Abstract:目的为研究2型糖尿病机理和糖尿病药物提供动物模型和基础资料,探讨建立2型糖尿病地鼠模型的方法并观察模型的稳定性。方法采用高能量高脂饮食结合腹腔注射小剂量链脲佐菌素(STZ)的方法建立2型糖尿病地鼠模型,并监测不同时间点空腹血糖、血脂、体重、胰岛素等指标,进行统计分析。结果地鼠注射STZ后第2天大部分血糖值达到成模标准,并逐渐出现不同程度糖尿病的表现,其中雄鼠约在给药第14天,雌鼠约在给药第6天,血糖达到稳定,观察至第14周,均满足成模标准。结论高能量高脂饮食结合小剂量STZ腹腔注射建立2型糖尿病地鼠模型的方法,是目前制备2型糖尿病动物模型的较好方法。其稳定成模时间与性别有关,雌鼠稳定成模时间较雄鼠早,而成模率及模型稳定性与性别无关。

Abstract:Objective To investigate the relationship between single nucleotide polymorphism(SNP) C1330T of syndecan-3(SDC3) gene and hypercholesterolemia,lipid profile in a Chinese rural population.Methods Hypercholesterolemia(HTC) was defined as serum total cholesterol equal to or more than 200 mg/dL.Genotyping for C1330T was completed by using PCR,restrictive enzyme digestion,and sequencing in 336 males(HTC 161,normal total cholesterol(NTC) 175),1491 females(HTC 935, NTC 556).Serum total cholesterol(TC),HDL-cholesterol(HDL-C),LDL-cholesterol(LDL-C),triglyceride(TG),glucose(Glu) level were measured.Results The significant difference of distribution of C1330T was observed between HTC and NTC in males.1330C was associated with HTC in men(odds ratios(OR)=1.42,95% confidence intervals(CI)=1.040-1.926,P< 0.03),but not in women(OR=1.06,95%CI=0.92-1.24,P=0.41). After adjusted by age,body mass index(BMI) and other conventional risk factors,the association remained in men.For 1330C,there was a borderline statistically significant allele*gender interaction(P=0.0742).The association between C1330T and serum HDL-C,LDL-C level were observed in men.Conclusion The distribution of C1330T of SDC3 gene was associated with hypercholesterolemia,lipid profile in men.

Abstract:目的本研究观察高脂餐诱导的兔AS模型中,COX-2在AS发生和早期病变中的表达,以及在此过程中增生内膜降解基质的能力、胶原含量、内弹力板的变化;以及DFU[5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulphonyl)-phenyl-2-(5H)-furanone]-特异性COX-2抑制剂的作用。方法42只6周龄雄性新西兰大白兔(NZW),饲以高脂餐(2%胆固醇 3%猪油),参考血脂反应程度,随机分为四组,在高脂餐的同时,分别管饲安慰剂与不同剂量的DFU。4周后取主动脉(主动脉根部-髂动脉分叉)。主动脉弓前1/3切片,行HE染色;后2/3匀浆,测明胶酶活性及免疫印迹检测MMP-2。其余主动脉标本纵向剖开,以4%中性甲醛固定,苏丹Ⅳ染色,内膜拍照,Matlab软件分析粥样硬化斑块面积占内膜总面积比例。结果主动脉内膜COX-2随AS的进展表达逐渐增高。DFU对各组体重、血浆总胆固醇与甘油三酯无显著性影响。不同剂量DFU组AS面积比例分别为:对照组,0.330±0.052(n=7);实验1组,0.273±0.042(n=6);实验2组,0.314±0.089(n=8);实验3组,0.288±0.036(n=9)。与对照相比,DFU对实验1、23、组主动脉AS面积没有明显影响(P>0.50)。DFU对病变血管内MMP-2的表达和活性亦没有影响。结论COX-2与AS发生和进展相关,但未发现特异性COX-2抑制剂DFU对AS进展的抑制作用。

Abstract:Objective To analyze the genetic polymorphism of 14 inbred mice in 24 microsatellite loci and to establish a method for detecting the genetic quality of inbred mice by using PCR technique.Methods We selected suitable microsatellite loci and primers according to the database of Mouse Genome Informatics to amplify DNA in 14 inbred strains of mice,and analyzed the polymorphism of 14 strains of mice in these 24 microsatellite loci.Result There were remarkable polymorphism in 24 microsatellite loci among 14 inbred strains or sub-strains.The genetic distance between strains was from 0.045 to 1.526.Conclusion The microsatellite markers can be used for distinguishing different mice strains and sub-strains.So we should use microsatellite markers to monitor the genetic quality of the inbred mice.

Abstract:Objective To discuss achievement ratio and mortality rate of preparation of porcine model of biliary cirrhosis,and seek methods to evaluate hepatic function reserve.Methods Choosing ten common pigs as experimental animal,cutting the belly open,dissociating and ligating common bile duct, during the course of the experimental procedure,we collect samples and measure portal venous pressure regularly.Results The rate of success is 100 percent and the rate of mortality is 40 per cent.ALT and AST go up step by step in the early stages,then go steady.TBIL increases continuously,on the contrary,albumin decreases continuously.Conclusion Rate of success of model of hepatic cirrhosis is more higher and time which is needed is shorter,but the rate of mortality is more higher.Pathology biopsy is still a gold standard of diagnosing fibrosis at present.The changes of ALT and AST have no significance for evaluating the development of hepatic cirrhosis.Albumin and bilirubin have obviously difference in before-and after-liver cirrhosis,which is useful to evaluate liver cirrhosis,but sensibility is poor,we should take active in seeking more valuable indexes.

Abstract:Objective To study the effects of the Lipid Metabolism and Anti-oxidation on PCOS rats.Method PCOS models were established using rats.The kind of animal model was used to observe body weight,estrous cycle,Lipid Metabolism,Leptin,SOD and MDA.Results Lipid Metabolism,leptin level and MDA was significantly higher in PCOS model group.Conclusion PCOS can cause dyslipidemia in rats,reducing the role of oxygen free-radical oxidation.

Abstract:Objective To investigate the pattern feeding nutritional standard and breeding management of model animals.Methods In order to provide scientific method for oncology,the human liver cancer model was established by transplanted BALA/c nude mouse(nu/nu),and the growth was controlled by enhancing nutritional component and strength the feeding management.Results By feeding up and enhancing,the tumor growth of model animals was controlled gradually and survival constitution of animal was improved remarkedbly,and the bearing tumor survival time was delivered obviously.Conclusion The protein nutrition of feed could impact on survival constitution and effect of animal experimental,this have the effective significantly for establishment of other animal method.

Abstract:Objective To explore the relationship between vascular calcification and hyperlipidmia and interfered with simvastatin.Method Thirty-six male SD rats were divided into six groups randomly: control group;vascular calcifid group;hyperlipidmia group;vascular calclfid group based on high lipid diet;vascular calcifid group therapid with simvastatin;vascular calcifid group based on high lipid diet tharopid with simvastatin.At the end of six weeks of drug administration,all rats were sacrificed.Total cholesterol and highdensity lipoprotein levels in serum and alkaline phosphate activity and total calcium content in aorta were measured and calcium deposit were observed by Von Kossa stain.Results With 288.89% and 259% increase in the total Ca~(2 ) content and ALP activity in aorta in vascular calcifid group than control group.With 520.11% increase in the total cholesterol and 22% decrease in the high-density lipoprotein cholesterol in serum in hyperlipidmia group than control group.With 36.49%,and 21.94% increase in the total Ca~(2 ) content and ALP activity in aorta,and 571.56% increase TC,30% decrease HDL in serum in vascular calclfid group based on high lipid diet than vascular calcifid group.Conclusion Simvastatin can alleviate vascular calcification,hyperlipidmia and vascular calcification are causes and effects each other.Simvastatin can postphone vascular calcification and reduce lipoprotein in serum at the same time.

Abstract:目的了解喜爱迪20和灭菌可灵用于实验动物生产环境喷雾消毒和器具擦拭消毒的效果。方法采用营养琼脂培养皿表面接种的方法,在环境和器具消毒前后对两种消毒剂的消毒效果进行了检测。结果喜爱迪20用于喷雾消毒时,对房顶、左右两侧墙壁、走廊等位置的灭菌效果非常好,消毒前后细菌的减少率在88.2%~100%之间,各个位置消毒后第3天和第5天的细菌数则差别较大。灭菌可灵的擦拭消毒的结果显示对笼架和地面的短时间消毒效果非常好,细菌的减少率在99.22%~100%之间,但消毒效果的保持情况不是非常理想。结论喜爱迪20和灭菌可灵可以用于实验动物环境和器具的消毒。

Abstract:目的探讨硒酸精氨酸对小鼠酒精性肝损伤的保护作用。方法用连续灌服酒精的方法建立小鼠酒精性肝损伤模型。将60只雄性小鼠随机分为四组:对照组,模型组,硒酸精氨酸处理组和二氧化硒处理组。连续灌胃8周后,测定小鼠血清中天冬氨酸氨基转移酶(AST)、丙氨酸转移酶(ALT),肝匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽(GSH)的含量。对肝脏切片做HE染色和Masson染色,在光镜下观察肝脏病理学变化。结果硒酸精氨酸能显著降低酒精性肝损伤引起的AST的升高(P<0.01),有效保持了肝脏中GPx和SOD的活性(P<0.01)。病理学观察结果与酶学变化相一致,硒酸精氨酸对肝脏的保护作用明显优于二氧化硒。结论硒酸精氨酸对小鼠酒精性肝损伤具有显著的保护作用,效果也优于二氧化硒。

Abstract:目的对近交系Wistar大鼠进行繁殖性能的测定。方法选取血缘扩大群共18对,90日龄开始按1♀*1♂进行交配,统计其生长繁殖性能。结果第二、三、四胎的平均窝产仔数和断奶窝重比第一和第五胎高。第一胎仔鼠从出生第3天到第7天(增重9.42 g)、第14天到第21天(13.98 g)快速生长。母鼠怀孕期、哺乳期体重、饲料和水的日消耗量有明显的差异,母鼠哺乳期饲料和水的日消耗量均比怀孕期高,母鼠怀孕期体重明显比哺乳期重,母鼠怀孕期体重后期比初期增加150 g左右,曲线呈上升趋势。结论本群Wistar近交系大鼠的生长繁殖符合近交系大鼠的生长繁殖规律。

Abstract:Objective To investigate the development and kinesis of NOS isoforms in different reperfusion time in a model of reversible middle cerebral artery occlusion(MCAO) in rats.Methods Male Sprague-Dawley rats were anesthetized with isoflurane and subjected to an intraluminal MCAO for 90 minutes and reperfusion thereafter.The activity of the different isoforms of NOS(cNOS and iNOS) from the right and left hemispheres was measured at 1,2,6,9,12,and 24 hours reperfusion respectively.Following neurological deficit evaluation,brains were removed and sectioned at different reperfusion time point respectively.The infarct volume was determined with an imaging analysis on the brain sections stained with TTC.Results Conditions of severe MCAO induced ischemia and reperfusion excessively activate NOS,resulting in activities of cNOS and iNOS were significantly increased in the ischemic hemisphere at all time points of reperfusion for 24 h. The peak activity of cNOS was at 6h after reperfusion while that of iNOS appeared at 9 h.The infarct volume was increased parallel to the increasing of NOS.Conclusion cNOS from endothelial cells(eNOS) and neurons(nNOS) is constitutively expressed enzymes,whose activity is stimulated by early ischemic and reperfusion injury and get peak activity in the brain at 6 h after reperfusion iNOS,which is very little present in healthy tissue,is induced shortly after ischemia but its peak activity appeared at 9 hours after reperfusion.Both cNOS and iNOS contribute to secondary late-phase ischemic damage.

Abstract:Objective To design the barrier system of containing animal husbandry and animal experiment according to the principle of efficient, safety, efficiency and utility. Method The combined transportation unit, life unit, bufferl unit and experimental unit were mainly designed in this barrier instrument. During the process of the animal and goods transfer, there is a automatically controlled sterilizing system. Result The design improved work efficiency and guaranteed effectively cleaning degree of laboratory animal. Conclusion Results shows that the multiplex barrier system will be aviable not only for animal production but also for animal experiment in miniature laboratory animal facihies of SPF and clean rodents.

Abstract:Mitogen-activated protein kinases,such as ERK,JNK,and p38,regulate the cell growth,differentiation,apoptosis and cell-cell interaction responding to growth factors,agonists and stress stimuli.Mitogen-activated protein kinases play an important role in the process of the cardiomyopathy and heart failure.Mitogen-activated protein kinase phosphatase-1 is a shock protein to dephosphorylate the mitogen-activated protein kinases.It inhibits the activity of mitogen-activated protein kinases,which is activated by stress stimuli and plays role in the process of heart failure.In this review paper,the roles of mitogen-activated protein kinases and mitogen-activated protein kinase phosphatase-1 were summarized from the in vivo research results of transgenic animal models.

Abstract:Dilated cardiomyopathy(DCM) is a myocardial disorder characterized by cardiac dilatation and contractile dysfunction of the left and/or right ventricles,which is one of the cause of heart failure in addition to coronary heart disease and hypertention.Familial DCM may account for 35% of DCM. The mutations of sarcomere filament protein and cytoskeletal protein were largely found in familial DCM,and the mutations in mitochondrial DNA were also found in familial DCM.In this review,we focus on the molecular genetics of familial DCM.

Abstract:Gene defects may account for 50% of hypertrophic cardiomyopathy(HCM) and 35% of dilated cardiomyopathy(DCM).Pathophysiological mechanisms that implicated in HCM and DCM include defective force generation,due to mutations in sarcomeric protein genes;defective force transmission,due to mutations in cytoskeletal protein genes.Cardiac troponin C and cardiac troponin I are linked to actin and tropomyosin by cardiac troponin T,which play an important role in cardiac contractility and relaxation.Many cardiac troponin T gene mutations have been found in HCM and DCM,and study of cardiac troponin T gene mutations may help to elucidate the pathogenesis of cardiomyopathy.In this review,we focus on cardiac troponin T gene mutations in cardiomyopathy.