Abstract:Chicken major histocompatibility complex(MHC) has been a research focus for its high polymorphism and close relation with varying resistance against diseases.It is located on a microchromosome(chromosome 16).It has been found that it has different resistance against diseases for different B haplotypes.The article gives a detailed introduction about structural characteristics,relation to diseases resistance,the progress in detection methods of chicken MHC,and the prospect of application of MHC in molecular b...

Abstract:目的观测G蛋白偶联受体激酶5（G protein-coupled receptor kinase,GRK5）在稳定表达hα-synuclein（人突触核蛋白）的SHSY5Y细胞的胞核、胞浆中的表达情况并对其在帕金森病中的可能作用进行研究。方法应用Western blotting、组蛋白去乙酰化酶（histone deacetylase,HDAC）活性检测技术以及shRNA干扰技术等对稳定表达hα-synuclein的SHSY5Y细胞中GRK5的表达及其亚细胞分布、胞核内GRK5蛋白的功能进行研究。结果发现GRK5蛋白在过表达hα-synuclein的细胞核以及细胞浆内均表达增加,胞核中的GRK5蛋白通过影响组蛋白去乙酰化酶的活性对bcl-2基因的转录和表达进行调控。结论帕金森模型中GRK5通过对bcl-2基因的表达进行调控发挥作用。

Abstract:Objective To establish a technologic platform for generating transgenic mice by lentivirus-mediated delivery of foreign gene(s) into zygotes.Methods\Monomeric red fluorescent protein(mRFP) transgenic mice were generated by subzonal injection of lentivirus harboring mRFP gene into single-cell fertilized eggs of ICR mice to infect fertilized eggs,and subsequently the embryos infected by lentivirus were transplanted into the pseudopregnant mice to attain F0 mice,followed by screening mRFP transgenic mice from ...

Abstract:Objective To investigate the possible effect of STK15 gene on the NIH3T3 fibroblasts.Methods\pcDNA3.1-STK15 expression plasmid was constructed and transient transfected into NIH3T3 cells.The effects of STK15 gene on the NIH3T3 fibroblasts was examined by RT-PCR,immunocytochemistry and Western blot,respectively.The cell proliferation rate was measured by MTT.The capability of cell invasion was observed by Transwell.Results STK15 expression was significantly upregulated in pcDNA3.1-STK15 plasmid-transfected N...

Abstract:Objective The anti-tumor peptide of human endostatin was cloned into eukaryotic expression vector pVAX1 and its bioactivity was assayed.Methods\The nucleotide sequence encoding 1-30 amino acids(30-peptide,sequence RGIRGAD was changed to RGDRDG) of human endostatin was synthesized and inserted into plasmid pVAX1.The signal peptide coding sequences of collagen XVIII was also linked to the 5'-end of target gene.Then the recombinant plasmid was directly injected into the tumor tissue in mice.ELLISA,immunohistoc...

Abstract:Objective To study the effect of active components from Bdellovibrio bacteriovorus metabolites on immune functions in mice.Methods\To inject the mice with ethyl acetate extraction(EAE),chloroform extraction(CE),petroleum ether extraction(PEE) and residual liquid(RL) of Bdellovibrio bacteriovorus metabolites,respectively.The changes of immune functions in the mice were investigated by detection of Hb value,RBC amount,SOD activity,agglutinating antibody titer,phagocytic activity and bactericidal activity ever...

Abstract:Objective to investigate the distribution of Fos-immunoreactive(IR) neurons in conscious rat brain after electrical stimulation of dura near superior sagittal sinus(SSS).Methods\The rats were prepared by exposure of the superior sagittal sinus.The dura mater near the SSS was electrically stimulated and Fos protein was labeled by immunohistochemical staining.Results After the electrical stimulation,the Fos-IR neurons were observed in many areas in the brain: the upper cervical spinal dorsal horn,spinal trige...

Abstract:Objective To establish a technique of random amplified polymorphic DNA(RAPD) analysis of genetic markers and to understand the population genetic diversity in tree shrews(Tupaia belangeri chinensis).Methods\Forty random oligonucleotide primers were optimized.Some of those could be used to effectively analyze the tree shrew RAPD loci were used to amplify the genomic DNA.The results were analyzed for the population genetic characteristics of the tree shrews with Popgene 1.32 and RAPDistance Package Version 1....

Abstract:Objective To study the effect of Benzo((a))pyrene() on lipid peroxidation and antioxidative activity in mouse liver and kindey.Methods\The malondialdehyde((MDA)) contents in the livers and kidneys were measured through thiobarbituric acid((TBA)) method.Ammonium molybdate chromatometry was used to measure the catalase((CAT)) in liver and kidney tisssures.Results B(a)P lead to a significant increase of MDA content in the mouse liver and kidney compared with that of the control group,especially in the group of...

Abstract:Objective To explore the optimal anesthetic method in surgery for Cynomolgus monkey.Methods\To summarize the characteristics of anesthetic methods with 4 anesthetic agents (Sumianxin,ketamine hydrochloride,isoflurane,and lidocaine) in surgery for cynomolgus monkeys.Results Good anesthesia was obtained in the monkeys with Sumianxin,ketamine hydrochloride or isoflurane,but not with lidocaine.Conclusions\To make sure biosafety and animal welfare,animal should be used in anesthesia,to choose appropriate anesthe...

Abstract:Objective To monitore the genetic quality of laboratory rats in Heilongjiang Province.Methods According to national standard of Laboratory Animal-Genetic Monitoring: methods for biochemical markers of inbred mice and rats GB/T 14927.1-2001,biochemical markers of 25 rats of SD,Wistar,DA,PVG strains from different sources were detected.Results There were differences among the strains.Conclusions\SD and Wistar rats in our province are in accordance with the genetic profile of closed colony animals.The genetic ...

Abstract:Objective This study intended to explore a new method to establish a model of heart ischemia-reperfusion in rabbits in vivo.Methods\Forty New Zealand white rabbits were randomly divided into two groups: ischemia-reperfusion group(n = 25) and sham operation group(n= 15). The myocardial ischemia-reperfusion were generated by two sutures and two knots,namely ligating the proximal 1/3 segment of the left anterior discending artery(LAD) for 30 minutes followed by opening the blood flow for 3 hours.The LAD of rab...

Abstract:Objective To establish a rapid SNP genotype assay for the leptin~(ob) and leptinr~(db) alleles((single-)tube bi-directional allele specific amplification((SB-ASA),) and compared it with the traditional method of PCR-restriction endonuclease digestion.Methods\Seven and nine offsprings of ob/+ and db/m heterozygous mice,respectively,were genotyped by both the SB-ASA and PCR-restriction endonuclease digestion.Results All the offsprings were successfully genotyped by both the two methods,and the results were id...

Abstract:Objective To explore the improvement of techniques of right atrial catheterization in rats.Methods\20 Sprague-Dawley rats were used for the right atrial catheterization surgery.The rats after surgery were randomly divided into 2 groups,10 in each group: Group A: the catheter was directly inserted inside the opening hole of the wire spring group,and group B: the catheter was inserted from the lateral side of the wire spring.The catheter was fixed in the body and pulled out from the neck back followed a fixed...

Abstract:Many identified functional genes in mammals are derived from mouse.There are about 140 mutations identified,since phenotype of cataract can be easily screened out.Spontaneous mutations are usually originated from large-scale animal breeding,and induced mutations are often obtained by radiation or ENU treatment.Sometimes,cataract can be found in knockout or transgenic mice.Known cataract genes,which are expressed in embryonic period,are known to be distributed on twenty chromosomes,and chromosome 1 harbors t...

Abstract:Tree shrew(Tupaia belangeri) is a newly developed animal model in medical research.The animal can be successfully infected with many human pathogenic viruses,and has been widely used in research in oncology,endocrinology,neurology and ophthalmology.Experimental animal models provide useful tools to better study the pathogenesis,prevention and treatment of many diseases.This article aims to review the recent advances of application of tree shrew in experimental medical research.

Abstract:Laboratory monkey measles is one of important infectious diseases which often causes death of weaned monkeys.In this study,clinical diagnosis,care,precaution and therapy protocol of the disease were explored and an effective prevention and care as well as therapy measures based on clinical experience was put forth.Clinical practice proved that these measures were very effective and recovery rate of measles was over 90%. This protocol is worthy to be popularized in laboratory monkey breeding and utilizing en...

Abstract:Objective To characterize the pathological changes of laboratory monkeys with bacterial diseases.Methods\Eighty four laboratory monkeys with bacterial infection were selected and divided into 5 groups according to the results of pathobiologic examination: group infected with Salmonella,group infected with Shigella,group infected with tuberculous mycobacteria,group infected with Yersinia enterocolitica and group infected with Campylobacter jejuni.Organs of the animals were processed for pathological examinat...

Abstract:Objective To investigate the natural infection rate of murine norovirus(MNV)in laboratory mice in Guangdong province.Methods\206 Mice were selected randomly from seven mice breeding facilities in this province for a survey of MNV infection rate.Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect MNV in the mouse cecal contents.Results 206 samples were tested and 77 samples were positive for MNV,the infection rate was 37.38%.The mice of three facilities were infected with MNV.There wer...