ELISA were higher than in IFA. Conclus The IFA and ELISA are the effective methods for detection of mouse Toxoplasmosis with high sensitivity,and PCR can detect Toxoplasma gondii DNA and diagnose toxolpasmosis with high specificity and sensitivity.

Abstract:Objective To establish a route for administration of drugs to liver directly and reduce their influence on other organs. Methods A catheter was inserted in proper hepatic artery and remained one end of it outside under surgical operation microscope. Results The total number of rats in experiment was fifteen. Ten of them were alive and recovered from operation quickly. The reasons of death were vascular injury, liver damage, overdose of beparin and accident in anesthesia, which could be avoided by skilled operaters. Conclusion The above-mentioned method is simple, and easy to be mastered when drugs are given through the free end of the catheter.

Abstract:Objective To evaluate the reliability of enzyme immunoassay (EIA) of HSV-1 as antigen for detection of B virus antibodies. Methods B virus antibodies were detected with enzyme immunoassay (EIA,HSV-1antigen),enzyme-linked immunosorbent assay (ELISA, HSV-1antigen), immunobinding assay (DIA ,B virus antigen), immunofluorecence assay (IFA, B virus antigen) and enzyme-linked immunosorbent assay (ELISA, B virus antigen) in macaque monkey sera. The testing results were compared and analysed. Results Consistency of testing results of EIA(HSV-1antigen)with DIA( B virus antigen), IFA (B virus antigen) and ELISA (B virus antigen)were 100%,95.6% and 97.4%, respectively. The negative sera which were confirmed by EIA (HSV-1antigen) were still negative by IFA( B virus antigen). Among 779 negative sera confirmed by EIA (HSV-1antigen), 2.6%(20) was positive by ELISA (B virus antigen). Conclusion There is much consistency of testing results of EIA(HSV-1antigen)with DIA(B virus antigen), IFA(B virus antigen)and ELISA(B virus antigen). Meanwhile, among B virus free animals which were confirmed using HSV-1antigen, very few animals would be positive using B virus antigen.

Abstract:B virus is one of P4 zoonotic agent, which can cause fatal encephalomyelitis in humans. The virus naturally infects macaque monkeys, resulting asymptomatic or mild in macaques, but it can be fatal in humans. B virus disease in humans is usually attributed to animal bites, scratches, or percutaneous inoculations with infected materials. The survival rate will increase if prevention and therapeutic measures are taken in time after infected by B virus. This paper reviews the prevention and therapeutic methods for BV infection.

Abstract:Microsatellites, simple sequence repeats (SSR), are distributed throughout the eukaryote genomes. With abundant number, highly polymorphic character, reliable outcome, microsatellites are useful tools for gene mapping construction, localization of quantitative trait loci, marker assisted selection, genetic diversity detecting, genetic monitoring and others.

Abstract:There are three types of laboratory animal diets in biomedical researches, including natural- ingredient diets, purified diets and chemically defined diets. There are variations in the composition of diets from different manufactures and different batches of one brand. The variation of the diet composition and the feeding regimes can affect the health status and metabolism of experiment animals, and enhance or mask the response of animals to a given stimulus, which can adversely affect the accuracy and precision of experiment results. As part of an experiment food intake may differ between control and experiment groups. This can create biased results and lead to a false interpretation of the data.

Abstract:Recent studies of Neisseria gonococci infection in mouse have yielded important progress in the pathogenesis of Neisseria gonococci. Estrogens have an anti-inflammatory effect. Estradiol-treated mouse may be infected from Neisseria gonococci. This Neisseria gonococci infection mouse model treated with estradiol may serve as a useful tool for ascertaining gonococcal pathogenesis, evaluating potential gonococcal vaccine candidates, screening and estimating bactericide.