Abstract:Objective To investigate the protective effect of recovered SARS monkeys and vaccinated monkeys on the Infection of SARS-CoV. Methods To analyze changes of anti-SARA-CoV neutralization antibody, viral detection and pathological changes in lungs of monkeys from groups of SARS recovered monkeys, vaccinated monkeys and SARS model monkeys. Results Groups of SARS recovered monkeys, vaccinated monkeys produced high titer of neutralization antibodies, and inhibited the replication of the virus to a certain extent. The pathological changes of groups of SARS recovered monkeys and vaccinated monkeys were lighter than that in the SARS-monkey group. Conclusions Both groups of SARS recovered monkeys and vaccinated monkeys showed resistance to infection of SARS-CoV

Abstract:Objective To establish an osteosarcoma model of rat and to study its biological characteristics.Methods Intact tumor pieces were inoculated into the right tibia of rat,the rats were killed in different time and observed the growth of tumor and metastasis.At the same time,serum alkaline phosphatase was detected.Result The tumor growth rate was 91.67%,from the first week to the second week,the tumor grew out from medullary cavity,then it widely destroyed cortical bone and invaded soft tissue on third week but there was no pulmonary metastasis.Pulmonary metastasis took place on the fourth week,on the fifth week the cortical bone was destroyed more obviously than before and the quantity of pulmonary metastasis increased,and the rat finally died for respiratory failure on the sixth or seventh week.The level of ALP began to step up from the first week to the second week,reached a peak on the third week.It fell off from the fourth week to the sixth week,and approached a platform on the seventh week which was still higher than normal level of same time.Conclusion The tumor growth rate is high,and the animal is available easily.So the model can be widely used for scientific research.The model simulated the clinical process of human osteosarcoma,and could be a satisfactory model for studying osteosarcoma.

Abstract:目的探讨通过清醒膀胱测压建立梗阻性逼尿肌不稳定(detrusor instability,DI)模型的方法。方法成年雌性Wistar大鼠于会阴部行不完全性尿道结扎制作尿道出口梗阻模型,6周后耻骨上膀胱造瘘,于清醒状态下行充盈性膀胱测压,观察膀胱容量、压力变化、排尿反射及逼尿肌不稳定的发生情况。结果大鼠清醒测压时保持排尿反射,梗阻组发生逼尿肌不稳定的占76.17%,单位时间发生率(1.38±0.80)次/min,逼尿肌不稳定组膀胱最大压力(93.62±12.91)cmH2O和容量(1.20±0.41)mL较对照组(51.50±8.78)cmH2O(、0.33±0.08)mL显著增高(P<0.01)。结论清醒大鼠膀胱测压是建立梗阻性逼尿肌不稳定模型的可靠和实用的方法。

Abstract:目的为制备FVB转基因小鼠及保种繁殖提供最佳超排周龄及胚胎冷冻方法。方法对4—6周、6．8周、8．10周FVB小鼠进行超数排卵效果的比较及采用体外培养受精卵至2-cell法和输卵管吹卵法两种不同方法收集2-cell受精卵进行胚胎冷冻。结果4．6周雌鼠经超排后回收的受精卵（24．6个）明显高于6—8周龄、8—10周龄的FVB雌鼠（14．5个、16．2个），差异有显著性；两种方法回收的2-cell受精卵经冷冻保存后，Ⅱ组受精卵解冻后2-cell形态正常的胚数（106个）高于I组解冻后2-cell形态正常的胚数（90个），但两者无显著性差异。结论本研究将为制备FVB转基因小鼠及转基因动物的种系保存和繁殖提供了基础。

Abstract:目的观察大鼠短暂性全脑缺血再灌注前后动脉血气、乳酸的变化。方法选用12只清洁级健康sD大鼠，根据Smith等创建的“双侧颈总动脉阻断＋低血压法”建立大鼠短暂性全脑缺血模型。在双侧颈总动脉阻断前及开放后10min，从股动脉抽取0．5mL血样，进行动脉血血气、乳酸测定。结果大鼠全脑缺血再灌注10min后的动脉血pH值、BE、HCO3^-显著降低，乳酸值显著升高（P〈0．05）。结论大鼠短暂性全脑缺血再灌注后早期可发生乳酸代谢性酸中毒，可能是导致缺血性脑损伤病理生理机制的重要环节。

Abstract:Objective To establish an platform to investigate embryos of KM mice. Methods The sperm corned from from transgenic mice of GFP fertilized and cuhuredin vitro with the ovum of KM mice getted by superovulation. The embryo was observed under fluorescence microscope. Results Sperm of GFP mice was no expression of green fluorescence in fluorescence microscope, while when fertilized with the KM females in vitro, the oocytes took on strong or weak expession of green fluorescent. Conclusion Strong or weak expression of green fluorescent could be the mark of embryos viability.

Abstract:Objective To observe the effects of jiangtangtongmai granule on alloxan-induced diabetes in rabbits.Methods Blood glucose,insulin,glucagons,superoxide dismutase(SOD) and malondialdedyeds(MDA) were measured through the Alloxan-induced diabetic model of rabbits.Results Jiangtangtongmai granule could significantly reduce blood glucose of alloxan-induced diabetes of rabbits,increase insulin level,reduce glucagon contents,enhance SOD activity and reduce MDA contents.Conclusions Jiangtangtongmai granule can obviously improve the alloxan-induced diabetes of rabbits.

Abstract:Objective To establish an ischemia model in gerbil and investigate the changes of SOD and MDA concentration in the brain of ischemic animal.Method 72 adult gerbils were chosen and right common carotid artery were ligated to make cerebral ischemia model.The animals died with cerebral ischemia or anesthetized were anatomized.The vessels on the ventral surface of the brain were studied under a microscope and the content of water,SOD and MDA in brain were determined.Results Anatamization results show that there are 4 anatomical patterns of anterior communication arteries(ACA) and 3 anatomical patterns of posterior communication arteries(PCoA) in 72 experimental gerbils.The value of MDA and SOD in ischemic gerbils are all higher than that in control and sham operation animals.Conclusion Cerebral ischemic model of Mongolian gerbil can be made by ligating right common carotid artery and the change of SOD and MDA in this model is different from the model of ischemia-reperfusion.

Abstract:Objective To explore the change of the anti-oxidation in plasma in vascular calcification rats.Methods The male Sprauge Dawley rats were randomly divided into 2 groups,one of the group were induced vascular calcification.Von Kossa stanning in aorta were observed and the levels of the alkaline phosphatase and calcium content in aorta were detected,SOD,MDA levels and total anti-oxidation ability in plasma were also detected after six weeks.Results Many black granules among the smooth muscle cells and its matrix in calcified aorta by Von Kossa stanning in VDN group was observed,102.7%,259% increase ALP and calcium content were observed in VDN group than that in control group(P<0.01).584.1% increase MDA,26.2% and 67.4% decrease SOD and total anti-oxidation ability were observed in VDN group than that in control group(P<0.01).Conclusion Anti-oxidation ability in plasma was decreased in vascular calcification rats.

Abstract:Objective To prepare BaMa minipig's acellular dermal substitute in order to ultilize it into xeno-transplant in clinical use.Methods Epidermis of BaMa minipig's skin was removed by 0.1% dispaseII or 0.25% typsin for one night,then dermis was treated with 0.5% triton X-100 for 24h,succussed or not succussed.The prepared acellular dermal matrix was examined macroscopically with gross observation,light microscopy,bacteris culture test and cytotoxicity test.Results Epideris could be removed thoroughly either in 0.1% dispaseII or 0.25% typsin for one night,but the basal memberane was integrity in 0.25% typsin and in 0.1% dispaseII the basal memberane was not integrity.Cellular components were elimated thoroughly treated by succuss in 0.5% triton X-100 for 24h.There were not any bacteria and ctyotoxity.Conclusion Prepared BaMa minipig's acellular dermal substitute had no cellular components,bacteria and cytotoxity,which would be an ideal dermal substitute.

Abstract:目的在相对稳定的饲养环境下，观察C57BL／KsJ杂合仔（＋／db）小鼠的生长繁殖能力，同时对纯合仔（db／db）、杂合仔（＋／db）小鼠进行部分生化指标的测定。方法将20对＋／db小鼠饲养在层流柜中，进行繁殖试验，记录胎间隔、产仔数、离乳数，测周重并采血进行血糖、胆固醇、甘油三酯、血胰岛素的测定。结果从6周开始纯合仔与杂合仔小鼠的体重出现了明显的差异（P〈0．01），db／db小鼠的体重明显高于＋／db小鼠的体重；db／db小鼠的血糖较＋／db小鼠明显升高（P〈0．01），在19周时db／db小鼠的血糖、血肌酐达高峰，15周时血胰岛素达高峰。结论该小鼠在我科繁育成功，纯合仔表现出Ⅱ型糖尿病的特征，且高血糖已造成肾脏一定程度的损伤。

Abstract:目的观察弱毒株弓形虫慢性感染对雌性KM小鼠生育能力的影响。方法用弓形虫AH株人工感染性成熟的雌性KM小鼠,40 d后做交配试验,观察其受孕率、产仔数及生殖器官的病理变化。结果被感染小鼠的受孕率为70%,平均产仔数为(8.8±3.1)只/窝;正常对照小鼠的受孕率为100%,平均产仔数为(12.3±1.3)只/窝。两组动物窝产仔数有极显著差异(P<0.01),被感染小鼠生殖器官有不同程度的损伤。结论弓形虫慢性感染使雌性小鼠生殖器官遭受一定程度的损伤从而使小鼠生育能力显著下降。

Abstract:目的建立SYBR GreenⅠ荧光染料实时定量PCR方法，测定猴免疫缺陷病毒（SIV）前病毒DNA。方法巢式RT-PCR扩增SIVmac251病毒RNA gag基因上1360-1837之间的长度为477bp的片段，将该片段克隆到pGEMT载体上，构建pGEM-SIV gag477质粒。该质粒经大量扩增纯化后定量，10倍系列稀释后，做出标准曲线，作为SIV前病毒DNA荧光定量检测的外标准品。结果应用Roche公司FastStart DNA Master SYBR Green Ⅰ Kit，该标准品可精确定量到10copies／μL。结论制备的pGEM-SIV gag477质粒外标准品纯度高，SYBR GreenⅠ荧光染料实时定量PCR法特异性、敏感性高，稳定性好，可用于定量测定猴免疫缺陷病毒（SIV）前病毒DNA载量。

Abstract:目的根据实验需要，选择合适的实验动物，提供可操作性强的采集脑脊液的方法。方法通过对兔和大鼠小脑延髓池的解剖观察，确定抽取脑脊液的最佳进针部位与穿刺方向。结果兔和大鼠小脑延髓外被硬脑膜覆盖的区域膜薄质软易刺破，投射到颈部表皮是在枕骨隆凸与第一颈椎之间，将兔和大鼠的头尽量向胸部屈曲后测得的最佳进针部位分别位于枕骨隆凸正中下1．0～1．2cm和0．6～0．7cm。经皮肤穿过硬脑膜进入延髓池，针平行头部弯曲角度穿入，不易伤及延脑和血管。经过对兔和大鼠小脑延髓池的解剖定位，采集脑脊液的成功率明显高于实验前（P〈0．01）。结论准确的穿刺定位是采集实验动物脑脊液的关键。

Abstract:Objective To study a new treatment for bacillary dysentery of infant in rhesus monkeys.Methods\ From October 2002 to October 2004,we treated the part diseased infants by keeping clysis with a compound medicines of sulfamethoxazole,cefoperazone and dexamethasone,at the same time,we sterilized cages and surrounding by tolyl hydroxide soap and Puritabs Maxi in order to prevent transmission of the disease.Results\ It showed that the most evident effective rate was 67.9%(38/58);the evident effective rate was 23.2%(13/58);the rate of no effect was 8.92%(5/58);the mortality was 5.4%(3/58).Conclusion\ we concluded that this treatment can enhance the healing rate for the Bacillary dysentery of infant and reduce side effect of medicines,as compare with the former therapeutic methods.

Abstract:Objective To perform Caesarean Section to save the dystocia monkeys. Method The monkeys were anaesthetized by ketamine hydrochloride intramuscularly before surgery. Vitamin C was supplied and disinfected treatment was afforded in post-surgical care. Infant monkeys were fostered by artificial care. Results Total fifteen cases were performed and the successful rate was 100%. All mother-monkeys survived well and 70% infants could survive and grow up healthy. Conclusion Using ketamine hydrochloride to anaesthetize could fit for the time and stability demand of caesarean section in Rhesus monkey, no complications and accident happened in 15 operated-monkeys. The caesarean section method not only would be propitious to the protection the Rhesus monkey, but also would reduce the death of monkeys caused by the dystocia.

Abstract:In order to assure the accuracy of the diuretic pharmacology experiment, a screening procedure play an important role in improving the uniformity of experimental animals. At present, the screening method designed by Aston Roy is used widely in our country. In abroad, however, the method designed by Kau is popular. The latter is suitable for diuretic pharmacology of the traditional Chinese medicine, because of its merits such as smaller numbers of sample,reproducible,longer period and so on. It is concluded that this method should be applied to test animals screening procedure of the Chinese herb pharmacology experiment.

Abstract:N-糖基化是机体蛋白质翻译后的一种重要的修饰过程。蛋白质N-糖基化生物过程和相关的许多酶主要定位于胞浆内质网和高尔基体。α-甘露糖苷酶是一种参与糖蛋白合成和代谢的重要蛋白酶，它的功能是修剪N-聚糖中的甘露糖。本文重点介绍α-甘露糖苷酶的分类、在体内参与糖蛋白的成熟和降解过程，以及α-甘露糖苷酶基因突变可导致的相关疾病。