Abstract:

Abstract:目的建立稳定表达bcr-abl融合基因的、可移植的小鼠肿瘤细胞系,解决慢性粒细胞白血病疫苗研究的瓶颈问题。方法从重组克隆载体pGEMbcr-abl中酶切出bcr-abl融合基因片段,并将其亚克隆进反转录病毒载体pLXSN中。脂质体介导重组反转录病毒载体pLXSNbcr-abl转染包装细胞PT67,G418筛选后获得稳定产病毒的包装细胞。收集病毒感染NIH/3T3细胞,加G418筛选后进行反转录病毒滴度测定,计算病毒效价为2×107CFU/mL。收集病毒上清感染SP2/0细胞(H-2d),经G418筛选获得稳定表达bcr-abl融合基因片段的SP2/0细胞株。然后用SP2/0/bcr-abl细胞攻击同品系BALB/c小鼠,观察SP2/0/bcr-abl移植瘤生长的情况。结果经特异性PCR扩增和RT-PCR反应扩增,从基因组整合和基因表达水平证实获得了能稳定表达bcr-abl融合基因片段的鼠SP2/0细胞系。SP2/0/bcr-abl细胞能够在同品系小鼠体内形成移植肿瘤。结论该表达bcr-abl融合基因片段的小鼠肿瘤细胞系将作为研究bcr-abl基因疫苗的有效实验工具,为检验bcr-abl基因疫苗激发的小鼠CTL应答研究奠定物质基础。

Abstract:Objective To observe the expression of perforin and granzyme B mRNA in peripheral blood lymphocytes dynamically after heart transplantation in rats,and to assess the relationship between their expression level and acute cardiac allograft rejection.Methods The donor's heart and up-right lung were transplanted into the recipient's abdomen by the donor ascending aorta anastomized with recipient abdominal artery.The rats were divided into two groups after operation,the experimental group(n = 17),the donors were Wistar and the recipients were SD rats.The control group(n = 18),the transplantation was performed just between SD rats.FQ-PCR was used to quantify the perforin and granzyme B mRNA level in peripheral blood lymphocytes at varying times,meanwhile the graft was harvested to examine the pathological changes.Results At 24 hours after transplantation,the expression of perforin and granzyme B mRNA began to be up-regulated,reaching the peak at 3 to 5 days,and still kept a high level at 7-11 days.There was a significant difference in comparison with the level before operation and the expression level of control group(P<0.001).Those changes occurred earlier than the pathological changes.No changes were observed in the control group.Conclusion The graft rejection is concomitant with the expression of perforin and granzyme B mRNA.The expression level of perforin and granzyme B mRNA is changed earlier than pathological changes.The measurement of perforin and granzyme B mRNA may be a good indicator of acute rejection.

Abstract:Objective To assess and analyze the levels of estradial(E_2),progesterone(P),follicle-stimulating hormone(FSH) and luteinizing hormone(LH) in blood serum of Fmr1 knockout mice and FVB mice before and after superovulation.Method The levels of E_2,P,FSH and LH were measured by RIA and statistically analyzed.Results The levels of FSH and LH were not different between Fmr1 knockout mice and FVB mice.The levels of E_2 and P were higher in FVB mice(P<0.01).The levels of E_2,P,FSH,LH and the amount of ova had no significant difference between Fmr1 knockout mice and FVB mice at 10 weeks of age after superovulation(P>0.05),but the amount of ova of superovulated Fmr1 knockout mice 10 weeks old was higher than those in 6 weeks old(P>0.05).Conclusion Fmr1 gene may perhaps influence the E_2,P,FSH and LH levels in serum.It should be taken into consideration in mouse reproductive physiological studies.

Abstract:目的通过对易卒中自发性高血压大鼠(SHR/SP)饲喂加味补阳还五汤,观察其对SHR/SP大脑海马区一氧化氮(NO)含量的影响,从而证实加味补阳还五汤能降低SHR/SP大脑海马区本NO含量。方法将20只4周龄雄性易卒中自发性高血压大鼠(SHR/SP)分成2组(每组10只),其中一组喂含补阳还五汤的饲料为用药组,另一组常规饲养,作为对照组。同时用同龄10只WKY大鼠作为空白组。实验周期为3个月,观察大鼠海马区NO含量变化。结果空白组的海马区NO含量(4.56±0.57)μmol/L明显低于用药组(8.78±1.34)μmol/L(P<0.01),而用药组(8.78±1.34)μmol/L的海马区NO含量又明显低于对照组(10.10±1.09)μmol/L(P<0.01)。结论补阳还五汤可降低SHR/SP大鼠海马区的NO水平。

Abstract:Objective To establish a RT-PCR method for detection of rabbit hemorrhagic disease virus (RHDV).Methods RHDV were purified from liver samples of rabbits died from RHDV infection,and total RNA was prepared.A 368 bp fragment was amplified by RT-PCR,then was cloned into pMEG-T vector.The recombinant plasmid was sequenced.Results The sequencing result of PCR products was homologous with the nucleotide sequence reported in the reference.The sensitivity assays indicated that 10~(-7) virus titer can be detected by PCR.Conclusion The established RT-PCR method for detection of RHDV is highly sensitive and specific.

Abstract:Objective Three different staining techniques were used to visualize S180 ascites smear morphology. The results were compared to find a best and simple staining technique showing most clear cytological characteristics. Method S180 ascites smear was stained by H.E. staining, Pap staining and fast staining, and the cell morphology was observed and compared under microscope. Results Among the 3 staining methods, the fast staining was most simple to operate and revealed cellular structures best. There was a best contrast among cytoplasm, nucleus and nucleolus in the smears stained by the fast staining, and the cell structures can be distinguished most clearly. Conclusion The fast staining is better than HE staining and Pap staining in S180 Aseites smear observation.

Abstract:目的测定新开发的实验动物垫料玉米秸、麦秸的物理性状及卫生指标,探索其定型生产的合理流程。方法对玉米秸、麦秸进行合理加工,测定并比较玉米秸、麦秸、刨花的物理性状、化学污染物含量及携带的微生物数量。结果3种垫料的化学污染物指标均低于我国实验动物配合饲料卫生标准的要求。经预真空高压灭菌后垫料中菌落总数、大肠菌群、沙门菌数、真菌数等微生物指标均小于我国配合饲料卫生标准的要求。经预真空高压灭菌后3种垫料刨花、麦秸、玉米秸的含水量平均为(7.52±0.15)%、(7.60±0.13)%、(7.28±0.13)%。吸湿性分别为(290.94±62.58)%(、414.44±81.21)%(、325.84±78.11)%。结论新开发的实验动物垫料麦秸、玉米秸合乎实验动物垫料的性能要求,适合用于啮齿类实验动物饲养。

Abstract:Objective To explore the influence of Sumianxin(SMX),a composite anesthetic,anesthesia on haemodynamics,hematology,blood biochemical and blood gas indexes in rabbit. Methods Arterial and venous blood samples of the rabbits were collected before and after anesthesia with SMX to analyze the changes of hematologic,blood biochemical and blood gas parameters.The SMX was injected at a dose of 0.2 mL/kg body weight into the hind leg muscles of the rabbits.Three minutes later,the rabbits became anesthized.The carotid artery was cannulated to monitor the arterial blood pressure in each rabbit.The respiration rate and heart rate were monitored by the non-invasive electrocardiogram monitor.15 minutes after anesthesia,the arterial and venous blood samples of the rabbits were drawn for hematology,blood biochemical and blood gas analyses.Result 1.Compared with those before anesthesia,MCHC,PCT increased,and RDW-CV decreased significantly after anesthesia(P<0.05).The changes of hematological parameters were not significantly(P>0.05).2.Compared with those before anesthesia,Cr decreased very significantly after anesthesia(P<0.01),GLU,CK,CK-MB increased significantly(P<0.05),Mg increased significantly(P<0.05),and P increased very significantly(P<0.01).Other indexes of blood biochemicals were not changed significantly(P>0.05).3.Compared with those before anesthesia,PCO_2 increased very significantly(P<0.01),TCO_2,Beecf and HCO~-_3 increased significantly(P<0.01),and K~ decreased significantly after anesthesia(P<0.05).Other indexes of blood gas analysis were not increased or decreased significantly(P>0.05).4.Compared with those before anesthesia,MAP,RR,HR decreased after anesthesia.Conclusion Hemodynamics,hematology,serum biochemicals and blood gas were significantly influenced by SMX anesthesia in rabbits.

Abstract:Objective To characterize the etiological pathogens of epidemic diarrhea in hamster infants. Methods To collect feces from the hamster infants and detect the possible viruses, bacteria or parasites. RNA electrophoresis for rotavirus identification was performed using a group A rotavirus diagnostic kit. Results No definite bacteria or parasites were detected. Group A rotavirus was not detected. RNA electrophoresis showed 11 bands in patterns resemble to that of group B rotavirus. Conclusion The epidemic diarrhea in hamster infants is caused by group B rotavirus.

Abstract:Objective To study the blood glucose changes in diabetic rats induced by alloxan monohydrate, and to assess the influence of blood glucose changes on research with this animal model. Methods The diabetic rat models were made by tail vein injection of aUoxan monohyclrate. The fasting and non-fasting blood glucose levels and urine glucose level were measured. Results The non-fasting blood glucose level became stable after 10 days and maintained a relatively high level for 120 days after aUoxan monohydrate administration. The fasting blood glucose level was varying continuously. Conclusion The diabetic rat models axe stable for four months at least. When this animal model is used to evaluate the therapeutic effect of a drug, the nonfasting, but not the fasting blood glucose level should be used as an indicator for observation.

Abstract:目的利用C57BL／6小鼠制备H-Y抗血清并分析。方法以纯系C57BL／6雄鼠脾细胞做为抗原，通过腹腔注射免疫雌鼠的方法制备H-Y抗血清。利用经典精子细胞毒实验和ELISA检测方法分别对两组抗血清检测。结果H-Y抗血清对鼠精子的平均工作效价为1／20—1／160，而ELISA检测抗血清的最高效价为11640，比精子细胞毒实验的检测结果高。结论对两种检测方法的结果进行分析，认为ELISA能准确判断出H-Y抗血清的特异性和效价，尤其在选择免疫效果较好的雌鼠方面有较高的参考价值。

Abstract:目的探讨光波．微波辐射技术在骨组织快速脱钙与免疫组化染色中的作用。方法用8％硝酸分别在单纯微波、单纯光波、光波,微波组合Ⅰ和光波-微波组合Ⅱ的条件下脱钙，同时以室温脱钙作为对照；应用光波-微波-LSAB免疫组织化学染色技术检测不同脱钙条件下ANP、CD31、CD34、5-HT、S-100、NF、GFAP和Vimentin的表达。结果8％硝酸室温脱钙时间最长，光波．微波组合脱钙时间最短；微波组、光波组、光波．微波组合Ⅰ组、Ⅱ组脱钙的免疫组化染色均明显比对照组好；光波．微波组合Ⅰ组、Ⅱ组脱钙的免疫组化染色均明显优于单纯微波、单纯光波法；光波-微波组合Ⅱ-LSAB免疫组织化学染色技术比常规免疫组织化学染色所用时间短、染色效果好。结论光波．微波辐射脱钙及免疫组化染色所用的时间明显缩短，染色效果好。

Abstract:目的研究不同组织嗜性的小鼠肝炎病毒（MHV）组合抗原检测抗体的间接ELISA技术。方法比较两种不同组织嗜性抗原和组合抗原为基础的检测MHV抗体水平。将呼吸型MHV-1、肠型MHV-JX和混合抗原包被酶标板后，检测不同MHV毒株感染抗体。结果两种抗原都能检测出相应的MHV抗体，但交互检测效果差；组合抗原能同时检测两种MHV抗体，组合抗原可以提高MHV感染的检出率。临床样品中呼吸型感染率40％-58％，呼吸型感染率22％-70％，两型的共感染率为为17％-30％。结论组合抗原可以用于MHV感染的诊断试剂。

Abstract:Objective Hepatic cirrhosis is a common severe disease among people. Animal models of hepatic cirrhosis may provide an important basis for studies on this disease. An appropriate animal model should be chosen according to the aim of research. In this paper several models of hepatic cirrhosis in rats were reviewed and their features were compared.

Abstract:The bone metabolism in animals persists for whole lifetime,including both bone formation and absorption.Calcium and phosphorus are important components in bone structure and dietary calcium,phosphorus and calcium:phosphorus ratio affect the bones metabolism directly.Vitamin D can also affect the bone metabolism directly,besides its effect on regulation the absorption and metabolism of calcium and phosphorus.This article reviews the recent progress in research on the influence of calcium,phosphorus and vitamin D on the bone metabolism in animals.

Abstract:Objective In this paper, recent advances in research on the effects of vitamine E on immunity, reproduction and development in humans and animals were reviewed. It was shown that proper amount of vitamin E may promote evidently cellular and humoral immune responses, while dietary vitamin E deficiency can lead to impaired immune function, reduce resistance to diseases and result in abnormality of reproduction and development in human beings and animals.