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Abstract:At present the development of mammalian nuclear transfer and transgenic animal is very quickly.In 1997,'Dolly' was successfully cloned from adult cell,and it was a landmark development of clone.This review describes the recently developed animal clone techniques,problem and research mechanism.Every aspects are also discussed,as well as potentials of using clone method to develop transgenic animals.

Abstract:目的甲型H1N1流感病毒A/California/7/2009与A/California/4/2009病毒序列比较同源性在99%以上,本实验旨在比较两株病毒感染BALB/c小鼠研究感染力强弱。方法分别将A/California/7/2009（CA7）与A/California/4/2009（CA4）两株病毒分别连续10倍稀释后,对4~6周龄雌性BALB/c小鼠经乙醚麻醉后进行滴鼻攻毒,每个稀释度接种10只实验小鼠,测定CA7 MLD50为101.24/0.05 mL,检测小鼠感染、致病的多项指标,观察期为14 d。结果相同TCID50的CA7和CA4病毒感染小鼠,CA4感染小鼠后14 d内死亡率为20%,而CA7感染小鼠后8 d内死亡率为100%。CA7 106TCID50感染的小鼠病理表现为重度弥漫性间质性肺炎,CA4 106TCID50感染的小鼠病理表现为中度-重度间质性肺炎。结论在相同条件下,CA7感染力明显强于CA4。

Abstract:Objective To investigate the effect of Morroniside which was isolated from Cornus of ficinalis on recovery of neurological function after focal cerebral ischemia reperfusion in rats.Methods Wistar male rats were randomly divided into sham operated group,model group and Morroniside-low(30 mg/kg),Morroniside-middle(90 mg/kg),Morroniside-high(270 mg/kg),vitamin E(35 mg/kg).Focal stroke was induced in adult rats by 30 minutes right middle cerebral artery occlusion reperfusion injury(tMCAO/R).To examine the reco...

Abstract:目的建立稳定表达绿色荧光蛋白的人宫颈癌细胞系,建立移植瘤模型并比较移植模型肿瘤生长的荧光分析和卡尺测量的优缺点。方法以Lipofectamine 2000介导chickenβ-actin-GFP-NEO转染人宫颈癌细胞Hela,经梯度浓度G418筛选获得稳定表达绿色荧光蛋白的细胞克隆并扩大培养。BALB/cA-nu裸鼠皮下接种1×10^6个发光细胞使其成瘤,利用活体荧光成像系统和游标卡尺观察肿瘤的生长情况。结果获得了稳定表达GFP的人宫颈癌细胞株,将其接种到裸鼠体内可成瘤。活体荧光成像观察发现,1至3周随着肿瘤体积逐渐增大,平均荧光光子数逐渐增加;4周时随着肿瘤出现明显坏死,平均荧光光子数呈现下降趋势,而游标卡尺测量结果显示肿瘤在4至5周仍然不断的增大。结论绿色荧光蛋白能够在人宫颈癌细胞Hela中长期稳定表达,用绿色荧光蛋白标记的人宫颈癌细胞Hela建立的裸鼠肿瘤模型可以为人宫颈癌研究提供理想的实验材料,应用小动物活体成像系统能够客观定量评价活的肿瘤细胞在动物体内的生长情况,而不是肿瘤体积的变化。

Abstract:Objective To develop a proper animal models representing human diabetes,the efficiency and doses of alloxan in male and female animals was investigated.Methods Alloxan were administered to beagle dogs and mice with different dosage.Blood glucose was tested after treatment at the 3rd,7th,14th,and 21th day,respectively.Results The different effects of alloxan on the blood glucose concentration was showed in female and male animals.After identical doses of alloxan injected,the blood glucose concentration in fe...

Abstract:Objective To compare the effect of berberine and additive on serum glucose and lipid level.MethodsType 2 diabetic model was induced by both high lipid food and low dose of streptozotocin(STZ) in mice.The number of 100 three month's mice were randomly divided into normal group,type 2 diabetic model group,berberine treated group,berberine and low dose additive treated group,berberine and high dose additive treated group and metformin treated group.Every group were administered through intragastric administrat...

Abstract:Objective To generate the CTF1 gene transgenic mice specifically expressed in nervous tissues and to make an animal model for the study of its function and effects on Alzheimer's disease.Methods Transgenic mice were created by the methods of microinjection.The genotype of transgenic lines was identified by PCR.The brain specific expression of the CTF1 gene was analyzed by Western Blot.The histological changes of the brain from the transgenic mice were analyzed by microscope observation.Results Two transgeni...

Abstract:目的建立系统表达肝素结合性表皮生长因子（HB-EGF）的转基因动物模型,利用转基因动物模型研究HB-EGF与组织纤维化的关系。方法RT-PCR法克隆小鼠HB-EGF基因,将其插入Chickenβ-actin启动子下游,构建Chickenβ-actin-HB-EGF表达载体,利用显微注射的技术把表达载体注射到受精卵的雄原核中,建立HB-EGF转基因小鼠。利用特异引物PCR的方法鉴定转基因的基因型,采用Western Blot方法鉴定HB-EGF基因在全身组织的表达。分别取HB-EGF转基因鼠与同窝阴性小鼠的肝、肾、肺及膀胱组织进行Massion染色。结果建立了系统表达HB-EGF转基因小鼠,Western Blot发现其HB-EGF在肝、肺、肾及膀胱的表达与同窝阴性对照小鼠相比明显增加。Massion染色结果表明转基因鼠肝、肺、肾及膀胱组织纤维化程度明显高于同窝阴性对照小鼠。结论成功建立了系统表达HB-EGF转基因小鼠,HB-EGF的过度表可显著加重组织纤维化程度。

Abstract:Objective To establish the transgenic mouse of 24-dehydrocholesterol reductase gene(Dhcr24) to study the effect of Dhcr24 on metabolism in vivo.Method\ The mouse Dhcr24 was clone by RT-PCR and the sequence of the cDNA was confirmed by sequence analysis.The transgenic plasmid was constructed by inserting the Dhcr24 gene in the downstream of CMV promoter.The transgenic mice were generated by microinjection method and the genotype was detected by PCR.The expression levels of the gene were determined with RT-PC...

Abstract:Objective To study effect of high fat diet on serum chemistry and homogenized tissue chemistry on mice,and to construct the animal model for human coronary artery disease or other metabolism syndrome.Methods\ Total 63 C57BL/6j mice were randomly divided to 2 groups,feed high fat diet and standard chow diet for 67 days respectively,the body weight,food intake,serum and homogenized tissue chemistry,morphological characteristic was studied.Results(Mice feeding) high diet showed improved total cholesterol and l...

Abstract:Objective To establish PCR testing method that monitor simian foamy virus in laboratory monkeys and related biological products.Methods Designed two series primers of nested PCR according to the region which has high homology among SFV-1,SFV-3,SFV_(CPZ) in pol gene of SFV proviral DNA,then cloned and measured sequence for SFV-1 strain after RT-nestedPCR to determine its accuracy.After tested its specificity and sensitivity,to test peripheral blood lymphocytes of rhesus monkey,passage monkey kidney cells and...

Abstract:Hepcidin,a small antimicrobial peptide synthesized predominantly in the liver,is the presumed negative regulator of iron homeostasis and plays a most significant role in the etiopathogenesis of the hemochromatosis and,as recently shown,anemia in chronic inflammatory diseases.Many evidences demonstrate that hepcidin directly controls the intestinal uptake of iron and leads to the iron retention within monocytes/macrophages.Meanwhile,hepcidin displays a broad spectrum of antibiotic activity against a variety ...

Abstract:Basic Calponin is a basic protein first isolated from chicken gizzard and bovine aorta.It is specifically expressed in smooth muscle and binds calmodulin,actin,and tropomyosin.It is able to inhibit the ATPase activity of myosin and is thought to play a role in smooth muscle contraction,signal transduction,cytoskeleton and cellular proliferation.

Abstract:How to control the quality of long-term toxicity test on rats is very important.It includes the quality control before and during and after the experiment.The key thing is dose-designing,while the core is practicing according to the good laboratory practice and the standard operating procedure,the most important and difficult is to control the quality of data-analysis and report.

Abstract:The SPF animal experiment room was built at the twelfth floor of department in institute of disease and control of PLA.It is about 240 m~2.The thesis introduced design in technology,as well as the building experiment.

Abstract:The reproductive rate of laboratory monkeys directly affects economic benefit of the laboratory monkeys producing company.This paper was based on years of laboratory monkeys breeding experience of the centre.a more comprehensive measures for improving the reproductive rate of laboratory monkeys was summed up,covering the reproductive characteristics of laboratory monkeys,males species selection,reproductive monkeys grouping and gradient feeding,pregnant monkeys medication,postpartum monkeys care,Juvenile mo...

Abstract:With the development of medical experimental animals science and the implementation of laboratory animal production and use sage license management approach,We summarized some experiences of barrier facilities operation and management in Laboratory Animal Center,Sun Yat-sen University,including complete management system establish,team building and management,laboratory barrier facilities maintenance,experimental items management in barrier facilities.

Abstract:Design of experimental animal facilities of energy-efficient building is the key factors which hinders the normal operation of facilities for the establishment and management.Good energy-saving measures including optimization of barrier systems and the layout of IVC cages,which will help to ensure that air quality of the experimental zone,save investment and improve the comprehensive utilization rate of facilities for 3 to 5 times.Using of Variable Air Volume Control Systems,can greatly reduce the thermal e...