Abstract:The development of laboratory animal resource is not only a basic core work for the advance on laboratory animal science,but also one of the key support conditions for life science.Nowadays,with the rapid development of life science,the current status of laboratory animal resource used in practice is hard to meet the needs of scientific research in this field.Thus,it is imperative for us to develop new species and/or strains of laboratory animals for scientific purpose. The review introduces the existing st...

Abstract:目的研究贵州土家族、侗族、仡佬族和彝族人群线粒体DNA（mtDNA）编码区的核苷酸多态性。方法采用PCR-RFLP技术和DNA测序法对贵州4个群体145例样本mtDNA编码区的8个SNP基因座及COⅡ/tRNAlys基因间9 bp缺失进行多态性分析。结果贵州4个民族群体的9 bp缺失频率依次为土家族18.4%,侗族29.7%,仡佬族25%,彝族16.7%,平均缺失频率为22.8%;在8个SNP基因座中,A10398G、C10400T突变在4个群体中较普遍;A663G、C5178A和G12406A突变在部分民族群体中也有较高的频率;共检测出14种单倍型,其中仡佬族11种,土家族10种,侗族8种,彝族6种。结论贵州4个民族群体mtDNA编码区可能存在不同的突变热点,在等位基因和单倍型分布频率上存在一定差异。

Abstract:目的建立稳定表达红色荧光蛋白基因的人胰腺癌细胞系,为体内监测肿瘤的早期生长及抗肿瘤药物的药效评价建立一种新的肿瘤动物模型。方法以Lipofectamine 2000介导chickenβ-actin-RFP-NEO转染人胰腺癌细胞SW1990和Capan-2,经梯度浓度G418筛选获得稳定表达红色荧光蛋白的细胞克隆并扩大培养。BALB/cA-nu裸鼠皮下接种1×106个发光细胞使其成瘤,活体荧光成像系统观察肿瘤的生长情况。结果获得了稳定表达RFP的两种不同的人胰腺癌细胞株,将其接种到裸鼠体内可成瘤,利用活体成像系统观察了肿瘤的生长动态过程,并且SW1990肿瘤细胞的生长速度较Capan-2细胞快。结论用红色荧光蛋白标记的人胰腺癌细胞建立的裸鼠肿瘤模型为胰腺癌的研究和相关药物筛选提供了可进行荧光影像活体、动态分析的动物模型。

Abstract:目的观察βamyloid蛋白在不同年龄的恒河猴脑中的表达及其组织学和细胞超微结构水平的分布特点。方法分别取脑组织额叶、海马、颞叶和顶叶做免疫组化,观察βamyloid蛋白在组织学上的分布特点及与年龄的关系;选用23岁恒河猴一只,灌注后取上述部位做免疫电镜,观察βamyloid蛋白在细胞超微结构水平的分布特点。结果免疫组化染色可观察到年轻猴的神经细胞和胶质细胞中有少量的Aβ40颗粒,以额叶和海马居多。在老年猴脑中Aβ40常聚集成团状斑块。年轻猴脑细胞内未见明显的颗粒状Aβ42,而老年猴的额叶有多量的Aβ42细胞外散在斑块,神经细胞和胶质细胞内也见有Aβ42颗粒状沉积。免疫电镜可观察到Aβ40的胶体金颗粒大部分存在于老年猴神经细胞细胞质中,在细胞间质和小胶质细胞中也可见少量的胶体金颗粒,多见于额叶;标记Aβ42的胶体金颗粒也是多见于神经细胞中,在小胶质细胞中少量存在,同时在颞叶的神经纤维束中也可见Aβ42标记的胶体金颗粒。结论Aβ42是恒河猴老年斑的主要成分,并在其形成过程中起到重要作用。

Abstract:Objective To establish the pattern of needle electrodes by stimulating the soft palate in monkeys induced velopharyngeal muscle movement,and to achieve an effective stimulus values of soft palate movement.Methods Five sets of muscles of adult monkey's soft palate were anatomized to determine the surface location.The palate muscle electrode positioning stimulator and needle electrode were used to make an effective stimulating of adult monkey's soft palate muscles.The record muscle contraction and velopharyng...

Abstract:Objective To establish animal model and to support basic and clinical investigation of tourette syndrome(TS).Methods The SD rat was selected,and the model was established by iminodipropanenitrile(IDPN) peritoneal access for 7days.The animal model was evaluated by locomotion and stereotyped movement score,DA and NE of cerebrum determining with liquid chromatography method,and by morphology of neurons were observed.Results The model rats were presented the significant TS behavioral characteristic in locomotio...

Abstract:Objective To investigate the effects of ERK on asthma airway remodeling and the expressions of cyclinD1.Methods The rat asthma model was made through ovalbumin(OVA) sensitization and excitation.Primary cultures of ASMCs were established and cells between passages 3 and 6 were used for experiments.ASMCs were treated with ERK activator epidermal growth factor(EGF) and inhibitor PD98059,and then subdivided into 5 groups based on the drug interventions:(1)Normal control group;(3)Asthmatic control group;(3)Asthm...

Abstract:目的比较单侧输尿管结扎（UUO）与单侧输尿管结扎再通（RUUO）建立的SD大鼠肾纤维化模型的优劣,从而得到更符合临床慢性肾衰进展特点的动物模型,以供临床药物筛选、疗效评介、科研、教学使用。方法健康SPF级雄性SD大鼠54只,随机分成假手术组、UUO组及UUO再通组各18只,造模成功后检测血肌酐（Scr）、尿素氮（BUN）及尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）,病理检查肾小管间质纤维化指数和免疫组化检查α-肌动蛋白（α-SMA）。结果无论从肾功能方面,还是病理检查方面,UUO再通的大鼠肾纤维化动物模型均优于UUO大鼠模型。结论再通UUO肾纤维化大鼠模型在功能及病理方面及其相关的免疫组化方面优于UUO组,其病理特点更符合慢性肾小管间质纤维化进程的动物模型。

Abstract:Objective To develop a mice model of type 2 diabetes mellitus(DM) by high fat diet with low dose streptozotocin(STZ).Methods\ Male C57BL/6J mice at 5 weeks of age were randomly divide into conventional diet(n=8),conventional diet with STZ(n=8),high fat diet(n=8) and high fat diet with STZ group(n=8).After the exposure to conventional or high fat diet for 5 weeks,mice were injected intraperitoneally with 100 mg/kg of either STZ or vehicle solution during fasting state,and kept on the same diet for the next 4...

Abstract:Objective To observe the effect of inducible nitric oxide synthase inhibitor SMT on portal hypertension esophageal varices in rats.Methods\ 60 health male SD rats were randomly divided into five groups,sham operation group,model group,low dose SMT treatment group,middle dose SMT treatment group and high dose SMT treatment group.The portal vein and the left adrenal vein were not ligated in sham-operated group.The other groups were given a two-stage ligation of the portal vein plus ligation of the left adrena...

Abstract:Objective To develop selenium-deficient BALB/c mice by feeding with self-made selenium-deficient diet.Methods\ A selenium-deficient mouse food was prepared with local yeast.Young BALB/c mice were fed with the selenium-deficent diet for over 4 months.Selenium concentrations in the blood,liver,and myocardial tissue of the mice fed with selenium-deficient diet were examined.The myocardial enzymes in the treated mice were examined.The ultrastructures of the treated mice myocardial tissues were also observed.Res...

Abstract:Objective To study the dynamic situation of germ about laboratory animals'barrier system.Methods Using cotton pledget to test the concentration of bacteria in different area of barrier system,as well as through the method of airfall bacteria.Results The results showed that in dynamic enviroment,especially in animal laboratory rooms,the airfall bacteria was far more than static condition which has been limited below 3cfu/dish in the National Standards for Laboratory Animal Environment and Facility(GB14925-20...

Abstract:目的探讨黄芪多糖对暴露于苯、甲醛环境中小鼠脾脏的保护作用。方法将40只雄性小鼠随机分成4组,即对照组、苯甲醛染毒组（苯：5 g/m^3,甲醛：50 mg/m^3）、苯甲醛染毒黄芪多糖低剂量保护组[黄芪多糖：10 mg/（kg.d）,苯：5 g/m^3,甲醛：50 mg/m^3]、苯甲醛染毒黄芪多糖高剂量保护组[黄芪多糖：20 mg/（kg.d）,苯：5 g/m^3,甲醛：50 mg/m^3]。采用静式吸入染毒,每天2 h,连续30 d,并同时采用饮水的方式给予黄芪多糖保护。末次染毒24 h内,处死小鼠,对小鼠脾中谷胱甘肽（GSH）含量及谷胱甘肽过氧化物酶（GSH-PX）活性进行测定和分析。结果苯、甲醛染毒黄芪多糖保护各组脾组织中GSH含量和GSH-PX活性均高于苯、甲醛染毒组,均有统计学意义（P〈0.01）而与对照组的差异无统计学意义（P〉0.05）,苯、甲醛染毒黄芪多糖保护组高剂量组脾组织中GSH-PX活性高于低剂量组,差异有统计学意义（P〈0.01）。结论黄芪多糖对暴露于苯、甲醛环境中小鼠脾脏具有保护作用,且对脾中GSH含量的作用有随剂量增加而增大的趋势,对脾中GSH-PX活性的作用随剂量增加而增大。

Abstract:Objective To explore the effect of protein powder on immunological function.Methods\ The BALB/c mice were divided into three groups,and each group was divided into four groups.And individually to test the ratio of spleen-thymus/body weight,T lymphocyte proliferation of mouse spleen induced by ConA,the response of delayed hypersenitivity,the amount of hemolytic antibility in serum, antiboty-producing cell assay,phagocytic activity of macrophages and the ability of macrophages for carbon particle clearance an...

Abstract:Objective To compare the serological detection sensitivity of sera against HSV-1 and BV from humans and rhesus monkeys with PR(plaque reduction)and IFA(immunofluorescence assay).Methods The PR assay described in this study was modified from the traditional viral plaque reduction assay by using with 1 mL of DMEM medium containing 1%(weight/volume) of methylcellulose(4000 cP;Sigma,MC),and an improved crystal staining method to achieve better plague formation in HSV-1 infected Vero E6 cells.The neutralizing an...

Abstract:Objective To compare three detections for B virus antibodies,and to find an accurate,reliable and economical method.Methods B virus antibodies were detected with enzyme immunoassay(EIA,HSV-1 antigen),enzyme immunoassay(EIA,B virus antigen) and enzyme-linked immunosorbent assay(ELISA,B virus antigen) in macaque sera,and the testing results were compared.Results Consistency of testing results of EIA(HSV-1 antigen)with EIA(B virus antigen)and ELISA(B virus antigen) were 97.7% and 95.5%.Conclusion There is much...

Abstract:Skin irritant test was the routinely conducted for human healthy related product hazard assessment.Traditional skin irritancy testing used lab animals,costly,time-consuming and suffering from stress.Recent years,diverse alternative models for skin irritancy have been developed and applied.The effects in vivo were predicted in vitro testing by quantitative measured cell viability and biomarkers for metabolism disturbing.The biomarkers for skin irritancy included viability,inflammation cytokines,enzymes,morph...

Abstract:Clinical symptom is main basis of disease diagnosis.Non-human primates can not use language to express itself sense which is discovered by observing external clinical symptom.In order to diagnose disease in veterinary clinical,veterinarians must know the relations between various clinical symptom and disease.According to working practices,the paper summarized relations of general clinical symptom and primary disease diagnosis in non-human primate laboratory animal,it includes the following aspects:circulati...