Abstract:目的 筛选表达荧光素酶(luciferase)基因的人单克隆肝癌细胞系,用活体成像技术(bioluminescent imaging)在细胞及整体动物水平检测其发光能力,为监测肿瘤生长和转移建立一种新的肿瘤动物模型.方法 构建表达荧光素酶基因的真核表达载体并将其转入肝癌细胞(BEL-7405),用G418筛选稳定表达荧光素酶的单细胞克隆,在体外用活体成像技术评价其稳定发光能力.BALB/c nu/nu裸鼠皮下接种1×106个发光细胞使其成瘤,活体内观察肿瘤的生长及转移情况.结果 在肝癌细胞中获得稳定表达荧光素酶的克隆,利用活体成像技术可检测到细胞克隆发光.将稳定表达荧光素酶的克隆皮下接种到裸鼠体内可成瘤,利用活体成像系统观察了肿瘤的生长过程,肿瘤发光随着观察时间的延长而增强,但是没有观察到肿瘤转移的现象.结论 本研究筛选得到了稳定表达荧光素酶的肿瘤单克隆细胞系,结合活体成像技术,建立了一套新的能够用于活体内监测肿瘤生长的研究方法.

Abstract:目的建立大鼠IgA肾病（IgAN）模型，测定大鼠血清中的白介素-6（IL-6）、纤维结合蛋白（FN）、一氧化氮（NO），探讨这些指标水平的变化与IgAN免疫损伤的相关性，为临床治疗提供动物实验研究依据。方法24只大鼠被随机分成3组，每组8只。模型组和治疗组用免疫复合物法复制；正常对照组用生理盐水。10周后，治疗组大鼠被给予雷公藤多甙片3周。留取所有大鼠血清测定IL-6、FN、NO；留取所有大鼠尿液测定红细胞（RBC）、总蛋白量（TPR）；留取所有大鼠肾组织作病理学检查。结果模型组中大鼠尿液中RBC、TPR含量较治疗组及正常对照组显著增高（P〈0．01）；血清中的IL-6、FN的水平较治疗组及正常对照组显著增高（P〈0．01）；血清中的NO水平较治疗组及正常对照组显著降低（P〈0．01）。治疗组的大鼠肾组织病理损伤程度较模型组明显减轻（P〈0．01）。结论血清中的IL-6、FN及NO的水平与RBC数、TPR及肾组织病理损伤程度相关，它们可作为观察IgAN治疗效果的重要指标，也可作为IgAN严重性的预测指标。下调血中的IL-6、FN及上调NO的水平，可减少IgA与FN免疫复合物的形成，从而改善肾组织的免疫损伤。

Abstract:Animal facilities are vulnerable to hurricanes and other natural disasters, which can endanger lives and disrupt critical animal care routines. Facility managers must therefore prepare a rigorous emergency plan that ensures human safety while considering the specific needs of all animals on site. The author presents guidelines and recommendations for disaster preparedness based on her experience at a facility in Florida, where hurricanes are relatively common. An effective plan must include a priority system, a well-trained emergency response team, efficient communication methods and concrete provisions for animals and employees. Lab Animal 2007,36（10）

Abstract:目的评估组织工程血管补片动物模型的可行性并总结模型建立的围手术期处理。方法10只成年杂种犬，用自身的骨髓细胞和高分子可降解材料构建的组织工程血管补片扩大肺动脉流出道。结果实验中有1例在术中出现心动过缓，暂停手术操作后，其余手术均逐渐恢复，未造成不良后果。术后实验动物均存活。术后5～10min撤离呼吸机，拔除胸引管。术后两周肺动脉造影示左肺动脉通畅，未见动脉瘤形成，移植物处稍狭窄；取出移植物观察，血管管腔通畅，腔面光滑，无血栓，无感染。结论通过自身的骨髓细胞和高分子可降解材料构建的组织工程血管补片扩大犬肺动脉流出道成功建立了组织工程血管补片动物模型。为了保证模型的成功建立，应使用右侧卧位、尽早进行心电监护、及时处理心律紊乱和尽早撤离呼吸机等围术期护理要点。

Abstract:目的比较雄性Fmr1基因敲除小鼠和FVB小鼠血液生理生化值和血清性激素水平，探讨Fmr1基因对动物生长发育和生殖生理等方面的影响。方法分别测定血液生理指标、血清生化指标、血清电解质和血清E2、LH、FSH、T和PRL的含量，并进行统计学处理和分析。结果雄性Fmr1基因敲除小鼠与FVB小鼠比较，血液生理指标中MCV和PCT有显著差异（P〈0．05），而RBC、HCT、HGB、MCH和WBC等无显著差异（P〉0．05）；血清生化指标中除TBIL、[IP^3＋]、[Mg^2＋]（P〈0．05）和AIP、BUN（P〈0．01）外，TPROT、GLB、A／G、BUN、CREAT、[K^＋]、[Na^＋]等项均无显著差异（P〉0．05）。性激素水平E2、LH值差异无显著性（P〉0．05），FSH、T、PRL差异极显著（P〈0．01）。结论Fmr1基因可影响动物的某些生理生化及激素水平。

Abstract:目的探讨外源性重组蟹金属硫蛋白（rMT）对长爪沙鼠脑缺血再灌注损伤的抗氧化作用。方法160只长爪沙鼠随机分为4组（每组又分为4个亚组），比较假手术组（Sham）、脑缺血再灌注组（I／R组）、MT预处理组（MT组）和依达拉奉阳性对照组（ED组）分别再灌注2d、3d、5d和7d后，血清的谷胱甘肽（GSH）和丙二醛（MDA）含量及谷胱甘肽过氧化物酶（GSH—Px）、超氧化物歧化酶（SOD）活性和总抗氧化能力（T—AOC）的变化。结果MT组在一定的时间点能明显提高血清中GSH—Px、SOD和T-AOC的活性（P〈0．05），减少MDA含量（P〈0．05）。结论MT在一定的时间窗内可增强体内抗氧化酶活力，阻止脂质过氧化水平的升高，减轻脑缺血再灌注引起的神经元损伤，但MT在缺血再灌注的早期对GSH含量的作用不明显。

Abstract:目的研究兔双侧颈总动脉结扎前后脑血管的改变情况。方法经股动脉插管行兔脑血管造影，再行双侧颈总动脉结扎；15d后行第二次脑血管造影，并进行前后对比。结果与结扎前比，结扎后大部分兔脑血管，尤其是基底动脉迂曲、延长、增粗。颅内外侧支循环广泛形成。结论颈总动脉结扎后，兔脑可能靠侧支循环增加和基底动脉增粗来代偿脑血流的下降；在症状性脑血管痉挛模型的制作中，颈总动脉结扎是一项可行的技术。

Abstract:Objective To determine the effect of two type of demineralization solutions on the production of artificial carious lesion in bovine enamel and human enamel in vitro. Methods A partially saturated acidic buffer solution and an acidifed hydroxyethylcellulose gel system were used as decalcifying media for the preparation of initial artificial carious lesion in bovine enamel and human enamel. After demineralization, these enamel specimens was observed by microscope and scaning electron microscope and compared in histological pathology. Results The results indicated all specimens in a partially saturated acidic buffer solution could morphologically form subsurface demineralization, which were the same as the early smooth enamel caries. Lesions in bovine teeth were pathologically similar to those in human teeth, except that the lesions depth was deeper than those in human teeth. However, In gel, regular subsurface lesions were hardly produced in all specimens. They were either eroded or dissolved. Conclusion It was concluded that caries-like lesions could be consistently produced in bovine enamel with a partially saturated acidic buffer solution. So this demineralization system can be use for reproducible preparation of artificial carious lesions in dental enamel.

Abstract:At the 2006 National Meeting of the American Association of Laboratory Animal Science, a panel discussed the question of what constitutes optimal or acceptable housing density for mice. Though there is a consensus that present guidelines are somewhat arbitrarily defined, scientific research has not yet been able to provide clear recommendations for amending them. Speakers explored the many factors that influence decisions on mouse housing, including regulatory requirements, scientific data and their interpretation, financial considerations and ethical concems. The panel largely agreed that animal well-being should be the measure of interest in evaluating housing density and that well-being includes not only physical health, but also animals＇ behavior, productivity and preference. Lab Animal 2007,36（10）

Abstract:目的应用5-HT抗血清研究40周HBK-SPF鸭胃肠道内的5-HT免疫活性细胞的分布密度及形态，并探讨其分布型的成因及细胞形态与功能的关系。方法免疫组织化学ABC法。结果5-HT细胞主要分布于十二指肠及其以下部位，腺胃偶见，分布密度近似呈波浪形，其中以直肠分布密度最高，小肠呈U形分布。5-HT细胞的形态多样，呈圆形、椭圆形、锥体形等，主要分布于胃肠粘膜上皮之间、上皮基部、固有膜以及腺泡上皮之间等。结论40周HBK—SPF鸭5-HT细胞分布型的形成与各部位消化功能有关；根据其形态，我们认为40周HBK—SPF鸭胃肠道内5-HT细胞具有内、外分泌两种功能。

Abstract:目的 探讨乙醚吸入与戊巴比妥钠联合麻醉应用效果.方法 随机将20只受肝大鼠分成两组,每组10只.A组为单纯的乙醚吸入麻醉对照组,B组先用乙醚吸入作麻醉诱导,再腹腔注射2%戊巴比妥钠作麻醉维持.观察手术时间、术中追加麻醉次数、术后苏醒时间和死亡率.结果 与B组相比,A组手术时间明显延长(P＜0.05),术中平均需要重复吸入乙醚5±1.63次,以维持麻醉状态.B组术中麻醉平稳,不需要继续给药,两组术后苏醒时间无显著性差异(P＞ 0.05).结论 乙醚吸入与戊巴比妥钠联合麻醉,可延长麻醉的有效时间,加强麻醉效果,有效地保证手术的顺利进行.

Abstract:目的测定转基因C57BL／6-Tg（ACTB—EGFP）1osb／J（EGFP）小鼠主要脏器重量和脏器系数。方法实验选用5～6周雄性、6～7周雌性小鼠各15只，用sartorius电子天平分别测定体重和9个主要脏器重量，计算脏器系数，并对雌雄脏器重量和脏器系数之间进行比较。结果雌雄小鼠脏器重量间比较，雄性鼠体重明显大于雌性的体重（P〈0．01）；心、肝、肺、肾、肾上腺的重量间差异极显著（P〈0．01）；脾脏比较差异显著（P〈0．05）；雌雄间脏器系数比较，肺、脑、肾上腺间差异极显著（P〈0．01），心、肝、脾、肾脏间差异不显著（P〉0．05）。结论转基因EGFP小鼠不同性别间脏器重量及脏器系数间有一定的差异，为相关研究打下了基础。

Abstract:Objective To observe relationship between some serum index, such as T-BIL, ALT, AST, TP, ALB, and gender as well as age in C57BL/6J-HBV transgenic mice.The differences between HBV mice and normal C57BL/6J mice in blood serum also determined. Methods Eight-week, twenty-four-week C57BL/6J-HBV transgenic mice and normal C57BL/6J were put into experiments, T-BIL, ALT, AST,TP and ALB of blood serum were determined. Results There was significant difference of T- BIL,ALT,AST, TP and ALB between HBV mice and C57BL/6J mice. The great differences of ALT and AST value were also observed in different age HBV mice. Conclusion The value of T-BIL,ALT,AST,TP and ALB is remarkable higher in HBV mice than that in C57BL/6J.The value of ALT and AST is related to the age of HBV mice and unrelated to the gender.

Abstract:目的 评价东莨菪碱在不使用呼吸机家猪基础麻醉中对心率、血压的影响.方法 与结果 48头家猪分为东莨菪碱组和山莨菪碱(654-Ⅱ)组,基础麻醉后两组心率、收缩压、舒张压分别为532.08±105.67 ms与394.17±59.78 ms, 157.00±18.26 mmHg与178.63±20.73 mmHg, 118.63±16.51 mmHg与128.21±17.00 mmHg,两组比较有统计学差异.结论 东莨菪碱作为家猪的基础麻醉用药,对心率血压的影响较654-Ⅱ小.

Abstract:目的探讨红鲫实验动物的营养需要、科学配制红鲫饲料。方法用常规方法测定红鲫幼鱼、红鲫成鱼的含肉率及肌肉营养成分。结果红鲫幼鱼、红鲫成鱼的含肉率为46．34％、42．35％；红鲫幼鱼、红鲫成鱼肌肉（鲜样）中分别含水分79．11％、78．49％，粗蛋白质12．47％、14．72％，粗脂肪6．95％、4．37％，粗灰分1．01％、1．41％，无氮浸出物0．47％、1．07％，能量5．65kJ／g、5．24kJ／g，其E／P值（能量蛋白比）为45．32kJ／g、35．69kJ／g。结论红鲫幼鱼、红鲫成鱼含肉率、肌肉营养成分有一定差异。

Abstract:Objective To observe the changes of biochemistry indexes in SD rats after anesthesia. Methods 50 SPF SD rats aged 2 months, 28 males and 22 females were collected blood in 0,5,10,20min after anesthesia, and serum ALT,AST,GLU, BUN ,TP,ALB ,AKP, CREA, CHOL, TG were detected after fasting of 12 hours. Results The biochemistry indexes in SD rats varied as anaesthetic time increases. Conclusion Standaring the blood collecting operation, and reducing the time to wait for collecting blood in postanesthesia SD rats can decrease the changes of biochemistry indexes.

Abstract:目的 根据不溶性钙盐离子积计算,将血钙控制于正常水平,从而将血磷酸与氟控制在致死性浓度以下,达到解救磷与氢氟酸烧伤中毒目的.方法 氢氟酸与黄磷于新西兰兔背部皮肤致致死性磷与氢氟酸烧伤,伤后动物分为氢氟酸烧伤组(HF,n=10);氢氟酸烧伤钙治疗组(HF-Ca,n=10);磷烧伤组(P,n=12)与磷烧伤钙治疗组(P-Ca,n=12),汽油烧伤对照组(G,n=12).两治疗组均给予钙治疗以将血钙水平控制在正常水平以上,观测伤后死亡率与血氟、磷、血总钙、游离钙水平.结果 氢氟酸烧伤组伤后血氟(1.15±0.12)×10-4 mol/L, 动物死亡率100%;磷烧伤后血磷显著升高,动物死亡率75%;两组血游离钙均较正常显著降低(P ＜0.05),钙治疗将血钙维持于正常水平后,7 d内动物死亡率降低至普通烧伤的30%水平.结论 维持血钙于正常水平能够防治磷及氢氟酸烧伤致死性中毒,这一结果能够从不溶性盐离子积计算中加以解释.

Abstract:目的建立仙台病毒的RT—PCR方法并应用于常规检测。方法根据仙台病毒（gi：9627219）F蛋白的基因序列，设计Ff和Fr引物，以仙台病毒总RNA逆转录的cDNA为模板扩增出约609bp预期条带；将建立的RT—PCR方法应用于人工感染小鼠和送检细胞样品检测。结果八份感染小鼠样品中全部扩增出约609bp目的条带，16份肿瘤细胞样品有12份扩增出609bp目的条带。结论建立的仙台病毒RT—PCR方法是一种灵敏、快速、特异的方法，能够用于常规检测。

Abstract:目的 探讨不同麻醉方法建立大鼠肺鳞癌模型的差异.方法 体重180～220 g Wistar大鼠,依不同麻醉方法分为2组实验I组(110只)用0.3%戊巴比妥钠麻醉;实验Ⅱ组(80只) 基础麻醉用盐酸氯胺酮44 mg/kg,然后乙醚吸入麻醉.两组均采用额镜直视法,灌注3-甲基胆蒽(MCA)、二乙基亚硝胺(DEN)与普通碘油混悬液于大鼠左下叶支气管.结果 麻醉显效时间和维持时间分别为Ⅰ组,(23.50±1.98) min,(246.56±15.46) min,Ⅱ组,(3.05±0.45) min,(12.47±1.35) min,两组间有显著性差异(P＜0.01);灌注成功率、存活率、诱癌率分别为Ⅰ组94.55%,48.08%,90.00%,Ⅱ组93.75%,90.67 %,92.65%,两组成功率、诱癌率差异无显著性(P ＞ 0.05),存活率有显著差异(P ＜0.01).分别于不同时间处死大鼠,2组基本病理过程相同.结论 戊巴比妥钠麻醉剂量易控制,以小剂量灌注雄性大鼠为佳;盐酸氯胺酮与乙醚复合麻醉较难控制,但速度快,实用性强.

Abstract:Scientific research has yet of cage size on mouse breeding to conclusively determine the optimal cage size for mice. The authors examined the effect performance and on offspring behavior, which can serve as indications of overall wellbeing. They housed breeding trios of C57BL/6Tac mice in standard or large individually ventilated cages and measured four reproductive parameters： litter size; litter survival to weaning age; average pup weight at 7, 14 and 21 days; and the number of days between litter births. They investigated the behavior of a subset of male and female pups from parents housed in cages of each size in the elevated plus maze test, the open field assay and the acoustic startle test. Cage size had no significant effect on any of the reproductive parameters measured and few or inconsistent effects on behavior in weaned pups. Lab Animal 2007,36（10）

Abstract:Animal model of human disease plays an important role in medical research, but spontaneous human disease cannot satisfy needs of medical research. Thus, it has wide applied prospect that research of animal model of human disease is performed by using gene targeting. Gene targeting technology and its application in research of animal model of human disease were briefed in this paper.

Abstract:Reproductive Cloning is a technique which produces offspring with the same genetic background by cellar proliferation other than bisexual mating. It has tremendous value for saving extinguishing animal. This review summarized several methods of reproductive Cloning, and then focused on nuclear transplantation and factors that affect the efficiency of nuclear transplantation.

Abstract:在实践经验的基础上，本文对实验动物屏障设施运行管理中的软件建设、内环境的洁净维持、配套设备的管理、“四流”的净化控制等几个关键问题作了论述，希望能对我国实验动物屏障设施管理水平的提高有所帮助。

Abstract:克罗恩病和溃疡性结肠炎统称为炎症性肠病（IBD），病因虽不明确，但对其发病机理已有了较多的了解。该病的发生是由于个体易感性、肠道菌群和粘膜免疫相互作用所致。炎症性肠病动物模型可通过化学性诱导、免疫学、遗传学等方法获得。三硝基苯磺酸与乙醇灌肠致炎法是目前最常用的方法，本文侧重概述介绍三硝基苯磺酸诱导的炎症性肠病的机制、模型、应用及优缺点，为疾病的研究、治疗和新药的开发提供指导。