Abstract:目的研究骨髓干细胞动员治疗急性心肌梗死的可行性。方法24只新西兰大白兔随机分为实验组和对照组，结扎实验动物前降支制作急性心肌梗死模型。实验组动物心肌梗死后24h开始皮下注射重组人粒细胞集落刺激因子，100μg/Kg，连续注射10d；对照组动物心肌梗死后24h开始注射等量生理盐水，连续注射10d。检测实验动物外周血骨髓干细胞动员情况、心功能变化及心肌梗死区毛细血管密度。结果两组实验动物骨髓干细胞动员前外周血有核细胞计数与单个核细胞比例近乎相等，差别无统计学意义；实验组动物重组人粒细胞集落刺激因子皮下注射，外周血骨髓干细胞一般在5d左右达到峰值，外周血有核细胞计数（63．10±16．72）×10^9／L及单个核细胞比例（97．1±2．88）％明显高于动员前，同时亦明显高于对照组；两组实验动物心肌梗死模型术后4周左心室射血分数与术前相比明显降低，分别为（50．11±6．37）％vs（42．27±9．35）％，实验组高于对照组，差别有统计学意义。实验组心肌梗死区内有大量岛状存活心肌，毛细血管计数（3．68±0．78），HP，而对照组心肌梗死区内岛状存活心肌少，坏死心肌由均质瘢痕组织替代，毛细血管计数（1．38±0．87）／HP，对照组明显少于实验组，P〈0．05。结论重组人粒细胞集落刺激因子（100μg／kg）皮下注射可有效动员骨髓干细胞入外周血参与梗死心肌修复，增加心肌梗死区毛细血管密度，改善心功能。

Abstract:目的探讨制作羊急性心肌梗死模型的麻醉方法；观察结扎左冠状动脉后心肌损伤标志物浓度的变化。方法选用成年羊共16只，经剑突下入路结扎左冠状动脉。麻醉采用气管插管、静脉内全麻及机械通气。术中连续监测ECG、MAP、CVP等。结扎前、结扎后1、3、6、12、24h分别抽取静脉血，以检测脂肪酸结合蛋白、CK-MB浓度。结果麻醉时间76—145（100．83±16．18）min。异丙酚用量9—23（16．79±3．16）mg，Kg，阿曲库铵用量0．8—1．4（1．07±0．39）mg／Kg。拔管时间为12—25（15．63±9．75）min。死亡4只，存活12只，存活率为75％。左冠状动脉结扎后心律失常以室性心律失常及心动过缓为主，常伴有MAP下降及CVP升高。术中需用利多卡因的为7例，需用阿托品的5例，需用升压药的6例，使用肾上腺素的5例，心内除颤的4例。存活动物模型中，LAD结扎后1、3、6、12h的血清脂肪酸结合蛋白（FABP）浓度均明显升高。结扎后3、6、12、24h的血清磷酸肌酸激酶同功酶（CK-MB）浓度均明显升高ECG复查结果显示所有存活模型均有心肌缺血及梗死的表现。心导管检查示结扎部位血流完全中断，梗死区心肌运动均明显异常（收缩与舒张运动减弱），3例模型中存在室壁瘤。组织病检示典型心肌坏死表现。结论气管插管及异丙酚，阿曲库铵静脉全麻用于羊急性心肌梗死模型的制备，简便、有效、安全。术中积极处理心律失常及血流动力学紊乱是提高存活率的关键。

Abstract:目的以扫描电镜观察大鼠激素合并内毒素诱导股骨头缺血性坏死模型病理变化，为实验和临床研究提供标准化动物模型。方法雄性SD大鼠8只，尾静脉注射大肠埃希氏杆菌脂多糖（LPS，Lipopolysaccharide）10μg/kg（wt）一次，肌肉注射甲基强的松龙20mg／kg（wt）三次。另2只为空白对照。6周后处死，取股骨头，扫描电镜观察。结果造模大鼠股骨头表面失去小丘样结构，不光滑并有塌陷，呈蜂窝状改变。结论糖皮质激素合并内毒素可诱导出大鼠股骨头坏死早期病理变化。

Abstract:目的研究肝移植早期供肝缺血再灌注损伤的发生机理。方法供肝在4℃乳酸林格氏液中保存45min（对照组）、4h、6h后进行大鼠原位肝移植，术后1、2、4及24h分别取肝上下腔静脉血测肿瘤坏死因子α（TNF—α）活性，术后2h取供肝组织进行TNF-α免疫细胞化学定位，术后24h取供肝进行常规病理学检查。结果①在原位肝移植过程后早期肝后血TNF-α活性显著升高，且在血管再通后2h达到高峰（4h组为3．81U／mL，6h组为7．96U/mL），其升高程度与供肝的损伤程度有关；②6h组可见TNF-α阳性染色的多角形或梭形细胞，散在分布于肝窦中，4h组染色较弱，对照组无染色。结论冷存可使供肝枯否细胞激活，产生TNF-α参与供肝的损伤过程。

Abstract:目的探讨玻璃化冷冻保存对小鼠卵巢组织内卵泡形态及卵巢组织激素分泌功能的影响。方法将23只4周龄ICR雌鼠随机分为新鲜卵巢组、去势组和玻璃化冷冻组，应用乙二醇（EG）和二甲基亚砜（DMSO）玻璃化冷冻小鼠卵巢组织。冷冻前后取部分小鼠卵巢组织行组织学观察；同时将部分新鲜和复苏组织自体移植入小鼠肾被膜下。移植术后5d开始观察小鼠的动情周期，术后30d测定小鼠血清雌激素浓度。结果冻融卵巢组织内存活的原始卵泡和初级卵泡与新鲜组织无显著差异（P〉0．05）；而次级卵泡的存活率显著下降（P〈0．01）。移植术后玻璃化冷冻组和新鲜卵巢组小鼠均出现动情周期，两组动情周期出现时间无明显差异（P〉0．05）。移植术后第30天两组小鼠血清雌激素浓度无显著差异（P〉0．05）。结论玻璃化冷冻法可以较好地保存小鼠卵巢组织内的原始卵泡和初级卵泡，且不影响卵巢组织的激素分泌。

Abstract:目的研究HBV基因在已传20代BALB／c-TgN（HBV D型1．3）SWS458小鼠中复制和表达情况。方法采用PCR荧光定量检测法、ELISA检测和免疫组化的方法研究HBV基因在转基因小鼠体内的复制和表达情况。结果F20代BALB／c-TgN（HBVD型1．3）SWB458小鼠血清HBVDNA为（104—106）copy／mL，HBsAg表达量为（124．41±33．46）ng/mL，preS1和HBcAg的OD值分别为0．248±0．076和0．635±0．338。肝组织有HBsAg和HBcAg表达，且HBsAg为胞浆型，HBcAg为核型。结论经过筛选和培育，本转基因小鼠传至20代时HBV基因复制表达稳定，表明我们已经获得一个传代表达稳定的HBV转基因小鼠品系。

Abstract:Objective To create a new animal model for exploring restenosis after percutaneous stransluminal coronary angioplasty(PTCA).Method 24 male Sprague-Dawley big rats were randomly divided into 4 groups(n=6).The 2.0F balloon catheter injury was perfomed through the right femoral artery into the left common carotid artery of rats.Around 6 rats from each group were euthanized by exsanguination at appropriate time point.The injuried left common carotid arteries were carefully removed and harvested,then the pathological slides were prepared and stained for optical microscopic observation.Microscopic analyses and quantitation of morphologic parameters were performed using HMIAS-2000,which was a full-automatic computerized colorful imaging analysis system.Results The proliferation of vascular smooth muscle cells(VSMC) was spotted on the surface of vascular lumen at 5d after the rat underwent balloon-dilation injury of the left carotid artery.Artery intimal proliferation progressed gradually as time went on.28days after the operation,morphological analysis demonstrated that there were markedly differences in the area of neointima hyperplasia,the vascular wall collagen proliferation between all model groups and the sham-operated group.Conclusion This experiment successfully duplicated the clinical animal model of RS,the model method of vascular restenosis established by balloon injury of rat carotid artery had the worthy of expansion.

Abstract:目的建立健康正常BALB／c、C3H／HeJ和C3H／SW小鼠Th1／Th2中5种细胞因子正常水平，比较三种小鼠的差异性。方法用流式细胞仪及CBA试剂盒测定BALB／c、C3H／HeJ和C3H／SW小鼠血清中5种细胞因子水平。结果三种小鼠5种Th1／Th2细胞因子呈正态分布，95％可信区间可以被认可；三种小鼠IL4、IL-5水平差异性不大（P〉0．05），INF-γ、TNF-α、IL-2差异性较大，其中BALB／c小鼠Th1细胞因子水平均比两种C3H小鼠高，其中INF-γ和TNF-α具有显著性意义（P〈0．05）。结论本实验建立了健康正常BALB／c、C3H／HeJ和C3H／SW小鼠Th1／Th2中5种细胞因子的正常参考值；BALB／c小鼠比C3H／HeJ、C3H／SW小鼠处于高免疫反应状态。

Abstract:Objective To infect mouse by various paths and study the path to monitor bacterial virulence. Methods Injected mitomycin into mouse and injected Streptococcus suis type 2, EHEC O26 by wound, douching stomach, abdominal cavity. Used Enterococcus faecalis ATCC 29212 and Escherichia coil ATCC25922 as negative control. Observated clinicaling and determinated microbiolog. Results The falallty rate of the mouse that infected by douching stomach is 60 % , the fatality rate of the mouse that infected by wound is 60% . We can isolate Streptococcus suis type 2 from the dead mouse of the puncturing of heart. None of mouse that had been injected Streptococcus suis type 2 and Enterococvusfaecalis ATCC 29212 by abdominal cavity dead, hut the mouse that had been injected EHEC O26 and Escherichia coli ATCC25922 dead within 10 hotws. Conclusion In our study, we inoculated bacteria in the mouse proximal tails that scarified by artificial, then the mouse infected Streptococcus suis type 2. So, we confnrmed the path that infected Streptococcus suis type 2 by wound, we simultaneously detected that injecting Escherchia coli and other Gram negative Bacilli will cause non-specific death. It has an important significance for studying the mechanism of the pathogenicity for Streptococcus suis type 2, manufacturing and evaluating the vaccine and observing the effect of antiseptic drug.

Abstract:Objective To investigate the incidence of experimental rabbits infected with BordeteUa bronchiseptica, and then research some biological characteristics of these bacteria. Methods Bordetella bronchiseptica were isolated and identified from nasal cavities of 63 rabbits with rhinitis in four experimental rabbits fields around Nanjing, and some characteristics of the isolates including toxicity to ICR mice, pathogenicity to infant rabbits, the growth and culture characterization, antibiotics sensitivity .etc were researched. Results 19 BordeteUa bronchiseptica strains were isolated, and the rote was 30.16%. Among these bacteria, 15, 1, 3 were characterized as high, low and no pathogenicity to ICR mice, respectively. Antibiotics sensitivity tests of 5 high pathogenic bacteria strains showed these bacteria had highly susceptible to Neomycin, Chloramphenicol, Erythrocin and Tobramycin, but they were resistant to Furazolidone, Lincomycin and Streptomycin, and the sensitivity to other antibiotics was different. Conclusion Bordetella bronchiseptica was one of the important bacteria causing rabbit rhinitis. The positive rate was high, and related with seasons. These isolates were high virulence to ICR mice and pathogenicity to infant rabbits. Their characterization of culture, morphology and antibiotics sensitivity were specific.

Abstract:Objective To observe the toxic response caused by short time contact with some common disinfectants to the skin in laboratory animals. Methods Carries on the artificial skin breakage toxicity experiment using 2% glutaraldehyde, 0.2% and 2 % poracetic acid and 0.2 % sodium hypochlorite were applied to skin shaving wound in guinea pigs, and toxic changes and death of the animals were observed. Results 2 % gluaraldehyde caused erythema, edema and hemorrhagic spots. Desquamatlon occurred after 4 days and the erythema disappeared after 5 days, with a brownish discoloration left. 2% poracetic acid caused slight edema of the skin which disappoared after 3 days. 0.2 % poracetic acid and 0.2 % sodium hypochlorite did not cause any discernible skin toxic reaction. Conclusions 2 % glutarialdehyde and 2 % poracetic acid are toxic to the skin in animals and humans.

Abstract:目的建立观察蚕丝人工肌腱止点腱骨愈合及腱腱愈合的动物模型，对重建人工腱的转归进行组织学观察和评价。方法在兔胫骨中上1／3处钻孔，实验侧用经化学处理脱胶的蚕丝，一端穿骨道固定，另一端与趾长屈肌腱在轻微张力下编织缝合；对照侧以趾长屈肌腱内侧1／3的近端穿过骨道固定。分别于术后6周，12周，24周处死取标本，行HE、甲苯胺蓝及Ⅰ型和Ⅲ型胶原免疫组化染色，观察人工肌腱的腱骨愈合及腱腱愈合过程，与对照组相比评价愈合速度。结果人工腱通过胶原与骨道连接，随着时间延长，连接越广泛，并逐渐出现软骨化及骨化，有形成正常腱骨止点的趋势，但较对照组慢。结论本动物模型稳定可靠，可以作为评估人工腱腱骨愈合及腱腱愈合组织学情况的方法。

Abstract:目的利用胚胎干细胞株E14．1及新建立的C57BL／6J小鼠胚胎干细胞，采用聚合法进行嵌合体的构建。方法以E14．1及C57BL／6J干细胞为供体细胞，分为由5-10、10-15、15-20、20-25个单细胞组成的干细胞团。分别与C57BL／6J及ICR的8-16细胞期胚胎细胞聚合共培养24h再进行胚胎移植。结果E14．1与C57BL／6J胚胎的聚合及C57BL／6J与ICR胚胎的聚合均以10-15个单细胞组成的干细胞团产生的嵌合体效果最好，分别得到嵌合体小鼠11只和5只，嵌合率分别为27．50％和16．13％。结论使用聚合法成功构建嵌合体，为嵌合体的制备提供了一种简单易行的方法。

Abstract:The vaccines are considered as an useful approach for preventing the AIDS.Most of the past HIV vaccines only induced the body to product the memory B cell and antibody,but don't to prevent HIV from infecting the body.The strategy on development of vaccines against HIV basing on memory T cells could bring new hope to human vaccination.In this review,the classification,differentiation,formation and duration of memory cells,and also the possible target role for development vaccines will be presented.

Abstract:Apoptosis is a form of intrinsic cellular suicide program,which is a wide physiological process in multicellular organisms and critical for the development,the cell senescence and the tissue homeostasis.The initial and progress of apoptosis is precisely regulated.This review will summarize current knowledge of the signal transduction pathways of apoptosis.Broadly speaking,there are three pathways in apoptosis: death receptor pathway,mitochondrion pathway and endoplasmic reticulum pathway.These ways can cross-talk and regulate apoptosis coordinately.

Abstract:This paper reviewed the mechanisms of improving effects of Paliax ginseng(PG) to several stroke models.PG could improve the disorder behaviors of learning and momeory induced in different stroke models,the mechanisms related to produce a marked effect on modulating the function of cholinergic system;antiperoxidation of free radical;inhibit the apoptosis of neurocyte;protecting the function of chondrisome;relieving the neurotoxicity of excitatory amino acid; regulating the activity of NO.

Abstract:实验动物设施集中空调通风系统存在嗜肺军团菌、β-溶血性链球菌、真菌等致病微生物污染的可能，直接影响到实验动物的质量和工作人员的健康，并带来严重的公共卫生安全问题，应该在新建、改建、扩建和运行阶段实施预防空气传播性疾病的卫生学评价与公共卫生安全管理制度建设。